FastQCFastQC Report
Sat 14 Mar 2020
HCCCVBGXF_n01_DG4579_AM3_miRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCCVBGXF_n01_DG4579_AM3_miRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8542205
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA282680.33092158289340984No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG259690.3040081571444375No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC255870.29953624386209415No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA250680.2934605292193292No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT158840.18594730517471778No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT133390.1561540609245505No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG89570.10485583054960633No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC85580.10018490541961941No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGG544750.069.67090614
TGAGGTA997100.069.644441
AGGTAGT1161200.069.450633
GATAGGC272200.069.4456915
GGTAGTA1165300.069.432014
GAGGTAG1184250.069.4198762
AGTAGGT717000.069.394917
GTAGGTT716600.069.394178
GATAGGT146350.069.35287515
TTCAAGT545000.069.335711
GTTGTAT518450.069.32390612
CCAGGAT412200.069.3112211
AGGATAG563100.069.283813
CAGGATA564650.069.2737712
TAGTAGG719000.069.273276
AGATTGT282550.069.2685610
AAGTAAT567450.069.240484
GTAGTAG1166500.069.2255255
GGTACGC285450.069.1893514
GCAGCAC355300.069.170053
Produced by FastQC (version 0.11.5)