FastQCFastQC Report
Sun 21 Jul 2024
HC5KGDRX5_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC5KGDRX5_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1022468531
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCTCG16829293916.459473704819576Illumina Single End PCR Primer 1 (100% over 8bp)
AATCTCTC286357452.800648052414241No Hit
TTCACGCA284538752.782860707915518No Hit
GGTCTATG272940752.6694293440313226No Hit
ATGCCTCA264305022.5849697275426466No Hit
GTTAACCG241859912.3654508932754625No Hit
CCTGAACT222894762.179966945114715No Hit
CGTAAGGA222248232.1736437187229187No Hit
GGCTAACA207074542.02524120519852No Hit
CATGGCCA195781291.9147903731425453No Hit
GGAGTTCC194642551.9036532088634033RNA PCR Primer, Index 1 (100% over 8bp)
GGACGTAT179379211.7543738957380195No Hit
CACCTACA179126601.7519033062544203No Hit
CAGTTCCT169647351.6591938515122868No Hit
CTACACTA166637211.629753923448623No Hit
AGCTCTAG165622751.619832248900773No Hit
TTGGTGCT161862851.5830594790207777No Hit
CGTACGCA157215691.537609082660364No Hit
GTCTGAGG153192511.498261368006836No Hit
GCATTGTC149764391.464733490169391No Hit
TAGCGACG147497651.4425642015186873No Hit
CACGTCGT146997011.4376678161061174No Hit
GTGTATAT140581271.3749202614823555No Hit
GAGGCTAT140500491.3741302127175197No Hit
CACGACAT134432061.3147794374514594No Hit
CCACGCGT124527081.2179062359817507No Hit
ACTAGAGC119671111.1704136251798247No Hit
TAACCGCG114209681.1169994629399504No Hit
TCAGTGTC110688171.0825582073586577No Hit
AACCGTGA96829650.9470183879918354No Hit
AGAGGATA94244260.9217326220086866No Hit
GTTAGTGC92937070.9089479742628871No Hit
TGAACCGA91812480.8979492005509947No Hit
TTCTTCCG86739260.8483318299798118No Hit
GCGATCTA85017370.8314913116871272No Hit
TTGTGCGT84648510.8278837678966181No Hit
CCTATCAC84226600.823757381732074No Hit
GTTCCTAG83389590.8155712129198038No Hit
CTGACGTG79609050.7785965786364137No Hit
TCTCGAAC79468550.7772224532160198No Hit
GTCGGTAA78561490.768351177743973No Hit
ATAGAGAG77227950.7553088203552741ABI Solid3 Adapter B (100% over 8bp)
CCAATGCT76766320.7507939625772209No Hit
CCATCTTA76277090.7460091698411401No Hit
GAGTAGCC75969850.7430042851851839No Hit
GTTGTGAC75445510.7378761077977861No Hit
TCATTACA75334350.7367889349740779No Hit
TCTACTCT73887610.7226394530473819No Hit
CTCCTTAC73205940.7159725485967059No Hit
TATGCAGT73186570.7157831051134815No Hit
CGAGAGTT72586440.7099136824192392TruSeq Adapter, Index 13 (100% over 8bp)
TCAGGCAA72522310.7092864748519091No Hit
GAGCTAAC71505140.6993382958208618No Hit
TGTTACGG70760350.6920540618574793No Hit
TTCTCTGC68355510.6685341203914275No Hit
TCCTCAAG67364840.6588451180410949No Hit
GAAGTCAG64959130.635316667755714No Hit
GTTCGACA61713130.6035699694311667No Hit
AGTTGGCT61306650.5995944925565636No Hit
GGGGGGGG61122340.5977918942915613No Hit
TACTCCTT57733870.5646518034499783ABI Solid3 GAPDH Reverse Primer (100% over 8bp)
AGGCTTAG51526540.5039425511668877No Hit
ATGTCAAT44243370.4327113124618991RNA PCR Primer, Index 15 (100% over 8bp)
TTCCGTCT39058970.38200657346196604TruSeq Adapter, Index 14 (100% over 8bp)
TAGCACAG38170490.37331701507398274No Hit
ACCTAGTA36354760.3555587179240043No Hit
TGGTTCAC30084180.29423086469543386No Hit
TCCAGAGA29829480.29173983448494023No Hit
TTCAGCAC26403610.2582339622147256No Hit
TGCAACCT22385740.21893818069986157No Hit
CTCTAGCT22356570.21865289074603314No Hit
TGGCGGTC21420600.2094988681857046No Hit
GAAGGACT20926110.2046626313235649No Hit
CGATCTCG13608580.13309534315633623Illumina Paired End PCR Primer 2 (100% over 8bp)
TTATGGCC13285300.12993358325665674No Hit
TGATCTCG12026590.11762308213278401Illumina PCR Primer Index 5 (100% over 8bp)
CCTGGCCA11744070.11485996530880029ABI Solid3 GAPDH Forward Primer (100% over 8bp)
TCTCAGTG10703620.10468410200880794No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)