FastQCFastQC Report
Sat 11 Jun 2022
HC5NHDRX2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC5NHDRX2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences830419037
Sequences flagged as poor quality0
Sequence length24
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCTCGGTGGTCGCCGTATCAT143003141.7220599917436623Illumina Single End PCR Primer 1 (100% over 21bp)
CGATCTCGGTGGTCGCCGTATCAT57509270.6925331361352208Illumina Single End PCR Primer 1 (95% over 24bp)
CGGTCTCGGTGGTCGCCGTATCAT33441230.402703075314975Illumina Single End PCR Primer 1 (100% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGG21103460.25413025303753967No Hit
CAGACGCGTGGCGTAACAGGTTGT11068870.13329258490975562No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)