FastQCFastQC Report
Tue 7 Jun 2022
HC5JJDRX2_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC5JJDRX2_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences978097148
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCTCTCTACACGACGCTCTTCCGATC25940280.2652116924483661Illumina Single End PCR Primer 1 (100% over 21bp)
AAGGCCCCTACACGACGCTCTTCCGATC14721840.15051511018208183Illumina Single End PCR Primer 1 (95% over 24bp)
GTGCCTTCTACACGACGCTCTTCCGATC10191930.10420161249667605Illumina Single End PCR Primer 1 (95% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)