FastQCFastQC Report
Thu 5 Mar 2020
HC7WWBGXF_n01_BMDM_N_M1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC7WWBGXF_n01_BMDM_N_M1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33909057
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTTT123600.012.5441422
ACGATAC39800.012.39921960
CTCGGTT135500.012.14021
GACGTAT15900.011.886806517
CTCGTAT47750.011.8743251
CTCCGAT79600.011.7838981
CAACCGG37550.011.6527631
CCCGATT25150.011.5506751
CGGTTTT114700.011.1983293
CCCGTTT88150.011.11739351
GTCCGGT37550.010.8122311
TATCGCG5209.511496E-910.7690849
CGTATAG17100.010.64317719
GCATACG47800.010.54355756
CCCGTAT35950.010.5145851
CATACGA46700.010.49223857
TTACGTA26400.010.34049469
GTCCGAA36100.010.276991
ACGTATA17950.010.13928818
CGATACT48750.010.05105761
Produced by FastQC (version 0.11.5)