FastQCFastQC Report
Sun 21 Jul 2024
HC5KGDRX5_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC5KGDRX5_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1022468531
Sequences flagged as poor quality0
Sequence length301
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG19634328219.202867965821042No Hit
GGCTTGGTCATTTAGAGGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTG783425177.662095666003436No Hit
GGCTTGGTCATTTAGAGGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTG256610022.5097106876143065No Hit
GGCTTGGTCATTTAGAGGAAGGAGAAGTCGTAACAAGGTTTCCGTAGGTG212645782.079729336921764No Hit
GGCTTGGTCATTTAGAGGAAGTAGAAGTCGTAACAAGGTTTCCGTAGGTG200490591.9608485143686052No Hit
GGCTTGGTCATTTAGAGGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTG96375090.9425726765961465No Hit
GGCTTGGTCATTTAGAGGAAGTAAAAGTCATAACAAGGTTTCCGTAGGTG78035490.7632067651380853No Hit
GGCTTGGCATCGATGAAGAACGCAGCCGCTGTCTCTTATACACATCTCCG57926610.5665368492402042No Hit
GGCTTGATCATTTAGAGGAAGGAGAAGTCGTAACAAGGTTTCCGTAGGTG41123010.40219340501150347No Hit
GGCTTGGTCATTTAGAGGAAGAACGCAGCCGCTGTCTCTTATACACATCT26880300.2628961105894417No Hit
GGCTTGGTCATTTAGAGGAAGTAAAAGTCGTAACAAGGTTTCCGTTGGTG14500860.14182206650230883No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)