FastQCFastQC Report
Thu 5 Mar 2020
HC7WWBGXF_n01_BMDM_H_M1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC7WWBGXF_n01_BMDM_H_M1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46654492
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA15400.011.13585870
TATCGCG7750.010.83893822
CCCGTAT47050.010.4144491
CTCGTAT61100.010.0245461
CCCGTTT120600.09.373961
GTCCGTT61700.09.3598031
CTCGTTT115350.09.2847811
GTCCGGT59700.09.1457261
ACGCCGT36650.08.976727511
CTCCGAT83650.08.912131
CAACCGG46700.08.84486531
TCGACCG22350.08.77074631
CCCGGAT125050.08.7604921
GTCGTTT73550.08.6607711
CCCGGTT108400.08.6208451
TATGCCG25250.08.59382648
GCCGTAT77850.08.4971071
CTCCGGT141450.08.4623521
CCCGAAT66200.08.4592111
TCGTTTT120850.08.4564982
Produced by FastQC (version 0.11.5)