FastQCFastQC Report
Sat 14 Mar 2020
HCCCVBGXF_n01_DG4595_ADI4_miRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCCVBGXF_n01_DG4595_ADI4_miRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9895619
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC388750.39285061399393006No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA369810.37371083102532543No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA327850.331308228419061No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG300180.303346359636522No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT158280.15994956960246753No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC152680.15429049966454852No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT137770.1392232259548392No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG110830.11199905736063605No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA1028450.069.5810551
GGATAGG510800.069.5058514
CGTAAAT478700.069.435869
TATTGGC481200.069.4245815
GTAGGTT690200.069.391728
AGTAGGT690950.069.382257
GTTGTAT502000.069.3783312
GATAGGC240250.069.37276515
GCAGCAC542050.069.343083
AGGTAGT1133400.069.325973
GGTAGTA1135800.069.324354
TGGTACG254450.069.3110913
GAGGTAG1153050.069.310392
TTGGCGA375000.069.2898617
GGTACGC249400.069.2694414
CAGCACG477100.069.266324
AAGTAAT530900.069.2498554
TAGTAGG692300.069.2259146
GATAGGT147650.069.21693415
CAGGATA528300.069.1839712
Produced by FastQC (version 0.11.5)