FastQCFastQC Report
Tue 7 Jun 2022
HC5HJDRX2_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC5HJDRX2_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences789989673
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGCC13050200.16519456451173128No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCCCTATCTCGTATGCCGT12166440.15400758283076948No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTACCATATCTCGTATGCCGT9629230.12189058071395828No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGTATAATCTCGTATGCCGT9580330.1212715852805838No Hit
CTTATACACATCTCCGAGCCCACGAGACACGAGTAGATCTCGTATGCCGT9284570.11752773887210041RNA PCR Primer, Index 23 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGGCGTTTCATCTCGTATGCCGT8870530.1122866577016634No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)