FastQCFastQC Report
Sat 14 Mar 2020
HCCCVBGXF_n01_DG4567_ADI3_miRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCCVBGXF_n01_DG4567_ADI3_miRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11693467
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA460400.3937241196302174No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA363990.3112763733801105No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG348840.29832042113771734No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC307590.26304431354704294No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT192290.1644422479663217No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC156900.1341774855994377No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT149560.12790047639421226No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG118850.10163794877943386No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGG644100.069.6341614
TGAGGTA1249800.069.620811
GATAGGC313150.069.5511615
GATAGGT173600.069.4538915
AGTAGGT841100.069.4141857
AGGTAGT1376950.069.400283
CGTAAAT465900.069.3915259
GTAGGTT841700.069.3810358
TATTGGC470550.069.3808815
GGTAGTA1380100.069.35414
GAGGTAG1403300.069.325632
TAGTAGG842700.069.306696
GTATGGT291800.069.29056515
GTTGTAT614250.069.285712
AAGTAAT669200.069.278374
GCAGCAC534900.069.274533
GGTACGC289050.069.2717314
CAGCACG463600.069.258544
CCAGGAT487550.069.23711
TTCAAGT653350.069.20411
Produced by FastQC (version 0.11.5)