FastQCFastQC Report
Thu 5 Mar 2020
HC7WWBGXF_n01_BMDM_S3_M3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC7WWBGXF_n01_BMDM_S3_M3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52687610
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG8450.011.59746559
CTCGTAT74750.011.3312071
CAACCGG52600.011.04697631
CCCGTAT60650.010.61841
AGCCCGC151600.010.18150114
CGATTCG25850.010.15440844
CCCGTTT123000.09.9025311
TCGACCG23000.09.89251331
CGCGTAA17400.09.65478570
GTCCATT227700.09.4533141
GCAACCG61750.09.41000330
CTCCGAT99100.09.1827281
CTCCGGT159250.09.1429411
GTCCGGT62900.09.1256811
GTCGTTT94900.09.1096731
GTCCGTT76900.09.1028141
GCGTACG37850.08.9698723
CCCGGTT137350.08.9698681
CTCCATT506550.08.94786551
TCGTATC60650.08.8868442
Produced by FastQC (version 0.11.5)