FastQCFastQC Report
Sat 11 Jun 2022
HC5JHDRX2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC5JHDRX2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870639448
Sequences flagged as poor quality0
Sequence length10
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCATTT43890863950.41221598771389No Hit
ACAGTAACTA21674471524.894887946772656No Hit
GGGGGGGGGG537022206.168135400177732No Hit
ACAGTAACTT498595815.7267771537960455No Hit
TCTTCCATTT476306545.470766815059361No Hit
TCAGTAACTA122639581.4086150160290003No Hit
ACTGTAACTT120434131.3832836345361645No Hit
TCAGTAACTT29109800.3343496560702588No Hit
TCTACCATTA15680920.18010808074480905No Hit
GCTACCATTT15564850.1787749226818861No Hit
TCTTCGATTT15422520.1771401472265934No Hit
GGTACCATTT15302420.17576070134602953No Hit
GGGGGGGGTG15025110.1725755711450373No Hit
TCTGTAACTT12283530.1410863018924454No Hit
GCAGTAACTA9286430.10666217825682532No Hit
TCTACATTTA8947920.1027741164330978No Hit
ACAGTTACTT8803870.10111958538317918No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)