FastQCFastQC Report
Thu 5 Mar 2020
HC7WWBGXF_n01_BMDM_S3_M2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC7WWBGXF_n01_BMDM_S3_M2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35652618
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT53500.012.8225031
CGCGTTA33400.012.0518834
CTAGCGG34100.011.90627738
CGACGCG23700.011.8151128
CTCCGGT107350.011.63956451
ACTATCG29450.011.52772358
AGTTACG58250.011.4759456
TCGACCG31200.011.21944531
TACGATA40600.011.12045659
CCCGTTT92050.011.1027251
ACGATAC41350.011.08802360
GTTACGG61150.011.0460367
TACGACT29200.011.0269666
GTTACGA31800.010.89584
CCCGTAT34750.010.877761
CTCGCAT64750.010.8649261
AGCCCGC98350.010.81855314
CTCCGAT64100.010.8112951
CCGTACG26600.010.7890514
TTCGCGA11750.010.72351138
Produced by FastQC (version 0.11.5)