FastQCFastQC Report
Tue 7 Jun 2022
HC5HJDRX2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC5HJDRX2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences789989673
Sequences flagged as poor quality0
Sequence length8
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTAAGA12297469215.566620198084538No Hit
CCTACCAT11830861914.975970325120947No Hit
AAACGGCG10601583513.419901376356346No Hit
GGCGTTTC10035160512.702900864376234No Hit
CAATCCCT8309311810.518253698741704No Hit
TGTGTATA703043168.899396840596422No Hit
ACGAGTAG643035998.139802480683821No Hit
GTCCAGGC512176026.4833255105095535No Hit
GGGGGGGG141051031.7854794160075051No Hit
TTCTAATA11021560.13951524148594788No Hit
AATAAAAA10882470.13775458555899375No Hit
CCAAAAAA10020190.1268395061665572No Hit
AAAAAACA8339230.10556125333045967No Hit
GGTGTATA8221270.1040680692543686No Hit
GTGTAAGA8075010.10221665264730619No Hit
AAACTTAC8025550.1015905685136722No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)