FastQCFastQC Report
Sun 21 Jul 2024
HC5KGDRX5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC5KGDRX5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1022468531
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGG17963161117.568424411489296No Hit
CCTAAGAC493895994.830427294588296No Hit
TAGCGCTC492239754.814228849846137No Hit
ACTGAGCG489540684.787831264803982No Hit
CGATCAGT485252074.7458875778349014No Hit
ATGCGCAG353392943.4562720444253943No Hit
ACTCGCTA290750172.8436099614297077No Hit
TACGCTGC285050412.787864871706257No Hit
CGGAGCCT274868722.68828537667728No Hit
ATCTCAGG268351352.6245438550323463No Hit
GGAGCTAC245707772.403083934130389No Hit
AAGGACAC185050481.8098403460790717No Hit
TCCGTCTA183974601.7993179684470895No Hit
TTCACGCA172182251.683985812566783No Hit
TCTTCACA170995051.6723746972707565No Hit
GCTCATGA159281551.5578137142687278No Hit
GATCTACG148288971.4503035106123965No Hit
ACAGATTC146941411.4371240340892213No Hit
TGGCTTCA138919261.3586653846855654No Hit
AGCACCTC136890121.3388198839344034No Hit
GTAACGAG132893701.2997338888271368No Hit
GCGTAGTA131274461.2838973134127685No Hit
AGCCATGC121746601.1907124406159353No Hit
GCTACGCT116213971.1366019244263665No Hit
AATCCGTC112922271.1044082685807373No Hit
ACACGACC111517651.0906707308725965No Hit
TATCAGCA102776871.0051836989005583No Hit
TGCTCGTA91051280.8905044726506112No Hit
AGGCAGAA83822490.8198050840549537No Hit
ACGCTACT83287370.8145714755498915No Hit
CGTAGCGA82289570.8048127400020686No Hit
CAGAGAGG77195130.7549878324812717No Hit
CGAGGCTG72702510.7110488762807703No Hit
TGAGTACG72090670.7050649268344084No Hit
AAGAGGCA70959020.6939971045426727No Hit
TAGTCTCC68591970.6708467588035725No Hit
CGTACTAG68347590.6684566607947761No Hit
TAGGCATG67083150.6560901188263564No Hit
CGAGAGTT65664330.6422137015383607TruSeq Adapter, Index 13 (100% over 8bp)
CTCTCTAC64673440.6325225475325655No Hit
ATCATTCC64477840.6306095302213267No Hit
AACGCTGA63427860.6203404611187979No Hit
ACTCACTG62359430.6098909463649791No Hit
GACATAGT60756130.5942102681691238No Hit
CACTAGCT59629870.5831951614362203Illumina PCR Primer Index 10 (100% over 8bp)
GGACTCCT56023010.5479191613379836No Hit
TCCTGAGC54490010.5329260348649303No Hit
CTGCGTAG51808420.5066994086295258No Hit
GTCTGCTA50650940.49537896242598395No Hit
GAACCACA49975100.4887690768450653No Hit
GGTTGCAA49448080.483614688382033No Hit
ACTACGAC46200220.4518497987885751No Hit
GTAGAGGA44944560.43956912743361487No Hit
CGAGCGAC43675370.4271561292677085No Hit
TAAGGCGA41576260.40662630427693813No Hit
GCGACTAA32540050.3182498924262736No Hit
ACCGGTAA27270940.26671666827074214No Hit
GCATAAGG25248160.2469333699229517No Hit
AACCTCCA23946050.23419840585783272No Hit
GTCTATGA22797910.2229693072089277No Hit
ACAGTGGT22583150.22086890026740585No Hit
GATTCGGA20467760.2001798527724077No Hit
CATCTTCG19864630.19428108932185806No Hit
CATTACGG15539070.1519760220375917No Hit
CGTGTACA14172870.13861424161522679No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)