FastQCFastQC Report
Thu 5 Mar 2020
HC7WWBGXF_n01_BMDM_H_M2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC7WWBGXF_n01_BMDM_H_M2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32867636
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGA33850.018.92205638
TCGACCG59800.018.08764831
CGACCGT61000.017.9039832
GTTCGAC66650.016.49014929
GCGACGT64650.015.80836322
TTCGACC70700.015.49707630
TTCGCGC39300.015.2281957
ACGTCGC68750.014.91681625
AAGTTCG76150.014.70877427
ACGTTCG46000.014.6842666
CTAGCGG72050.014.57347838
GTTACGA39700.014.4579524
ACCCCGG69150.014.37397870
GATCGTG102750.014.34012952
ATAACGC91650.014.3217228
GACGTCG72400.014.30969524
CGCATCG93400.014.16513938
CGACGTC72350.014.12605523
AACGCCG92100.014.06264130
ACGACTT71050.014.0395351
Produced by FastQC (version 0.11.5)