FastQCFastQC Report
Sat 11 Jun 2022
HC5NHDRX2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC5NHDRX2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences830419037
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTAAGA13256312915.963401980631616No Hit
CCTACCAT12693327515.285448592142522No Hit
AAACGGCG11906046314.337395663534144No Hit
GGCGTTTC11091329713.356304715832279No Hit
CAATCCCT752725879.064410092515738No Hit
TGTGTATA630468557.592173612465005No Hit
ACGAGTAG606063097.298280301827907No Hit
GTCCAGGC482307555.8080020870234454No Hit
GGGGGGGG346287134.1700287995685725No Hit
TTCTAATA10711130.12898463935383023No Hit
AATAAAAA10564060.12721360577382812No Hit
GTGTAAGA10141110.12212039401982064No Hit
GGCGGTTC9953980.1198669533872933No Hit
CCAAAAAA9622170.11587125982517668No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)