FastQCFastQC Report
Sat 14 Mar 2020
HCCCVBGXF_n01_DG4565_AP3_miRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCCVBGXF_n01_DG4565_AP3_miRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10351519
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC456120.44063098372325843No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA392690.379354952640284No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA320650.3097613016988135No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG302970.29268168275593176No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT184410.17814776749190145No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT153360.14815216974436313No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC132590.12808748165365874No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA1038450.069.6375661
GGATAGG525000.069.5515814
TATTGGC558900.069.4784815
GCAGCAC630150.069.4584053
GATAGGC246600.069.4305315
GTTGTAT494050.069.4247512
CGTAAAT562050.069.408349
AGTAGGT676200.069.405367
CAGCACG558950.069.387934
TGGTACG267000.069.3821413
GTAGGTT675800.069.379118
GTATGGT235700.069.3745715
GGTAGTA1144050.069.328954
GAGGTAG1159950.069.328032
AGGTAGT1142400.069.2932363
TAGCAGC607900.069.2928851
TAGTAGG677800.069.287766
TAGGTTG676600.069.275719
GGTACGC262850.069.2658914
GATAGGT157700.069.2436515
Produced by FastQC (version 0.11.5)