FastQCFastQC Report
Thu 5 Mar 2020
HC7WWBGXF_n01_BMDM_S1_M3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC7WWBGXF_n01_BMDM_S1_M3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33998723
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAC38150.011.00890260
TCGGTTT107150.011.0076282
CCCGTTT84400.010.9478051
CTCGTAT47800.010.836761
CTCGTTT88450.010.5652321
CGTATAG16700.010.47901319
GACGTAT17050.010.46911817
CTCCGAT88850.010.36011
CGATTCG22300.010.35850344
GTCCGGT41700.010.3236831
GTCGGTT69200.010.3178621
CCCGTAT37450.010.1868591
GTCCGTT44050.010.1702031
ACGTATA16950.010.11799618
GTCCGAA34800.010.0574151
CTCGGTT113450.09.9338361
CGCTAGT12150.09.7941841
CCGCTAA31700.09.71669229
CAACCGG30750.09.67596631
TCGTTTT104050.09.58647252
Produced by FastQC (version 0.11.5)