FastQCFastQC Report
Sat 11 Jun 2022
HC5JHDRX2_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC5JHDRX2_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870639448
Sequences flagged as poor quality0
Sequence length28
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCTCTCTACACGACGCTCTTCCGATC38255920.439400260209666Illumina Single End PCR Primer 1 (100% over 21bp)
AAGGCCCCTACACGACGCTCTTCCGATC24937100.28642281322405694Illumina Single End PCR Primer 1 (95% over 24bp)
GTGCCTTCTACACGACGCTCTTCCGATC16164170.18565859882792723Illumina Single End PCR Primer 1 (95% over 22bp)
GGTTGCTCTACACGACGCTCTTCCGATC14664320.16843160545603947Illumina Single End PCR Primer 1 (100% over 21bp)
GTTCCTTCTACACGACGCTCTTCCGATC13660260.15689916223506564Illumina Single End PCR Primer 1 (100% over 21bp)
AACCGCTCTACACGACGCTCTTCCGATC13542110.15554211368561835Illumina Single End PCR Primer 1 (100% over 21bp)
GTCCTCCCTACACGACGCTCTTCCGATC12738800.14631544698856788Illumina Single End PCR Primer 1 (100% over 24bp)
GGTGCTTCTACACGACGCTCTTCCGATC12694500.14580662556884283Illumina Single End PCR Primer 1 (100% over 21bp)
TGTGGCTCTACACGACGCTCTTCCGATC12416240.14261058384756303Illumina Single End PCR Primer 1 (100% over 21bp)
ACGCCTTCTACACGACGCTCTTCCGATC11936470.13710003638612983Illumina Single End PCR Primer 1 (100% over 21bp)
AAAGGCTCTACACGACGCTCTTCCGATC10445870.11997928676441043Illumina Single End PCR Primer 1 (100% over 21bp)
GGTTGATCCTGCCAGTAGTTATATGCTT10401600.11947080991901024No Hit
ACGTCCTCTACACGACGCTCTTCCGATC9906860.11378831986946679Illumina Single End PCR Primer 1 (100% over 21bp)
TAACCTATTCTCAAACTTTAAATGGGTA9743660.11191383554217267No Hit
GTCCTCCTACACGACGCTCTTCCGATCT9637730.11069714360105586Illumina Single End PCR Primer 1 (100% over 23bp)
GAGCCTTCTACACGACGCTCTTCCGATC8844530.10158659845148667Illumina Single End PCR Primer 1 (95% over 22bp)
GACGCCTCTACACGACGCTCTTCCGATC8774810.10078580772048822Illumina Single End PCR Primer 1 (100% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)