FastQCFastQC Report
Thu 5 Mar 2020
HC7WWBGXF_n01_BMDM_H_M3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC7WWBGXF_n01_BMDM_H_M3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36014812
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT50550.012.9476651
CAACCGG35400.012.75581431
CCCGTTT93050.012.1118511
CCCGTAT47950.011.6789131
GCAACCG42600.011.01065730
TATCGCG5107.01948E-910.9806822
CTCGTTT85450.010.8953351
GTCCATT159300.010.700011
GTCCGTT49250.010.6599711
TCGGTTT98350.010.6403032
CCCGGTT89400.010.4922421
CTCCGTT76400.010.2160391
CTCCGAT61800.010.137611
CCCCGCT100600.09.9503671
CTCCATT325300.09.9093831
CCCGGAT97350.09.7432611
CTCGGTT118600.09.7386841
GTCCGGT38150.09.6330941
CCCGAAT48850.09.6008841
TACGATA31300.09.50456759
Produced by FastQC (version 0.11.5)