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        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.9

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        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2024-06-14, 15:32 based on data in: /vast/gencore/GENEFLOW/work/c0/5526c144b9f6ac0d25166c07244119/2


        General Statistics

        Showing 258/258 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        HC5FNDRX5_l02_n01_AtbZIP11_An1AmpPoolHmB-B_ak1
        22.8%
        40%
        2.6
        HC5FNDRX5_l02_n01_AtbZIP11_An1AmpPoolHmB-B_ak2
        22.7%
        40%
        2.6
        HC5FNDRX5_l02_n01_AtbZIP11_C24AmpPoolHmB-B_ak1
        24.3%
        39%
        3.3
        HC5FNDRX5_l02_n01_AtbZIP11_C24AmpPoolHmB-B_ak2
        24.4%
        39%
        3.3
        HC5FNDRX5_l02_n01_AtbZIP11_ColAmpPoolHmB-B_ak1
        23.0%
        40%
        3.2
        HC5FNDRX5_l02_n01_AtbZIP11_ColAmpPoolHmB-B_ak2
        23.5%
        40%
        3.3
        HC5FNDRX5_l02_n01_AtbZIP11_CviAmpPoolHmB-B_ak1
        23.2%
        39%
        2.7
        HC5FNDRX5_l02_n01_AtbZIP11_CviAmpPoolHmB-B_ak2
        23.5%
        39%
        2.8
        HC5FNDRX5_l02_n01_AtbZIP11_EriAmpPoolHmB-B_ak1
        18.2%
        39%
        2.4
        HC5FNDRX5_l02_n01_AtbZIP11_EriAmpPoolHmB-B_ak2
        18.4%
        39%
        2.4
        HC5FNDRX5_l02_n01_AtbZIP11_KyoAmpPoolHmB-B_ak1
        23.0%
        40%
        2.4
        HC5FNDRX5_l02_n01_AtbZIP11_KyoAmpPoolHmB-B_ak2
        23.0%
        40%
        2.4
        HC5FNDRX5_l02_n01_AtbZIP11_LerAmpPoolHmB-B_ak1
        23.3%
        40%
        2.7
        HC5FNDRX5_l02_n01_AtbZIP11_LerAmpPoolHmB-B_ak2
        23.6%
        40%
        2.7
        HC5FNDRX5_l02_n01_AtbZIP11_ShaAmpPoolHmB-B_ak1
        21.3%
        39%
        3.0
        HC5FNDRX5_l02_n01_AtbZIP11_ShaAmpPoolHmB-B_ak2
        21.7%
        39%
        3.0
        HC5FNDRX5_l02_n01_AtbZIP1_An1AmpPoolHmB-B_ak1
        24.6%
        38%
        1.9
        HC5FNDRX5_l02_n01_AtbZIP1_An1AmpPoolHmB-B_ak2
        22.3%
        38%
        2.1
        HC5FNDRX5_l02_n01_AtbZIP1_C24AmpPoolHmB-B_ak1
        23.8%
        37%
        2.0
        HC5FNDRX5_l02_n01_AtbZIP1_C24AmpPoolHmB-B_ak2
        22.5%
        38%
        2.4
        HC5FNDRX5_l02_n01_AtbZIP1_ColAmpPoolHmB-B_ak1
        24.8%
        37%
        2.4
        HC5FNDRX5_l02_n01_AtbZIP1_ColAmpPoolHmB-B_ak2
        22.6%
        38%
        2.6
        HC5FNDRX5_l02_n01_AtbZIP1_CviAmpPoolHmB-B_ak1
        24.6%
        37%
        2.0
        HC5FNDRX5_l02_n01_AtbZIP1_CviAmpPoolHmB-B_ak2
        22.8%
        38%
        2.1
        HC5FNDRX5_l02_n01_AtbZIP1_EriAmpPoolHmB-B_ak1
        20.3%
        37%
        1.7
        HC5FNDRX5_l02_n01_AtbZIP1_EriAmpPoolHmB-B_ak2
        17.6%
        37%
        1.8
        HC5FNDRX5_l02_n01_AtbZIP1_KyoAmpPoolHmB-B_ak1
        24.1%
        38%
        1.8
        HC5FNDRX5_l02_n01_AtbZIP1_KyoAmpPoolHmB-B_ak2
        21.5%
        38%
        1.9
        HC5FNDRX5_l02_n01_AtbZIP1_LerAmpPoolHmB-B_ak1
        25.2%
        38%
        2.0
        HC5FNDRX5_l02_n01_AtbZIP1_LerAmpPoolHmB-B_ak2
        22.6%
        38%
        2.1
        HC5FNDRX5_l02_n01_AtbZIP1_ShaAmpPoolHmB-B_ak1
        21.9%
        37%
        2.0
        HC5FNDRX5_l02_n01_AtbZIP1_ShaAmpPoolHmB-B_ak2
        20.0%
        38%
        2.2
        HC5FNDRX5_l02_n01_AtbZIP26_An1AmpPoolHmB-B_ak2
        31.8%
        38%
        1.7
        HC5FNDRX5_l02_n01_AtbZIP26_C24AmpPoolHmB-B_ak2
        28.8%
        37%
        1.3
        HC5FNDRX5_l02_n01_AtbZIP26_ColAmpPoolHmB-B_ak2
        30.6%
        38%
        1.9
        HC5FNDRX5_l02_n01_AtbZIP26_CviAmpPoolHmB-B_ak2
        31.1%
        38%
        1.6
        HC5FNDRX5_l02_n01_AtbZIP26_EriAmpPoolHmB-B_ak2
        26.9%
        37%
        1.3
        HC5FNDRX5_l02_n01_AtbZIP26_KyoAmpPoolHmB-B_ak2
        30.3%
        38%
        1.5
        HC5FNDRX5_l02_n01_AtbZIP26_LerAmpPoolHmB-B_ak2
        30.7%
        38%
        1.6
        HC5FNDRX5_l02_n01_AtbZIP26_ShaAmpPoolHmB-B_ak2
        28.9%
        38%
        1.5
        HC5FNDRX5_l02_n01_AtbZIP3_An1AmpPoolHmB-B_ak1
        23.6%
        39%
        2.2
        HC5FNDRX5_l02_n01_AtbZIP3_An1AmpPoolHmB-B_ak2
        23.5%
        38%
        2.5
        HC5FNDRX5_l02_n01_AtbZIP3_C24AmpPoolHmB-B_ak1
        23.4%
        38%
        2.5
        HC5FNDRX5_l02_n01_AtbZIP3_C24AmpPoolHmB-B_ak2
        23.5%
        37%
        2.9
        HC5FNDRX5_l02_n01_AtbZIP3_ColAmpPoolHmB-B_ak1
        23.2%
        38%
        2.7
        HC5FNDRX5_l02_n01_AtbZIP3_ColAmpPoolHmB-B_ak2
        23.6%
        38%
        3.1
        HC5FNDRX5_l02_n01_AtbZIP3_CviAmpPoolHmB-B_ak1
        23.8%
        38%
        2.3
        HC5FNDRX5_l02_n01_AtbZIP3_CviAmpPoolHmB-B_ak2
        23.8%
        38%
        2.6
        HC5FNDRX5_l02_n01_AtbZIP3_EriAmpPoolHmB-B_ak1
        18.7%
        38%
        2.0
        HC5FNDRX5_l02_n01_AtbZIP3_EriAmpPoolHmB-B_ak2
        18.6%
        37%
        2.3
        HC5FNDRX5_l02_n01_AtbZIP3_KyoAmpPoolHmB-B_ak1
        22.6%
        38%
        2.1
        HC5FNDRX5_l02_n01_AtbZIP3_KyoAmpPoolHmB-B_ak2
        22.7%
        38%
        2.4
        HC5FNDRX5_l02_n01_AtbZIP3_LerAmpPoolHmB-B_ak1
        23.7%
        38%
        2.3
        HC5FNDRX5_l02_n01_AtbZIP3_LerAmpPoolHmB-B_ak2
        23.6%
        38%
        2.6
        HC5FNDRX5_l02_n01_AtbZIP3_ShaAmpPoolHmB-B_ak1
        21.1%
        38%
        2.4
        HC5FNDRX5_l02_n01_AtbZIP3_ShaAmpPoolHmB-B_ak2
        21.3%
        38%
        2.8
        HC5FNDRX5_l02_n01_AtbZIP41_An1AmpPoolHmB-B_ak1
        29.4%
        39%
        2.2
        HC5FNDRX5_l02_n01_AtbZIP41_An1AmpPoolHmB-B_ak2
        26.2%
        39%
        2.3
        HC5FNDRX5_l02_n01_AtbZIP41_C24AmpPoolHmB-B_ak1
        28.2%
        38%
        1.9
        HC5FNDRX5_l02_n01_AtbZIP41_C24AmpPoolHmB-B_ak2
        25.5%
        38%
        2.0
        HC5FNDRX5_l02_n01_AtbZIP41_ColAmpPoolHmB-B_ak1
        29.0%
        39%
        2.6
        HC5FNDRX5_l02_n01_AtbZIP41_ColAmpPoolHmB-B_ak2
        25.9%
        38%
        2.7
        HC5FNDRX5_l02_n01_AtbZIP41_CviAmpPoolHmB-B_ak1
        28.8%
        38%
        2.1
        HC5FNDRX5_l02_n01_AtbZIP41_CviAmpPoolHmB-B_ak2
        25.9%
        38%
        2.3
        HC5FNDRX5_l02_n01_AtbZIP41_EriAmpPoolHmB-B_ak1
        25.8%
        38%
        1.9
        HC5FNDRX5_l02_n01_AtbZIP41_EriAmpPoolHmB-B_ak2
        21.8%
        38%
        1.9
        HC5FNDRX5_l02_n01_AtbZIP41_KyoAmpPoolHmB-B_ak1
        28.5%
        38%
        1.9
        HC5FNDRX5_l02_n01_AtbZIP41_KyoAmpPoolHmB-B_ak2
        25.2%
        39%
        2.0
        HC5FNDRX5_l02_n01_AtbZIP41_LerAmpPoolHmB-B_ak1
        29.3%
        39%
        2.1
        HC5FNDRX5_l02_n01_AtbZIP41_LerAmpPoolHmB-B_ak2
        26.0%
        39%
        2.2
        HC5FNDRX5_l02_n01_AtbZIP41_ShaAmpPoolHmB-B_ak1
        27.4%
        38%
        2.1
        HC5FNDRX5_l02_n01_AtbZIP41_ShaAmpPoolHmB-B_ak2
        24.1%
        38%
        2.2
        HC5FNDRX5_l02_n01_AtbZIP44_An1AmpPoolHmB-B_ak1
        27.2%
        41%
        3.1
        HC5FNDRX5_l02_n01_AtbZIP44_An1AmpPoolHmB-B_ak2
        26.4%
        40%
        2.6
        HC5FNDRX5_l02_n01_AtbZIP44_C24AmpPoolHmB-B_ak1
        28.1%
        40%
        4.1
        HC5FNDRX5_l02_n01_AtbZIP44_C24AmpPoolHmB-B_ak2
        27.1%
        40%
        3.5
        HC5FNDRX5_l02_n01_AtbZIP44_ColAmpPoolHmB-B_ak1
        27.1%
        40%
        4.2
        HC5FNDRX5_l02_n01_AtbZIP44_ColAmpPoolHmB-B_ak2
        26.2%
        40%
        3.6
        HC5FNDRX5_l02_n01_AtbZIP44_CviAmpPoolHmB-B_ak1
        28.2%
        40%
        3.8
        HC5FNDRX5_l02_n01_AtbZIP44_CviAmpPoolHmB-B_ak2
        27.6%
        40%
        3.3
        HC5FNDRX5_l02_n01_AtbZIP44_EriAmpPoolHmB-B_ak1
        22.1%
        40%
        3.3
        HC5FNDRX5_l02_n01_AtbZIP44_EriAmpPoolHmB-B_ak2
        21.2%
        39%
        2.7
        HC5FNDRX5_l02_n01_AtbZIP44_KyoAmpPoolHmB-B_ak1
        27.1%
        40%
        3.0
        HC5FNDRX5_l02_n01_AtbZIP44_KyoAmpPoolHmB-B_ak2
        26.0%
        40%
        2.5
        HC5FNDRX5_l02_n01_AtbZIP44_LerAmpPoolHmB-B_ak1
        27.2%
        40%
        3.3
        HC5FNDRX5_l02_n01_AtbZIP44_LerAmpPoolHmB-B_ak2
        26.3%
        40%
        2.7
        HC5FNDRX5_l02_n01_AtbZIP44_ShaAmpPoolHmB-B_ak1
        25.9%
        40%
        4.0
        HC5FNDRX5_l02_n01_AtbZIP44_ShaAmpPoolHmB-B_ak2
        24.9%
        40%
        3.4
        HC5FNDRX5_l02_n01_AtbZIP50_An1AmpPoolHmB-B_ak1
        25.7%
        40%
        2.2
        HC5FNDRX5_l02_n01_AtbZIP50_An1AmpPoolHmB-B_ak2
        23.9%
        40%
        2.0
        HC5FNDRX5_l02_n01_AtbZIP50_C24AmpPoolHmB-B_ak1
        24.8%
        38%
        2.4
        HC5FNDRX5_l02_n01_AtbZIP50_C24AmpPoolHmB-B_ak2
        23.7%
        39%
        2.5
        HC5FNDRX5_l02_n01_AtbZIP50_ColAmpPoolHmB-B_ak1
        25.1%
        39%
        2.5
        HC5FNDRX5_l02_n01_AtbZIP50_ColAmpPoolHmB-B_ak2
        23.3%
        40%
        2.4
        HC5FNDRX5_l02_n01_AtbZIP50_CviAmpPoolHmB-B_ak1
        25.2%
        39%
        2.2
        HC5FNDRX5_l02_n01_AtbZIP50_CviAmpPoolHmB-B_ak2
        23.6%
        39%
        2.1
        HC5FNDRX5_l02_n01_AtbZIP50_EriAmpPoolHmB-B_ak1
        21.1%
        39%
        2.0
        HC5FNDRX5_l02_n01_AtbZIP50_EriAmpPoolHmB-B_ak2
        19.0%
        39%
        1.9
        HC5FNDRX5_l02_n01_AtbZIP50_KyoAmpPoolHmB-B_ak1
        24.7%
        39%
        2.1
        HC5FNDRX5_l02_n01_AtbZIP50_KyoAmpPoolHmB-B_ak2
        22.6%
        39%
        1.9
        HC5FNDRX5_l02_n01_AtbZIP50_LerAmpPoolHmB-B_ak1
        26.0%
        39%
        2.3
        HC5FNDRX5_l02_n01_AtbZIP50_LerAmpPoolHmB-B_ak2
        24.2%
        40%
        2.1
        HC5FNDRX5_l02_n01_AtbZIP50_ShaAmpPoolHmB-B_ak1
        22.9%
        39%
        2.4
        HC5FNDRX5_l02_n01_AtbZIP50_ShaAmpPoolHmB-B_ak2
        21.2%
        39%
        2.3
        HC5FNDRX5_l02_n01_AtbZIP66_An1AmpPoolHmB-B_ak1
        21.6%
        38%
        2.5
        HC5FNDRX5_l02_n01_AtbZIP66_An1AmpPoolHmB-B_ak2
        22.6%
        38%
        3.0
        HC5FNDRX5_l02_n01_AtbZIP66_C24AmpPoolHmB-B_ak1
        21.6%
        37%
        2.8
        HC5FNDRX5_l02_n01_AtbZIP66_C24AmpPoolHmB-B_ak2
        22.7%
        37%
        3.3
        HC5FNDRX5_l02_n01_AtbZIP66_ColAmpPoolHmB-B_ak1
        21.9%
        37%
        3.2
        HC5FNDRX5_l02_n01_AtbZIP66_ColAmpPoolHmB-B_ak2
        23.3%
        38%
        3.8
        HC5FNDRX5_l02_n01_AtbZIP66_CviAmpPoolHmB-B_ak1
        22.1%
        37%
        2.5
        HC5FNDRX5_l02_n01_AtbZIP66_CviAmpPoolHmB-B_ak2
        23.2%
        38%
        3.0
        HC5FNDRX5_l02_n01_AtbZIP66_EriAmpPoolHmB-B_ak1
        16.3%
        37%
        2.1
        HC5FNDRX5_l02_n01_AtbZIP66_EriAmpPoolHmB-B_ak2
        17.5%
        37%
        2.6
        HC5FNDRX5_l02_n01_AtbZIP66_KyoAmpPoolHmB-B_ak1
        20.8%
        37%
        2.4
        HC5FNDRX5_l02_n01_AtbZIP66_KyoAmpPoolHmB-B_ak2
        21.9%
        38%
        2.9
        HC5FNDRX5_l02_n01_AtbZIP66_LerAmpPoolHmB-B_ak1
        21.9%
        38%
        2.5
        HC5FNDRX5_l02_n01_AtbZIP66_LerAmpPoolHmB-B_ak2
        23.3%
        38%
        3.1
        HC5FNDRX5_l02_n01_AtbZIP66_ShaAmpPoolHmB-B_ak1
        18.6%
        37%
        2.6
        HC5FNDRX5_l02_n01_AtbZIP66_ShaAmpPoolHmB-B_ak2
        19.5%
        37%
        3.2
        HC5FNDRX5_l02_n01_pIXHALO_An1AmpPoolHm-B_ak2
        31.3%
        37%
        0.9
        HC5FNDRX5_l02_n01_pIXHALO_C24AmpPoolHm-B_ak2
        29.7%
        36%
        0.7
        HC5FNDRX5_l02_n01_pIXHALO_ColAmpPoolHm-B_ak2
        30.6%
        37%
        1.1
        HC5FNDRX5_l02_n01_pIXHALO_CviAmpPoolHm-B_ak2
        30.9%
        37%
        0.9
        HC5FNDRX5_l02_n01_pIXHALO_EriAmpPoolHm-B_ak2
        28.8%
        36%
        0.7
        HC5FNDRX5_l02_n01_pIXHALO_KyoAmpPoolHm-B_ak2
        30.5%
        37%
        0.8
        HC5FNDRX5_l02_n01_pIXHALO_LerAmpPoolHm-B_ak2
        30.7%
        37%
        0.9
        HC5FNDRX5_l02_n01_pIXHALO_ShaAmpPoolHm-B_ak2
        30.0%
        36%
        0.8
        HC5FNDRX5_l02_n01_undetermined
        63.7%
        41%
        36.6
        HC5FNDRX5_l02_n02_AtbZIP11_An1AmpPoolHmB-B_ak1
        22.2%
        40%
        2.6
        HC5FNDRX5_l02_n02_AtbZIP11_An1AmpPoolHmB-B_ak2
        22.3%
        40%
        2.6
        HC5FNDRX5_l02_n02_AtbZIP11_C24AmpPoolHmB-B_ak1
        23.9%
        39%
        3.3
        HC5FNDRX5_l02_n02_AtbZIP11_C24AmpPoolHmB-B_ak2
        24.1%
        39%
        3.3
        HC5FNDRX5_l02_n02_AtbZIP11_ColAmpPoolHmB-B_ak1
        22.3%
        39%
        3.2
        HC5FNDRX5_l02_n02_AtbZIP11_ColAmpPoolHmB-B_ak2
        23.1%
        39%
        3.3
        HC5FNDRX5_l02_n02_AtbZIP11_CviAmpPoolHmB-B_ak1
        22.8%
        39%
        2.7
        HC5FNDRX5_l02_n02_AtbZIP11_CviAmpPoolHmB-B_ak2
        23.2%
        39%
        2.8
        HC5FNDRX5_l02_n02_AtbZIP11_EriAmpPoolHmB-B_ak1
        17.7%
        39%
        2.4
        HC5FNDRX5_l02_n02_AtbZIP11_EriAmpPoolHmB-B_ak2
        18.0%
        39%
        2.4
        HC5FNDRX5_l02_n02_AtbZIP11_KyoAmpPoolHmB-B_ak1
        22.1%
        40%
        2.4
        HC5FNDRX5_l02_n02_AtbZIP11_KyoAmpPoolHmB-B_ak2
        22.5%
        40%
        2.4
        HC5FNDRX5_l02_n02_AtbZIP11_LerAmpPoolHmB-B_ak1
        22.7%
        40%
        2.7
        HC5FNDRX5_l02_n02_AtbZIP11_LerAmpPoolHmB-B_ak2
        23.1%
        40%
        2.7
        HC5FNDRX5_l02_n02_AtbZIP11_ShaAmpPoolHmB-B_ak1
        20.6%
        39%
        3.0
        HC5FNDRX5_l02_n02_AtbZIP11_ShaAmpPoolHmB-B_ak2
        21.2%
        39%
        3.0
        HC5FNDRX5_l02_n02_AtbZIP1_An1AmpPoolHmB-B_ak1
        23.7%
        38%
        1.9
        HC5FNDRX5_l02_n02_AtbZIP1_An1AmpPoolHmB-B_ak2
        21.6%
        38%
        2.1
        HC5FNDRX5_l02_n02_AtbZIP1_C24AmpPoolHmB-B_ak1
        23.4%
        37%
        2.0
        HC5FNDRX5_l02_n02_AtbZIP1_C24AmpPoolHmB-B_ak2
        21.9%
        38%
        2.4
        HC5FNDRX5_l02_n02_AtbZIP1_ColAmpPoolHmB-B_ak1
        24.1%
        37%
        2.4
        HC5FNDRX5_l02_n02_AtbZIP1_ColAmpPoolHmB-B_ak2
        22.1%
        38%
        2.6
        HC5FNDRX5_l02_n02_AtbZIP1_CviAmpPoolHmB-B_ak1
        23.9%
        37%
        2.0
        HC5FNDRX5_l02_n02_AtbZIP1_CviAmpPoolHmB-B_ak2
        22.4%
        38%
        2.1
        HC5FNDRX5_l02_n02_AtbZIP1_EriAmpPoolHmB-B_ak1
        19.8%
        37%
        1.7
        HC5FNDRX5_l02_n02_AtbZIP1_EriAmpPoolHmB-B_ak2
        17.1%
        37%
        1.8
        HC5FNDRX5_l02_n02_AtbZIP1_KyoAmpPoolHmB-B_ak1
        23.3%
        37%
        1.8
        HC5FNDRX5_l02_n02_AtbZIP1_KyoAmpPoolHmB-B_ak2
        20.9%
        38%
        1.9
        HC5FNDRX5_l02_n02_AtbZIP1_LerAmpPoolHmB-B_ak1
        24.4%
        38%
        2.0
        HC5FNDRX5_l02_n02_AtbZIP1_LerAmpPoolHmB-B_ak2
        22.0%
        38%
        2.1
        HC5FNDRX5_l02_n02_AtbZIP1_ShaAmpPoolHmB-B_ak1
        21.1%
        37%
        2.0
        HC5FNDRX5_l02_n02_AtbZIP1_ShaAmpPoolHmB-B_ak2
        19.3%
        38%
        2.2
        HC5FNDRX5_l02_n02_AtbZIP26_An1AmpPoolHmB-B_ak2
        29.9%
        38%
        1.7
        HC5FNDRX5_l02_n02_AtbZIP26_C24AmpPoolHmB-B_ak2
        27.5%
        37%
        1.3
        HC5FNDRX5_l02_n02_AtbZIP26_ColAmpPoolHmB-B_ak2
        29.1%
        38%
        1.9
        HC5FNDRX5_l02_n02_AtbZIP26_CviAmpPoolHmB-B_ak2
        29.6%
        38%
        1.6
        HC5FNDRX5_l02_n02_AtbZIP26_EriAmpPoolHmB-B_ak2
        25.6%
        37%
        1.3
        HC5FNDRX5_l02_n02_AtbZIP26_KyoAmpPoolHmB-B_ak2
        28.6%
        38%
        1.5
        HC5FNDRX5_l02_n02_AtbZIP26_LerAmpPoolHmB-B_ak2
        29.3%
        38%
        1.6
        HC5FNDRX5_l02_n02_AtbZIP26_ShaAmpPoolHmB-B_ak2
        27.4%
        37%
        1.5
        HC5FNDRX5_l02_n02_AtbZIP3_An1AmpPoolHmB-B_ak1
        22.8%
        39%
        2.2
        HC5FNDRX5_l02_n02_AtbZIP3_An1AmpPoolHmB-B_ak2
        23.0%
        38%
        2.5
        HC5FNDRX5_l02_n02_AtbZIP3_C24AmpPoolHmB-B_ak1
        22.9%
        38%
        2.5
        HC5FNDRX5_l02_n02_AtbZIP3_C24AmpPoolHmB-B_ak2
        23.2%
        37%
        2.9
        HC5FNDRX5_l02_n02_AtbZIP3_ColAmpPoolHmB-B_ak1
        22.5%
        38%
        2.7
        HC5FNDRX5_l02_n02_AtbZIP3_ColAmpPoolHmB-B_ak2
        23.0%
        38%
        3.1
        HC5FNDRX5_l02_n02_AtbZIP3_CviAmpPoolHmB-B_ak1
        23.2%
        38%
        2.3
        HC5FNDRX5_l02_n02_AtbZIP3_CviAmpPoolHmB-B_ak2
        23.4%
        38%
        2.6
        HC5FNDRX5_l02_n02_AtbZIP3_EriAmpPoolHmB-B_ak1
        18.1%
        38%
        2.0
        HC5FNDRX5_l02_n02_AtbZIP3_EriAmpPoolHmB-B_ak2
        18.2%
        37%
        2.3
        HC5FNDRX5_l02_n02_AtbZIP3_KyoAmpPoolHmB-B_ak1
        21.9%
        38%
        2.1
        HC5FNDRX5_l02_n02_AtbZIP3_KyoAmpPoolHmB-B_ak2
        22.0%
        38%
        2.4
        HC5FNDRX5_l02_n02_AtbZIP3_LerAmpPoolHmB-B_ak1
        22.9%
        38%
        2.3
        HC5FNDRX5_l02_n02_AtbZIP3_LerAmpPoolHmB-B_ak2
        23.0%
        38%
        2.6
        HC5FNDRX5_l02_n02_AtbZIP3_ShaAmpPoolHmB-B_ak1
        20.4%
        38%
        2.4
        HC5FNDRX5_l02_n02_AtbZIP3_ShaAmpPoolHmB-B_ak2
        20.6%
        38%
        2.8
        HC5FNDRX5_l02_n02_AtbZIP41_An1AmpPoolHmB-B_ak1
        27.8%
        39%
        2.2
        HC5FNDRX5_l02_n02_AtbZIP41_An1AmpPoolHmB-B_ak2
        25.0%
        39%
        2.3
        HC5FNDRX5_l02_n02_AtbZIP41_C24AmpPoolHmB-B_ak1
        27.1%
        38%
        1.9
        HC5FNDRX5_l02_n02_AtbZIP41_C24AmpPoolHmB-B_ak2
        24.7%
        38%
        2.0
        HC5FNDRX5_l02_n02_AtbZIP41_ColAmpPoolHmB-B_ak1
        27.6%
        38%
        2.6
        HC5FNDRX5_l02_n02_AtbZIP41_ColAmpPoolHmB-B_ak2
        24.9%
        38%
        2.7
        HC5FNDRX5_l02_n02_AtbZIP41_CviAmpPoolHmB-B_ak1
        27.4%
        38%
        2.1
        HC5FNDRX5_l02_n02_AtbZIP41_CviAmpPoolHmB-B_ak2
        24.8%
        38%
        2.3
        HC5FNDRX5_l02_n02_AtbZIP41_EriAmpPoolHmB-B_ak1
        24.7%
        38%
        1.9
        HC5FNDRX5_l02_n02_AtbZIP41_EriAmpPoolHmB-B_ak2
        21.0%
        38%
        1.9
        HC5FNDRX5_l02_n02_AtbZIP41_KyoAmpPoolHmB-B_ak1
        27.2%
        38%
        1.9
        HC5FNDRX5_l02_n02_AtbZIP41_KyoAmpPoolHmB-B_ak2
        24.1%
        38%
        2.0
        HC5FNDRX5_l02_n02_AtbZIP41_LerAmpPoolHmB-B_ak1
        27.8%
        39%
        2.1
        HC5FNDRX5_l02_n02_AtbZIP41_LerAmpPoolHmB-B_ak2
        25.1%
        39%
        2.2
        HC5FNDRX5_l02_n02_AtbZIP41_ShaAmpPoolHmB-B_ak1
        26.1%
        38%
        2.1
        HC5FNDRX5_l02_n02_AtbZIP41_ShaAmpPoolHmB-B_ak2
        23.1%
        38%
        2.2
        HC5FNDRX5_l02_n02_AtbZIP44_An1AmpPoolHmB-B_ak1
        26.6%
        41%
        3.1
        HC5FNDRX5_l02_n02_AtbZIP44_An1AmpPoolHmB-B_ak2
        25.7%
        40%
        2.6
        HC5FNDRX5_l02_n02_AtbZIP44_C24AmpPoolHmB-B_ak1
        27.7%
        40%
        4.1
        HC5FNDRX5_l02_n02_AtbZIP44_C24AmpPoolHmB-B_ak2
        26.7%
        40%
        3.5
        HC5FNDRX5_l02_n02_AtbZIP44_ColAmpPoolHmB-B_ak1
        26.5%
        40%
        4.2
        HC5FNDRX5_l02_n02_AtbZIP44_ColAmpPoolHmB-B_ak2
        25.6%
        40%
        3.6
        HC5FNDRX5_l02_n02_AtbZIP44_CviAmpPoolHmB-B_ak1
        27.7%
        40%
        3.8
        HC5FNDRX5_l02_n02_AtbZIP44_CviAmpPoolHmB-B_ak2
        27.1%
        40%
        3.3
        HC5FNDRX5_l02_n02_AtbZIP44_EriAmpPoolHmB-B_ak1
        21.7%
        40%
        3.3
        HC5FNDRX5_l02_n02_AtbZIP44_EriAmpPoolHmB-B_ak2
        20.7%
        39%
        2.7
        HC5FNDRX5_l02_n02_AtbZIP44_KyoAmpPoolHmB-B_ak1
        26.1%
        40%
        3.0
        HC5FNDRX5_l02_n02_AtbZIP44_KyoAmpPoolHmB-B_ak2
        25.2%
        40%
        2.5
        HC5FNDRX5_l02_n02_AtbZIP44_LerAmpPoolHmB-B_ak1
        26.6%
        40%
        3.3
        HC5FNDRX5_l02_n02_AtbZIP44_LerAmpPoolHmB-B_ak2
        25.5%
        40%
        2.7
        HC5FNDRX5_l02_n02_AtbZIP44_ShaAmpPoolHmB-B_ak1
        25.1%
        40%
        4.0
        HC5FNDRX5_l02_n02_AtbZIP44_ShaAmpPoolHmB-B_ak2
        24.3%
        40%
        3.4
        HC5FNDRX5_l02_n02_AtbZIP50_An1AmpPoolHmB-B_ak1
        24.4%
        40%
        2.2
        HC5FNDRX5_l02_n02_AtbZIP50_An1AmpPoolHmB-B_ak2
        23.2%
        40%
        2.0
        HC5FNDRX5_l02_n02_AtbZIP50_C24AmpPoolHmB-B_ak1
        23.9%
        38%
        2.4
        HC5FNDRX5_l02_n02_AtbZIP50_C24AmpPoolHmB-B_ak2
        23.4%
        39%
        2.5
        HC5FNDRX5_l02_n02_AtbZIP50_ColAmpPoolHmB-B_ak1
        24.0%
        39%
        2.5
        HC5FNDRX5_l02_n02_AtbZIP50_ColAmpPoolHmB-B_ak2
        22.8%
        39%
        2.4
        HC5FNDRX5_l02_n02_AtbZIP50_CviAmpPoolHmB-B_ak1
        24.3%
        39%
        2.2
        HC5FNDRX5_l02_n02_AtbZIP50_CviAmpPoolHmB-B_ak2
        23.1%
        39%
        2.1
        HC5FNDRX5_l02_n02_AtbZIP50_EriAmpPoolHmB-B_ak1
        20.2%
        39%
        2.0
        HC5FNDRX5_l02_n02_AtbZIP50_EriAmpPoolHmB-B_ak2
        18.5%
        39%
        1.9
        HC5FNDRX5_l02_n02_AtbZIP50_KyoAmpPoolHmB-B_ak1
        23.5%
        39%
        2.1
        HC5FNDRX5_l02_n02_AtbZIP50_KyoAmpPoolHmB-B_ak2
        21.8%
        39%
        1.9
        HC5FNDRX5_l02_n02_AtbZIP50_LerAmpPoolHmB-B_ak1
        24.7%
        39%
        2.3
        HC5FNDRX5_l02_n02_AtbZIP50_LerAmpPoolHmB-B_ak2
        23.6%
        40%
        2.1
        HC5FNDRX5_l02_n02_AtbZIP50_ShaAmpPoolHmB-B_ak1
        21.7%
        39%
        2.4
        HC5FNDRX5_l02_n02_AtbZIP50_ShaAmpPoolHmB-B_ak2
        20.6%
        39%
        2.3
        HC5FNDRX5_l02_n02_AtbZIP66_An1AmpPoolHmB-B_ak1
        20.8%
        38%
        2.5
        HC5FNDRX5_l02_n02_AtbZIP66_An1AmpPoolHmB-B_ak2
        22.1%
        38%
        3.0
        HC5FNDRX5_l02_n02_AtbZIP66_C24AmpPoolHmB-B_ak1
        21.1%
        37%
        2.8
        HC5FNDRX5_l02_n02_AtbZIP66_C24AmpPoolHmB-B_ak2
        22.5%
        37%
        3.3
        HC5FNDRX5_l02_n02_AtbZIP66_ColAmpPoolHmB-B_ak1
        21.3%
        37%
        3.2
        HC5FNDRX5_l02_n02_AtbZIP66_ColAmpPoolHmB-B_ak2
        22.8%
        37%
        3.8
        HC5FNDRX5_l02_n02_AtbZIP66_CviAmpPoolHmB-B_ak1
        21.4%
        37%
        2.5
        HC5FNDRX5_l02_n02_AtbZIP66_CviAmpPoolHmB-B_ak2
        22.7%
        37%
        3.0
        HC5FNDRX5_l02_n02_AtbZIP66_EriAmpPoolHmB-B_ak1
        15.8%
        37%
        2.1
        HC5FNDRX5_l02_n02_AtbZIP66_EriAmpPoolHmB-B_ak2
        17.0%
        37%
        2.6
        HC5FNDRX5_l02_n02_AtbZIP66_KyoAmpPoolHmB-B_ak1
        20.1%
        37%
        2.4
        HC5FNDRX5_l02_n02_AtbZIP66_KyoAmpPoolHmB-B_ak2
        21.3%
        37%
        2.9
        HC5FNDRX5_l02_n02_AtbZIP66_LerAmpPoolHmB-B_ak1
        21.2%
        38%
        2.5
        HC5FNDRX5_l02_n02_AtbZIP66_LerAmpPoolHmB-B_ak2
        22.6%
        38%
        3.1
        HC5FNDRX5_l02_n02_AtbZIP66_ShaAmpPoolHmB-B_ak1
        17.9%
        37%
        2.6
        HC5FNDRX5_l02_n02_AtbZIP66_ShaAmpPoolHmB-B_ak2
        19.0%
        37%
        3.2
        HC5FNDRX5_l02_n02_pIXHALO_An1AmpPoolHm-B_ak2
        29.8%
        37%
        0.9
        HC5FNDRX5_l02_n02_pIXHALO_C24AmpPoolHm-B_ak2
        28.7%
        36%
        0.7
        HC5FNDRX5_l02_n02_pIXHALO_ColAmpPoolHm-B_ak2
        29.2%
        37%
        1.1
        HC5FNDRX5_l02_n02_pIXHALO_CviAmpPoolHm-B_ak2
        29.4%
        37%
        0.9
        HC5FNDRX5_l02_n02_pIXHALO_EriAmpPoolHm-B_ak2
        27.6%
        36%
        0.7
        HC5FNDRX5_l02_n02_pIXHALO_KyoAmpPoolHm-B_ak2
        28.9%
        37%
        0.8
        HC5FNDRX5_l02_n02_pIXHALO_LerAmpPoolHm-B_ak2
        29.5%
        37%
        0.9
        HC5FNDRX5_l02_n02_pIXHALO_ShaAmpPoolHm-B_ak2
        28.5%
        36%
        0.8
        HC5FNDRX5_l02_n02_undetermined
        61.6%
        42%
        36.6

        Demultiplexing Report


        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool.

        Showing 129/129 rows and 2/2 columns.
        LibraryTotal Read CountPortion (%)
        undetermined_library
        36600907
        10.7
        AtbZIP41_ColAmpPoolHmB-B_ak1
        2559622
        0.8
        AtbZIP41_C24AmpPoolHmB-B_ak1
        1884259
        0.6
        AtbZIP41_EriAmpPoolHmB-B_ak1
        1869491
        0.5
        AtbZIP41_CviAmpPoolHmB-B_ak1
        2132216
        0.6
        AtbZIP41_ShaAmpPoolHmB-B_ak1
        2087449
        0.6
        AtbZIP41_KyoAmpPoolHmB-B_ak1
        1905709
        0.6
        AtbZIP41_LerAmpPoolHmB-B_ak1
        2099626
        0.6
        AtbZIP41_An1AmpPoolHmB-B_ak1
        2183859
        0.6
        AtbZIP41_ColAmpPoolHmB-B_ak2
        2708050
        0.8
        AtbZIP41_C24AmpPoolHmB-B_ak2
        2046218
        0.6
        AtbZIP41_EriAmpPoolHmB-B_ak2
        1910565
        0.6
        AtbZIP41_CviAmpPoolHmB-B_ak2
        2255650
        0.7
        AtbZIP41_ShaAmpPoolHmB-B_ak2
        2231295
        0.7
        AtbZIP41_KyoAmpPoolHmB-B_ak2
        1982552
        0.6
        AtbZIP41_LerAmpPoolHmB-B_ak2
        2199900
        0.6
        AtbZIP41_An1AmpPoolHmB-B_ak2
        2288941
        0.7
        AtbZIP11_ColAmpPoolHmB-B_ak1
        3213212
        0.9
        AtbZIP11_C24AmpPoolHmB-B_ak1
        3346131
        1.0
        AtbZIP11_EriAmpPoolHmB-B_ak1
        2373288
        0.7
        AtbZIP11_CviAmpPoolHmB-B_ak1
        2693506
        0.8
        AtbZIP11_ShaAmpPoolHmB-B_ak1
        2973112
        0.9
        AtbZIP11_KyoAmpPoolHmB-B_ak1
        2442198
        0.7
        AtbZIP11_LerAmpPoolHmB-B_ak1
        2712331
        0.8
        AtbZIP11_An1AmpPoolHmB-B_ak1
        2565110
        0.8
        AtbZIP11_ColAmpPoolHmB-B_ak2
        3255820
        1.0
        AtbZIP11_C24AmpPoolHmB-B_ak2
        3299859
        1.0
        AtbZIP11_EriAmpPoolHmB-B_ak2
        2383866
        0.7
        AtbZIP11_CviAmpPoolHmB-B_ak2
        2759656
        0.8
        AtbZIP11_ShaAmpPoolHmB-B_ak2
        3028310
        0.9
        AtbZIP11_KyoAmpPoolHmB-B_ak2
        2415269
        0.7
        AtbZIP11_LerAmpPoolHmB-B_ak2
        2683316
        0.8
        AtbZIP11_An1AmpPoolHmB-B_ak2
        2584018
        0.8
        AtbZIP3_ColAmpPoolHmB-B_ak1
        2677328
        0.8
        AtbZIP3_C24AmpPoolHmB-B_ak1
        2493019
        0.7
        AtbZIP3_EriAmpPoolHmB-B_ak1
        2009854
        0.6
        AtbZIP3_CviAmpPoolHmB-B_ak1
        2269912
        0.7
        AtbZIP3_ShaAmpPoolHmB-B_ak1
        2406531
        0.7
        AtbZIP3_KyoAmpPoolHmB-B_ak1
        2101677
        0.6
        AtbZIP3_LerAmpPoolHmB-B_ak1
        2255969
        0.7
        AtbZIP3_An1AmpPoolHmB-B_ak1
        2224890
        0.7
        AtbZIP3_ColAmpPoolHmB-B_ak2
        3126853
        0.9
        AtbZIP3_C24AmpPoolHmB-B_ak2
        2920116
        0.9
        AtbZIP3_EriAmpPoolHmB-B_ak2
        2256595
        0.7
        AtbZIP3_CviAmpPoolHmB-B_ak2
        2610951
        0.8
        AtbZIP3_ShaAmpPoolHmB-B_ak2
        2767087
        0.8
        AtbZIP3_KyoAmpPoolHmB-B_ak2
        2385240
        0.7
        AtbZIP3_LerAmpPoolHmB-B_ak2
        2560278
        0.8
        AtbZIP3_An1AmpPoolHmB-B_ak2
        2514278
        0.7
        AtbZIP44_ColAmpPoolHmB-B_ak1
        4152480
        1.2
        AtbZIP44_C24AmpPoolHmB-B_ak1
        4140654
        1.2
        AtbZIP44_EriAmpPoolHmB-B_ak1
        3256719
        1.0
        AtbZIP44_CviAmpPoolHmB-B_ak1
        3842429
        1.1
        AtbZIP44_ShaAmpPoolHmB-B_ak1
        4030010
        1.2
        AtbZIP44_KyoAmpPoolHmB-B_ak1
        3008333
        0.9
        AtbZIP44_LerAmpPoolHmB-B_ak1
        3322238
        1.0
        AtbZIP44_An1AmpPoolHmB-B_ak1
        3137745
        0.9
        AtbZIP44_ColAmpPoolHmB-B_ak2
        3588782
        1.1
        AtbZIP44_C24AmpPoolHmB-B_ak2
        3485363
        1.0
        AtbZIP44_EriAmpPoolHmB-B_ak2
        2736522
        0.8
        AtbZIP44_CviAmpPoolHmB-B_ak2
        3316281
        1.0
        AtbZIP44_ShaAmpPoolHmB-B_ak2
        3407697
        1.0
        AtbZIP44_KyoAmpPoolHmB-B_ak2
        2530437
        0.7
        AtbZIP44_LerAmpPoolHmB-B_ak2
        2740560
        0.8
        AtbZIP44_An1AmpPoolHmB-B_ak2
        2647425
        0.8
        AtbZIP1_ColAmpPoolHmB-B_ak1
        2378371
        0.7
        AtbZIP1_C24AmpPoolHmB-B_ak1
        2004180
        0.6
        AtbZIP1_EriAmpPoolHmB-B_ak1
        1739580
        0.5
        AtbZIP1_CviAmpPoolHmB-B_ak1
        1955719
        0.6
        AtbZIP1_ShaAmpPoolHmB-B_ak1
        1979624
        0.6
        AtbZIP1_KyoAmpPoolHmB-B_ak1
        1822719
        0.5
        AtbZIP1_LerAmpPoolHmB-B_ak1
        1965857
        0.6
        AtbZIP1_An1AmpPoolHmB-B_ak1
        1920136
        0.6
        AtbZIP1_ColAmpPoolHmB-B_ak2
        2632435
        0.8
        AtbZIP1_C24AmpPoolHmB-B_ak2
        2350529
        0.7
        AtbZIP1_EriAmpPoolHmB-B_ak2
        1809337
        0.5
        AtbZIP1_CviAmpPoolHmB-B_ak2
        2130217
        0.6
        AtbZIP1_ShaAmpPoolHmB-B_ak2
        2179815
        0.6
        AtbZIP1_KyoAmpPoolHmB-B_ak2
        1922765
        0.6
        AtbZIP1_LerAmpPoolHmB-B_ak2
        2116527
        0.6
        AtbZIP1_An1AmpPoolHmB-B_ak2
        2050352
        0.6
        AtbZIP66_ColAmpPoolHmB-B_ak1
        3167670
        0.9
        AtbZIP66_C24AmpPoolHmB-B_ak1
        2773638
        0.8
        AtbZIP66_EriAmpPoolHmB-B_ak1
        2130979
        0.6
        AtbZIP66_CviAmpPoolHmB-B_ak1
        2464763
        0.7
        AtbZIP66_ShaAmpPoolHmB-B_ak1
        2613015
        0.8
        AtbZIP66_KyoAmpPoolHmB-B_ak1
        2375990
        0.7
        AtbZIP66_LerAmpPoolHmB-B_ak1
        2545211
        0.7
        AtbZIP66_An1AmpPoolHmB-B_ak1
        2451340
        0.7
        AtbZIP66_ColAmpPoolHmB-B_ak2
        3831348
        1.1
        AtbZIP66_C24AmpPoolHmB-B_ak2
        3340504
        1.0
        AtbZIP66_EriAmpPoolHmB-B_ak2
        2598782
        0.8
        AtbZIP66_CviAmpPoolHmB-B_ak2
        3013538
        0.9
        AtbZIP66_ShaAmpPoolHmB-B_ak2
        3174253
        0.9
        AtbZIP66_KyoAmpPoolHmB-B_ak2
        2856572
        0.8
        AtbZIP66_LerAmpPoolHmB-B_ak2
        3081568
        0.9
        AtbZIP66_An1AmpPoolHmB-B_ak2
        2988213
        0.9
        AtbZIP50_ColAmpPoolHmB-B_ak1
        2530092
        0.7
        AtbZIP50_C24AmpPoolHmB-B_ak1
        2449760
        0.7
        AtbZIP50_EriAmpPoolHmB-B_ak1
        2025201
        0.6
        AtbZIP50_CviAmpPoolHmB-B_ak1
        2183619
        0.6
        AtbZIP50_ShaAmpPoolHmB-B_ak1
        2352869
        0.7
        AtbZIP50_KyoAmpPoolHmB-B_ak1
        2093856
        0.6
        AtbZIP50_LerAmpPoolHmB-B_ak1
        2306763
        0.7
        AtbZIP50_An1AmpPoolHmB-B_ak1
        2183505
        0.6
        AtbZIP50_ColAmpPoolHmB-B_ak2
        2426470
        0.7
        AtbZIP50_C24AmpPoolHmB-B_ak2
        2491373
        0.7
        AtbZIP50_EriAmpPoolHmB-B_ak2
        1864087
        0.5
        AtbZIP50_CviAmpPoolHmB-B_ak2
        2099487
        0.6
        AtbZIP50_ShaAmpPoolHmB-B_ak2
        2281830
        0.7
        AtbZIP50_KyoAmpPoolHmB-B_ak2
        1926240
        0.6
        AtbZIP50_LerAmpPoolHmB-B_ak2
        2126851
        0.6
        AtbZIP50_An1AmpPoolHmB-B_ak2
        2037961
        0.6
        AtbZIP26_ColAmpPoolHmB-B_ak2
        1944867
        0.6
        AtbZIP26_C24AmpPoolHmB-B_ak2
        1337514
        0.4
        AtbZIP26_EriAmpPoolHmB-B_ak2
        1332505
        0.4
        AtbZIP26_CviAmpPoolHmB-B_ak2
        1583822
        0.5
        AtbZIP26_ShaAmpPoolHmB-B_ak2
        1493540
        0.4
        AtbZIP26_KyoAmpPoolHmB-B_ak2
        1467407
        0.4
        AtbZIP26_LerAmpPoolHmB-B_ak2
        1595479
        0.5
        AtbZIP26_An1AmpPoolHmB-B_ak2
        1658739
        0.5
        pIXHALO_ColAmpPoolHm-B_ak2
        1077046
        0.3
        pIXHALO_C24AmpPoolHm-B_ak2
        694000
        0.2
        pIXHALO_EriAmpPoolHm-B_ak2
        722374
        0.2
        pIXHALO_CviAmpPoolHm-B_ak2
        858396
        0.3
        pIXHALO_ShaAmpPoolHm-B_ak2
        773432
        0.2
        pIXHALO_KyoAmpPoolHm-B_ak2
        752983
        0.2
        pIXHALO_LerAmpPoolHm-B_ak2
        851635
        0.2
        pIXHALO_An1AmpPoolHm-B_ak2
        897434
        0.3

        Barcodes of Undetermined Reads


        We have determined the barcodes of your undetermined reads. Here are the top 20 barcodes. The full list is available here. If your libraries are dual indexed, the two indices are concatenated.

        Showing 20/20 rows and 2/2 columns.
        Barcode Sequence(s)CountFrequency (%)
        GGGGGGGGAGATCTCG
        20372103.0
        55.7
        GGGGGGGGCTTCGCCT
        259337.0
        0.7
        GGGGGGGGGCCTCTAT
        251202.0
        0.7
        GGGGGGGGTGGAAGCA
        233463.0
        0.6
        GGGGGGGGAGTTCTCG
        222921.0
        0.6
        GGGGGGGGTCTTACGG
        214276.0
        0.6
        GGGGGGGGAACAGGTG
        209285.0
        0.6
        GGGGGGGGACGTCCTG
        195395.0
        0.5
        GGGGGGGGAGTCGAAG
        187854.0
        0.5
        GGGGGGGGAAGGCGTA
        176935.0
        0.5
        GGGGGGGGGTCAGTAC
        173270.0
        0.5
        GGGGGGGGAGGATAGG
        164150.0
        0.5
        GGGGGGGGCGTGTGAT
        151995.0
        0.4
        GGGGGGGGTAAGATTA
        140515.0
        0.4
        GGGGGGGGCTCGTTCT
        139851.0
        0.4
        GGGGGGGGAGGCTATA
        130426.0
        0.4
        GGGGGGGGAACCTCTC
        83276.0
        0.2
        CGGCTAATGGGGGGGG
        82423.0
        0.2
        ATCGATCGGGGGGGGG
        76580.0
        0.2
        TACGCTACGGGGGGGG
        74145.0
        0.2

        Run Statistics

        Showing 1/1 rows and 4/4 columns.
        LaneTotal # of Single-End ReadsTotal # PF Reads% Undetermined% PhiX Aligned
        2.0
        638337024
        341386248
        10.7
        6.0

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Counts

        Sequence counts for each sample. Duplicate read counts are an estimate only.

        This plot show the total number of reads, broken down into unique and duplicate if possible (only more recent versions of FastQC give duplicate info).

        You can read more about duplicate calculation in the FastQC documentation. A small part has been copied here for convenience:

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Sequence Quality Histograms

        The mean quality value across each base position in the read.

        To enable multiple samples to be plotted on the same graph, only the mean quality scores are plotted (unlike the box plots seen in FastQC reports).

        Taken from the FastQC help:

        The y-axis on the graph shows the quality scores. The higher the score, the better the base call. The background of the graph divides the y axis into very good quality calls (green), calls of reasonable quality (orange), and calls of poor quality (red). The quality of calls on most platforms will degrade as the run progresses, so it is common to see base calls falling into the orange area towards the end of a read.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality.

        From the FastQC help:

        The per sequence quality score report allows you to see if a subset of your sequences have universally low quality values. It is often the case that a subset of sequences will have universally poor quality, however these should represent only a small percentage of the total sequences.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called.

        To enable multiple samples to be shown in a single plot, the base composition data is shown as a heatmap. The colours represent the balance between the four bases: an even distribution should give an even muddy brown colour. Hover over the plot to see the percentage of the four bases under the cursor.

        To see the data as a line plot, as in the original FastQC graph, click on a sample track.

        From the FastQC help:

        Per Base Sequence Content plots out the proportion of each base position in a file for which each of the four normal DNA bases has been called.

        In a random library you would expect that there would be little to no difference between the different bases of a sequence run, so the lines in this plot should run parallel with each other. The relative amount of each base should reflect the overall amount of these bases in your genome, but in any case they should not be hugely imbalanced from each other.

        It's worth noting that some types of library will always produce biased sequence composition, normally at the start of the read. Libraries produced by priming using random hexamers (including nearly all RNA-Seq libraries) and those which were fragmented using transposases inherit an intrinsic bias in the positions at which reads start. This bias does not concern an absolute sequence, but instead provides enrichement of a number of different K-mers at the 5' end of the reads. Whilst this is a true technical bias, it isn't something which can be corrected by trimming and in most cases doesn't seem to adversely affect the downstream analysis.

        Click a sample row to see a line plot for that dataset.
        Rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content.

        From the FastQC help:

        This module measures the GC content across the whole length of each sequence in a file and compares it to a modelled normal distribution of GC content.

        In a normal random library you would expect to see a roughly normal distribution of GC content where the central peak corresponds to the overall GC content of the underlying genome. Since we don't know the the GC content of the genome the modal GC content is calculated from the observed data and used to build a reference distribution.

        An unusually shaped distribution could indicate a contaminated library or some other kinds of biased subset. A normal distribution which is shifted indicates some systematic bias which is independent of base position. If there is a systematic bias which creates a shifted normal distribution then this won't be flagged as an error by the module since it doesn't know what your genome's GC content should be.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Base N Content

        The percentage of base calls at each position for which an N was called.

        From the FastQC help:

        If a sequencer is unable to make a base call with sufficient confidence then it will normally substitute an N rather than a conventional base call. This graph shows the percentage of base calls at each position for which an N was called.

        It's not unusual to see a very low proportion of Ns appearing in a sequence, especially nearer the end of a sequence. However, if this proportion rises above a few percent it suggests that the analysis pipeline was unable to interpret the data well enough to make valid base calls.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Sequence Length Distribution

        All samples have sequences of a single length (101bp).

        Sequence Duplication Levels

        The relative level of duplication found for every sequence.

        From the FastQC Help:

        In a diverse library most sequences will occur only once in the final set. A low level of duplication may indicate a very high level of coverage of the target sequence, but a high level of duplication is more likely to indicate some kind of enrichment bias (eg PCR over amplification). This graph shows the degree of duplication for every sequence in a library: the relative number of sequences with different degrees of duplication.

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        In a properly diverse library most sequences should fall into the far left of the plot in both the red and blue lines. A general level of enrichment, indicating broad oversequencing in the library will tend to flatten the lines, lowering the low end and generally raising other categories. More specific enrichments of subsets, or the presence of low complexity contaminants will tend to produce spikes towards the right of the plot.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Overrepresented sequences

        The total amount of overrepresented sequences found in each library.

        FastQC calculates and lists overrepresented sequences in FastQ files. It would not be possible to show this for all samples in a MultiQC report, so instead this plot shows the number of sequences categorized as over represented.

        Sometimes, a single sequence may account for a large number of reads in a dataset. To show this, the bars are split into two: the first shows the overrepresented reads that come from the single most common sequence. The second shows the total count from all remaining overrepresented sequences.

        From the FastQC Help:

        A normal high-throughput library will contain a diverse set of sequences, with no individual sequence making up a tiny fraction of the whole. Finding that a single sequence is very overrepresented in the set either means that it is highly biologically significant, or indicates that the library is contaminated, or not as diverse as you expected.

        FastQC lists all of the sequences which make up more than 0.1% of the total. To conserve memory only sequences which appear in the first 100,000 sequences are tracked to the end of the file. It is therefore possible that a sequence which is overrepresented but doesn't appear at the start of the file for some reason could be missed by this module.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position.

        Note that only samples with ≥ 0.1% adapter contamination are shown.

        There may be several lines per sample, as one is shown for each adapter detected in the file.

        From the FastQC Help:

        The plot shows a cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. Once a sequence has been seen in a read it is counted as being present right through to the end of the read so the percentages you see will only increase as the read length goes on.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Status Checks

        Status for each FastQC section showing whether results seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

        FastQC assigns a status for each section of the report. These give a quick evaluation of whether the results of the analysis seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

        It is important to stress that although the analysis results appear to give a pass/fail result, these evaluations must be taken in the context of what you expect from your library. A 'normal' sample as far as FastQC is concerned is random and diverse. Some experiments may be expected to produce libraries which are biased in particular ways. You should treat the summary evaluations therefore as pointers to where you should concentrate your attention and understand why your library may not look random and diverse.

        Specific guidance on how to interpret the output of each module can be found in the relevant report section, or in the FastQC help.

        In this heatmap, we summarise all of these into a single heatmap for a quick overview. Note that not all FastQC sections have plots in MultiQC reports, but all status checks are shown in this heatmap.

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