FastQCFastQC Report
Thu 5 Mar 2020
HC7WWBGXF_n01_BMDM_N_M2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC7WWBGXF_n01_BMDM_N_M2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32790029
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGG34400.015.36537631
ATTACGC13100.013.6252377
ACGATAC39200.013.21403360
TCGGTTT118300.012.6623072
CTCCGAT75350.012.2627451
GCAACCG44250.012.18228630
CTCGTAT42850.012.0886541
CTCGGTT129750.011.73407651
CCCGATT25800.011.6666391
CCCGTAT49900.011.643261
CGATTCG19700.011.54793344
AACCGGA46700.011.39334832
CTCCGTT72850.011.2902971
CGATACT45800.011.15700961
GTCCGTT41100.011.1556921
GTCCATT141900.011.1486711
CGTATAG16100.011.08686319
TACGATA46300.011.03650759
CATACGA48250.010.95307457
CCCGTTT87800.010.7630731
Produced by FastQC (version 0.11.5)