FastQCFastQC Report
Thu 5 Mar 2020
HC7WWBGXF_n01_BMDM_S1_M2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC7WWBGXF_n01_BMDM_S1_M2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56148102
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAT26900.014.57252517
ACGATAC68500.012.8755360
CGTATAG30050.012.69552619
ACGTATA31300.012.6358818
CTCGTAT80500.012.0435711
AGCCCGC134750.011.35079714
CTCGTTT141600.011.2959911
CCCGTAT62200.011.1978351
TCGGTTT189050.011.1818742
GACTACG35850.011.13066535
CCCGGTT146600.011.0539731
CGATACT80350.010.9766461
CTCGGTT200000.010.8850831
CATACGA80500.010.86921457
CTCCGAT145150.010.8509281
CAACCGG51700.010.8333231
GCATACG81150.010.73887156
CGATTCG35200.010.7382644
CCCGTTT140650.010.7004021
CTCCGGT148200.010.5567611
Produced by FastQC (version 0.11.5)