FastQCFastQC Report
Thu 5 Mar 2020
HC7WWBGXF_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC7WWBGXF_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27388923
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG303254211.072147670793774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAT17800.012.4331091
CCCGTAT8800.011.5764281
TCGGTTT24500.010.4383492
CCCGGAT23350.010.3805751
GTCCGTA6452.4410838E-99.8032941
CTCCCGT16950.09.7406381
CTCGGTT27700.09.5113181
TATCGCG3500.0018274768.9989169
GTCCGGA13350.08.9465761
TACGTCG7453.246896E-98.92643526
TTACCGG9102.0008883E-118.844838
ATACGTC14700.08.80891219
CCCGTTT21800.08.7015481
CCGCTAT5251.2976356E-58.6984791
CCCAGAT44900.08.6060841
CGGTTTT24500.08.572793
CCCGTGT20100.08.5636571
CCCCGAT15300.08.495121
TCCGCTA4956.367832E-58.4928042
CGCCTAT4552.317189E-48.4925981
Produced by FastQC (version 0.11.5)