FastQCFastQC Report
Sat 11 Jun 2022
HC5JHDRX2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC5JHDRX2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870639448
Sequences flagged as poor quality0
Sequence length10
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTCCCG16933500319.449498111875123No Hit
GACTGTCCCG15845487018.199826617550645No Hit
AACGTACTGT9239869510.61273931617213No Hit
TTGGTTCTGT652420517.493578558824962No Hit
GTGTCTCCCG500068565.743692881694467No Hit
GACTCTCCCG375996214.318621340483989No Hit
GTGTGTGCCG202512042.3260149820365132No Hit
AACGAACTGT193888492.2269665180620213No Hit
GGGGGGGGGG187437692.152873849566256No Hit
ATGGTACTGT182581312.0970943875724775No Hit
TTGGAACTGT168249611.9324831925143826No Hit
GTCTCTCCCG161550181.8555348068722013No Hit
ATGGTTCTGT160307561.8412623086198594No Hit
GTCTGTCCCG147509881.6942705770896793No Hit
ATGGAACTGT143522511.6484723995644177RNA PCR Primer, Index 14 (100% over 10bp)
GACTGTGCCG134360171.5432354955733638No Hit
TTCGAACTGT104815081.2038861809073462No Hit
GAGTGTCCCG82814910.951196390081328No Hit
AACGTTGTGT76153630.8746861881222731No Hit
AGATGTCGGT75504110.8672259242726158No Hit
TTGGTTGTGT73187320.8406157126020781No Hit
TGTTGTCGGT63939850.734401021535151No Hit
GTGTCTGCCG63453450.7288143231479215No Hit
TTGGTACTGT57901560.66504636486446No Hit
GTCTGTGCCG56798380.6523754480741148No Hit
TTCGTACTGT53257160.611701665050215No Hit
ATGGTTGTGT52327520.6010239958711359No Hit
TTCGTTGTGT36955070.42445894319275096No Hit
TTCGTTCTGT36198280.41576659641546587No Hit
AGTTGTCGGT33568100.38555684648922545No Hit
GAGTCTCCCG26546060.3049030234154977No Hit
ATGGAAGTGT22239890.2554431693979481No Hit
TACGAACTGT21265180.24424783472480563No Hit
TGTTCTCGGT18288270.21005560960982322No Hit
TTGGAAGTGT17456800.20050550247982793No Hit
AGATCTCGGT16089340.18479911560359255Illumina Single End PCR Primer 1 (100% over 10bp)
GTCTCTGCCG13772730.15819097137900417No Hit
AAGGTACTGT12732710.14624549840061923No Hit
AGTTCTCGGT12374810.14213472670491722No Hit
AACGTTCTGT11166400.1282551580410356No Hit
GAGTGTGCCG11041540.12682103970092565No Hit
TTCGAAGTGT8944090.1027301257776227No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)