FastQCFastQC Report
Tue 7 Jun 2022
HC5JJDRX2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC5JJDRX2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences978097148
Sequences flagged as poor quality0
Sequence length10
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTAGCATTT18924158719.347933626732136No Hit
CACGGTGTAT936425489.573951645956543No Hit
ACAGTAAGTA633374186.475575368920307No Hit
TGTTGGTTTT505014345.163232926633582No Hit
GTAGTCGTAA491252065.022528293886816No Hit
GGGGGGGGGG418745714.2812282078139745No Hit
ACAGTAAGTT324489183.317555732204221No Hit
ACAGTAACTA294564943.011612298454427No Hit
TCTTGGTTTT291237372.9775914447303964No Hit
GTTGTGGTAT276225872.8241148700292498No Hit
AGTGTTTGTT262552742.6843217009359894No Hit
CACGGTGAAT255832012.615609405702919No Hit
GTAGTCGTAT247647102.531927431813757No Hit
TCTACCATTT244118642.4958526921295143No Hit
CTGGGTGTTT179618651.8364090966554991No Hit
GTTGTGGTTT166015011.6973263886871082No Hit
GTAGTCGAAA149770831.5312469758883296No Hit
CAGGGTGTAT146419481.4969829970304749No Hit
TGTAGCATTT113253061.1578917312209562No Hit
TCTAGGATTT112620351.1514229463840537No Hit
ACTGTTTGTT111721741.1422356176832447No Hit
CTGGGTGTAT106711461.0910108491595356No Hit
TCTTGGATTT99655961.0188758877763338No Hit
GTAGACGAAA87791370.8975731110096233No Hit
ACTGTTAGTT85012600.8691631518794695No Hit
TCTTGCATTT77513390.7924917290526646No Hit
CTCGGTGTAT67194330.6869903479158289No Hit
GTTGTCGTAT57294520.5857753508141299No Hit
TCTTGCTTTT55900320.5715211430102237No Hit
TGTAGGATTT44721960.4572343359905186No Hit
TCTGTTTGTT44094880.45082311189808316No Hit
GTAGTGGTAT41563400.4249414292331624No Hit
GTAGTCGTTT35649680.36447995041081543No Hit
TGTTGGATTT33400390.34148335948322384No Hit
TGTGTTTGTT33019750.3375917215127183No Hit
ACTGTAAGTT32422990.3314904870778746No Hit
AGTGTTAGTT32379870.33104963107407No Hit
CTCGGTGTTT27063430.27669470313188155No Hit
AGAGTAAGTA26604480.27200242894481885No Hit
ACTGTATGTT26363900.2695427550720146No Hit
ACAGTTAGTT25818370.26396529274002134No Hit
GTAGACGTAA25735740.26312048913161745No Hit
GGGGGGGGTT24140560.24681147521350305No Hit
CACGGTGTTT22540300.2304505237142354No Hit
GGGGGGGGGT21664670.22149814100061155No Hit
ACAGTTTGTT19698750.20139870605164056No Hit
AGAGTAAGTT19674780.20115363837049038No Hit
AGTGTAAGTT19656880.2009706299644583No Hit
GCTAGCATTT15899980.16256033495764777No Hit
GTAGTGGTAA15610140.15959703013058985No Hit
ACTGTAAGTA13224300.13520436111117257No Hit
ACAGTATGTT13161080.1345580040480805No Hit
ACAGTTAGTA13134940.1342907504316739No Hit
TCAGTAAGTA12908460.13197523401836972No Hit
GGGGGGGGTG12529610.12810189688846735No Hit
TCTAGCTTTT12016880.12285977956864465No Hit
GTTGTCGTAA10477700.10712330591521162No Hit
GCAGTAAGTA10470600.10705071598879665No Hit
GGTAGCATTT10076520.10302166835476756No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)