FastQCFastQC Report
Tue 7 Jun 2022
HC5JJDRX2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC5JJDRX2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences978097148
Sequences flagged as poor quality0
Sequence length10
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGTGGCG24608305425.159367298349387No Hit
TTCGTTGTGT17513904617.906099241585764No Hit
TGTGTGGTAC13942550814.25477093815225No Hit
TGCTGTGTAG13176472113.471537185179482No Hit
GTCTGTGCCG612797446.26520015167246No Hit
TGTGTGGAAC354291493.622252561767004No Hit
GTGTGTGGCG285656022.920528094618266No Hit
TTGGTTGTGT170862631.746888132220584No Hit
TGATGTGGGT155837381.593270978436592No Hit
GGGGGGGGGG147337741.5063712260205875No Hit
TGGTGTGTAG129493021.3239279990212178No Hit
ATCGTTGTGT115645211.1823489132594833No Hit
GACTGTGGCG111062291.1354934448699567No Hit
GACTGTGCCG98050961.002466474833234No Hit
AGCTGTGTAG81779200.8361050859540999No Hit
AACGTTGTGT66364930.6785106176385661No Hit
TCCTGTGTAG39150940.40027659910935554No Hit
TGTTGTGGGT36464420.3728097978259313No Hit
ACCTGTGTAG36244230.37055858995307084No Hit
TACGTTGTGT33304120.3404991014246368No Hit
GTCTGTCCCG21703660.2218967721598959No Hit
TTCGTTCTGT19329060.19761902015074684No Hit
TGCGTTGTGT18680990.19099319569838885No Hit
TGCTGTCTAG14597570.14924458199115412No Hit
GGCTGTGGCG13701340.1400815862515939No Hit
AGATGTGGGT12120350.12391764994697645No Hit
GTCTCTGCCG10943470.11188530732736582No Hit
GTCTGTCGCG10933400.11178235231905614No Hit
GGGGGTGGGG10711720.10951591078558182No Hit
GTCTCTCCCG10027580.10252130905916924No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)