FastQCFastQC Report
Sat 14 Mar 2020
HCCCVBGXF_n01_DG4460_ADI1_miRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCCVBGXF_n01_DG4460_ADI1_miRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10790181
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC356530.33042077792763624No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG353270.3273995125753683No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA342380.31730700346917257No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA320230.29677908090698385No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC184880.17134096267708576No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT183030.16962644092809934No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG155580.1441866452471928No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG116630.10808901166718149No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA1140850.069.629361
GGATAGG585600.069.6214714
CGTAAAT448300.069.523379
GATAGGC274950.069.476115
CAGGATA607550.069.3876812
GTAGGTT762700.069.375848
GAGGTAG1282200.069.37232
GGTAGTA1261100.069.371084
AGTAGGT763450.069.358447
AGGTAGT1258550.069.3579943
GCAGCAC514950.069.340193
TATTGGC454250.069.3122715
TAGTAGG765650.069.298236
GTTGTAT558600.069.2903112
GAAGTAT322750.069.26092512
AAGTAAT611100.069.2587364
TGGAATG337900.069.210931
CAGCACG447700.069.19964
GATAGGT164650.069.1902515
GTAGTAG1261250.069.179635
Produced by FastQC (version 0.11.5)