FastQCFastQC Report
Thu 5 Mar 2020
HC7WWBGXF_n01_BMDM_S1_M1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC7WWBGXF_n01_BMDM_S1_M1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29238732
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAC34700.014.92767560
TATCGCG5450.014.12875622
GACGTAT12800.013.12515817
GCATACG41250.012.47238756
CATACGA41200.012.40272357
CGATACT42600.012.07723861
CCCGTAT28350.011.60495951
CTCGTTT78000.011.217971
AGCCCGC75100.010.95219714
TTACCGG20800.010.9371088
TACGATA46800.010.91861859
CGTATAG14550.010.82487319
TCGGTTT95800.010.7042822
CTCGTAT40200.010.621911
ACGTATA16150.010.61930518
GACTACG17300.010.5208835
CCCGTTT72800.010.4327121
TACGTCG21750.010.29929326
CTCCATT271750.010.2649681
GTCGGTT48950.010.2247371
Produced by FastQC (version 0.11.5)