FastQCFastQC Report
Sat 14 Mar 2020
HCCCVBGXF_n01_DG4446_AP1_miRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCCVBGXF_n01_DG4446_AP1_miRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11188496
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA375670.3357645209865562No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG331930.29667079471628716No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC315310.2818162512637981No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA279270.24960459386140907No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT164150.14671319541071473No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG143690.12842655527606212No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC139950.12508383611166327No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT127940.11434959622812575No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG112180.10026369942841289No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGG650650.069.6649314
TGAGGTA1262850.069.634311
GATAGGT183050.069.5395515
GGTACGC332800.069.4515814
AGGTAGT1383000.069.437893
GGTAGTA1386650.069.429934
TGGTACG338800.069.3995813
AGTAGGT853800.069.398337
GATAGGC310300.069.3893615
TAGTAGG853850.069.3804556
CAGGATA674250.069.3605912
GTAGGTT854050.069.353128
TTCAAGT661200.069.336391
CGTAAAT439600.069.331219
TTGGTAC339350.069.32867412
GTTGTAT619650.069.3269212
GAGGTAG1413600.069.301482
AGGATAG674000.069.2717613
TATTGGC447100.069.25477615
CCAGGAT485350.069.2058311
Produced by FastQC (version 0.11.5)