FastQCFastQC Report
Sat 14 Mar 2020
HCCCVBGXF_n01_DG4593_AP4_miRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCCVBGXF_n01_DG4593_AP4_miRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10318362
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA359670.348572767654401No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA348620.33786370356070083No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG282400.2736868506842462No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC236840.22953255565175945No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT179170.17364190168943483No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT142480.13808393231406302No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG133350.12923562867827276No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC121500.11775124772711017No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGG545350.069.6519214
TGAGGTA1138700.069.6492541
GTAGGTT745900.069.621778
AGTAGGT746650.069.5615547
GATAGGC255900.069.5608115
GATAGGT160100.069.4519515
GGTAGTA1245000.069.44354
AGGTAGT1242350.069.441943
TATTGGC512800.069.4388115
CGTAAAT510700.069.438539
GTTGTAT547600.069.4306512
TAGTAGG748600.069.392166
GCAGCAC574650.069.383223
GTATGGT259150.069.3772315
GAGGTAG1265050.069.3439942
CAGGATA566050.069.31315612
CAGCACG508100.069.307464
GTAAATA513350.069.2979210
GCACGTA510500.069.291896
TTCAAGT553900.069.288281
Produced by FastQC (version 0.11.5)