FastQCFastQC Report
Thu 5 Mar 2020
HC7WWBGXF_n01_BMDM_N_M3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC7WWBGXF_n01_BMDM_N_M3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41496484
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGG47050.013.98719131
CGCTACG17200.012.61600159
CGCGTAA19850.012.34201570
ACGATAC46750.012.0532160
GCAACCG53750.011.98305130
TCGACCG24950.011.92561231
CCCGTTT105700.011.7550621
GACGTAT19200.011.66674717
TCGGTTT143400.011.39784052
CGACCGT27150.011.34606132
AACCGGA61100.011.114573532
CTCCGAT93250.010.9598741
TTCGCGC13500.010.8883097
CGTATAG20050.010.64845319
GTTCGAT75850.010.6131381
TATCGCG6957.2759576E-1210.57563922
CTCGCAT56950.010.5707621
ACGCCGT33550.010.53642111
GTCCATT181850.010.4702531
CCGCTAA46750.010.40712829
Produced by FastQC (version 0.11.5)