FastQCFastQC Report
Sat 14 Mar 2020
HCCCVBGXF_n01_DG4474_ADI2_miRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCCVBGXF_n01_DG4474_ADI2_miRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9097761
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG288110.31668231337358715No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC173690.19091510537592712No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA169320.18611172573119913No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA157270.17286670863303621No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT144000.1582807022519057No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT116540.12809745166970202No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG105230.11566582151366694No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA1078200.069.677571
GGATAGG568150.069.579814
AGGTAGT1218100.069.522073
GATAGGC267350.069.5139415
GTAGGTT749500.069.5009468
GGTAGTA1221950.069.495364
AGTAGGT749850.069.4871757
GAGGTAG1241150.069.420492
GTTGTAT546100.069.40266412
CCAGGAT429900.069.372211
CAGGATA587600.069.343512
TAGTAGG752300.069.342416
GTATGGT266450.069.3284115
CAGCACG342450.069.3216254
GATAGGT153200.069.2901115
GTAGTAG1221300.069.283345
AAGTAAT590000.069.281014
TTCAAGT572100.069.256471
CGTAAAT345350.069.2502759
TAGGTTG752000.069.241599
Produced by FastQC (version 0.11.5)