FastQCFastQC Report
Thu 5 Mar 2020
HC7WWBGXF_n01_BMDM_S3_M1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC7WWBGXF_n01_BMDM_S3_M1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38798364
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG24950.019.35817348
CGTATGC39750.012.5028146
TCGTATG39250.012.03789445
TTAAGCG22850.011.79397949
ATCGTTA26850.011.07977745
CCCGTAT35100.010.5698841
CTCGTTT99750.010.5263991
CTCCGGT118200.010.2454281
CCCGAAT54800.010.219061
CCCGTTT94550.010.10584451
CGCGTAA9700.010.10274370
AGCCCGC116550.010.00011814
CTCCGAT75350.09.8939081
CCCGGAT113100.09.7790351
CGTTAAG28900.09.6883147
ACGATAC43450.09.50494860
TACGATA43750.09.35977259
CTCCCGT78750.09.2445181
CCCCGAT66050.09.167371
CCCGGTT90200.09.0798941
Produced by FastQC (version 0.11.5)