FastQCFastQC Report
Sat 14 Mar 2020
HCCCVBGXF_n01_DG4476_AM2_miRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCCVBGXF_n01_DG4476_AM2_miRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12126806
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG362850.2992131646205934No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA285980.2358246680947976No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT225420.18588571467210738No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA187460.1545831606442785No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT172560.14229633095474603No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG126440.10426488227815303No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC124210.10242598092193443No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA1458300.069.670391
GGATAGG781200.069.6267114
GATAGGT207500.069.5600215
GTAGGTT1005900.069.523038
GGTAGTA1612850.069.483484
GTTGTAT732750.069.4725912
AGGTAGT1609250.069.471123
AGTAGGT1007200.069.464587
GATAGGC387450.069.3843115
GAGGTAG1643650.069.363032
TAGTAGG1009350.069.357716
CAGGATA807850.069.3481312
GTATGGT359100.069.3455615
TATTGGC471650.069.3158715
AAGTAAT811100.069.303134
GCAGCAC541250.069.301373
TAGGTTG1008450.069.298079
TTCAAGT791350.069.270831
GTAGTAG1616000.069.238125
CCAGGAT593950.069.2266311
Produced by FastQC (version 0.11.5)