FastQCFastQC Report
Sat 14 Mar 2020
HCCCVBGXF_n01_DG4450_AM1_miRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCCVBGXF_n01_DG4450_AM1_miRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10791103
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG343770.31856799068640157No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA178940.1658217885604465No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT160300.14854829946484618No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC150380.13935554131954814No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA134390.12453777894623007No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT133760.12395396466885729No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG121430.11252788524027618No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG119440.11068377347524158No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC110090.10201922824756653No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA1251000.069.592231
GGATAGG702250.069.59074414
GATAGGT175500.069.50085415
GTAGGTT849000.069.499968
AGGTAGT1384250.069.494723
GGTAGTA1387900.069.480964
AGTAGGT849700.069.4515847
GATAGGC365950.069.444715
CGTAAAT430200.069.404569
GGTACGC368100.069.3623714
GTAGTAG1388100.069.349895
TAGTAGG851800.069.34426
GTTGTAT619000.069.34289612
TTCAAGT707400.069.3395841
CAGGATA726750.069.3341812
GAGGTAG1413650.069.3319242
TAGGTTG851750.069.3163459
CCAGGAT544350.069.3043811
GTATGGT294250.069.2738615
TGGTACG375350.069.26214613
Produced by FastQC (version 0.11.5)