FastQCFastQC Report
Sat 14 Mar 2020
HCCCVBGXF_n01_DG4478_AP2_miRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCCVBGXF_n01_DG4478_AP2_miRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10386790
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG277230.26690633005962383No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC257930.24832503593506752No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA187010.18004600073747518No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA174610.16810775995278618No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT142840.1375208317487886No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG125940.1212501648728818No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGG619350.069.6255614
TGAGGTA1113650.069.610741
GATAGGT172850.069.57297515
GTAGGTT747650.069.452498
GTTGTAT542200.069.43014512
GCAGCAC550500.069.428653
GATAGGC297850.069.4224115
AGTAGGT748850.069.420327
CGTAAAT481650.069.403679
TATTGGC487200.069.3803915
AGGTAGT1239750.069.367773
GGTAGTA1242200.069.3506554
GTATGGT258700.069.3217515
GAGGTAG1260350.069.320082
AAGTAAT641850.069.3068854
CCAGGAT462950.069.30266611
TAGTAGG751000.069.289536
TTCAAGT625800.069.286551
CAGGATA642300.069.27891512
TAGGTTG749700.069.266589
Produced by FastQC (version 0.11.5)