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        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.9

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        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2024-06-14, 15:33 based on data in: /vast/gencore/GENEFLOW/work/25/27c426500c0d442442e6c3981883ab/1


        General Statistics

        Showing 260/260 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        HC5FNDRX5_l01_n01_AtbZIP16_An1AmpPoolHmB-B_ak1
        27.2%
        38%
        2.2
        HC5FNDRX5_l01_n01_AtbZIP16_An1AmpPoolHmB-B_ak2
        25.1%
        39%
        2.6
        HC5FNDRX5_l01_n01_AtbZIP16_C24AmpPoolHmB-B_ak1
        26.0%
        37%
        2.1
        HC5FNDRX5_l01_n01_AtbZIP16_C24AmpPoolHmB-B_ak2
        24.9%
        38%
        2.7
        HC5FNDRX5_l01_n01_AtbZIP16_ColAmpPoolHmB-B_ak1
        27.3%
        38%
        2.6
        HC5FNDRX5_l01_n01_AtbZIP16_ColAmpPoolHmB-B_ak2
        25.4%
        38%
        3.2
        HC5FNDRX5_l01_n01_AtbZIP16_CviAmpPoolHmB-B_ak1
        27.1%
        38%
        2.3
        HC5FNDRX5_l01_n01_AtbZIP16_CviAmpPoolHmB-B_ak2
        25.4%
        38%
        2.7
        HC5FNDRX5_l01_n01_AtbZIP16_EriAmpPoolHmB-B_ak1
        22.8%
        37%
        2.0
        HC5FNDRX5_l01_n01_AtbZIP16_EriAmpPoolHmB-B_ak2
        20.0%
        38%
        2.4
        HC5FNDRX5_l01_n01_AtbZIP16_KyoAmpPoolHmB-B_ak1
        26.7%
        38%
        2.0
        HC5FNDRX5_l01_n01_AtbZIP16_KyoAmpPoolHmB-B_ak2
        24.5%
        38%
        2.4
        HC5FNDRX5_l01_n01_AtbZIP16_LerAmpPoolHmB-B_ak1
        27.6%
        38%
        2.2
        HC5FNDRX5_l01_n01_AtbZIP16_LerAmpPoolHmB-B_ak2
        25.5%
        38%
        2.6
        HC5FNDRX5_l01_n01_AtbZIP16_ShaAmpPoolHmB-B_ak1
        24.3%
        37%
        2.2
        HC5FNDRX5_l01_n01_AtbZIP16_ShaAmpPoolHmB-B_ak2
        22.3%
        38%
        2.8
        HC5FNDRX5_l01_n01_AtbZIP17_An1AmpPoolHmA-B_ak1
        29.6%
        39%
        2.3
        HC5FNDRX5_l01_n01_AtbZIP17_An1AmpPoolHmA-B_ak2
        25.9%
        39%
        2.2
        HC5FNDRX5_l01_n01_AtbZIP17_C24AmpPoolHmA-B_ak1
        29.8%
        38%
        2.7
        HC5FNDRX5_l01_n01_AtbZIP17_C24AmpPoolHmA-B_ak2
        26.9%
        39%
        2.5
        HC5FNDRX5_l01_n01_AtbZIP17_ColAmpPoolHmA-B_ak1
        29.1%
        39%
        2.5
        HC5FNDRX5_l01_n01_AtbZIP17_ColAmpPoolHmA-B_ak2
        25.8%
        39%
        2.5
        HC5FNDRX5_l01_n01_AtbZIP17_CviAmpPoolHmA-B_ak1
        29.5%
        39%
        2.6
        HC5FNDRX5_l01_n01_AtbZIP17_CviAmpPoolHmA-B_ak2
        25.8%
        39%
        2.4
        HC5FNDRX5_l01_n01_AtbZIP17_EriAmpPoolHmA-B_ak1
        25.3%
        38%
        2.1
        HC5FNDRX5_l01_n01_AtbZIP17_EriAmpPoolHmA-B_ak2
        21.2%
        38%
        1.9
        HC5FNDRX5_l01_n01_AtbZIP17_KyoAmpPoolHmA-B_ak1
        30.5%
        39%
        2.6
        HC5FNDRX5_l01_n01_AtbZIP17_KyoAmpPoolHmA-B_ak2
        27.4%
        40%
        2.5
        HC5FNDRX5_l01_n01_AtbZIP17_LerAmpPoolHmA-B_ak1
        31.4%
        39%
        3.1
        HC5FNDRX5_l01_n01_AtbZIP17_LerAmpPoolHmA-B_ak2
        27.8%
        40%
        2.8
        HC5FNDRX5_l01_n01_AtbZIP17_ShaAmpPoolHmA-B_ak1
        26.7%
        39%
        2.4
        HC5FNDRX5_l01_n01_AtbZIP17_ShaAmpPoolHmA-B_ak2
        23.7%
        39%
        2.3
        HC5FNDRX5_l01_n01_AtbZIP18_An1AmpPoolHmB-B_ak1
        35.3%
        38%
        2.5
        HC5FNDRX5_l01_n01_AtbZIP18_An1AmpPoolHmB-B_ak2
        34.1%
        37%
        2.8
        HC5FNDRX5_l01_n01_AtbZIP18_C24AmpPoolHmB-B_ak1
        33.0%
        37%
        1.9
        HC5FNDRX5_l01_n01_AtbZIP18_C24AmpPoolHmB-B_ak2
        31.6%
        37%
        2.2
        HC5FNDRX5_l01_n01_AtbZIP18_ColAmpPoolHmB-B_ak1
        34.8%
        38%
        2.4
        HC5FNDRX5_l01_n01_AtbZIP18_ColAmpPoolHmB-B_ak2
        33.4%
        37%
        2.7
        HC5FNDRX5_l01_n01_AtbZIP18_CviAmpPoolHmB-B_ak1
        34.1%
        37%
        2.1
        HC5FNDRX5_l01_n01_AtbZIP18_CviAmpPoolHmB-B_ak2
        32.5%
        37%
        2.3
        HC5FNDRX5_l01_n01_AtbZIP18_EriAmpPoolHmB-B_ak1
        31.0%
        37%
        1.9
        HC5FNDRX5_l01_n01_AtbZIP18_EriAmpPoolHmB-B_ak2
        29.4%
        36%
        2.2
        HC5FNDRX5_l01_n01_AtbZIP18_KyoAmpPoolHmB-B_ak1
        34.4%
        38%
        1.9
        HC5FNDRX5_l01_n01_AtbZIP18_KyoAmpPoolHmB-B_ak2
        32.9%
        37%
        2.2
        HC5FNDRX5_l01_n01_AtbZIP18_LerAmpPoolHmB-B_ak1
        35.4%
        38%
        2.1
        HC5FNDRX5_l01_n01_AtbZIP18_LerAmpPoolHmB-B_ak2
        33.9%
        37%
        2.4
        HC5FNDRX5_l01_n01_AtbZIP18_ShaAmpPoolHmB-B_ak1
        30.9%
        37%
        1.9
        HC5FNDRX5_l01_n01_AtbZIP18_ShaAmpPoolHmB-B_ak2
        29.4%
        37%
        2.2
        HC5FNDRX5_l01_n01_AtbZIP21_An1AmpPoolHmB-B_ak1
        77.9%
        37%
        5.4
        HC5FNDRX5_l01_n01_AtbZIP21_An1AmpPoolHmB-B_ak2
        73.9%
        38%
        4.1
        HC5FNDRX5_l01_n01_AtbZIP21_C24AmpPoolHmB-B_ak1
        78.3%
        36%
        4.9
        HC5FNDRX5_l01_n01_AtbZIP21_C24AmpPoolHmB-B_ak2
        74.6%
        37%
        3.9
        HC5FNDRX5_l01_n01_AtbZIP21_ColAmpPoolHmB-B_ak1
        78.0%
        37%
        6.8
        HC5FNDRX5_l01_n01_AtbZIP21_ColAmpPoolHmB-B_ak2
        74.5%
        37%
        5.4
        HC5FNDRX5_l01_n01_AtbZIP21_CviAmpPoolHmB-B_ak1
        77.8%
        37%
        5.3
        HC5FNDRX5_l01_n01_AtbZIP21_CviAmpPoolHmB-B_ak2
        73.9%
        37%
        4.0
        HC5FNDRX5_l01_n01_AtbZIP21_EriAmpPoolHmB-B_ak1
        77.5%
        36%
        4.3
        HC5FNDRX5_l01_n01_AtbZIP21_EriAmpPoolHmB-B_ak2
        73.0%
        36%
        3.2
        HC5FNDRX5_l01_n01_AtbZIP21_KyoAmpPoolHmB-B_ak1
        78.0%
        37%
        4.7
        HC5FNDRX5_l01_n01_AtbZIP21_KyoAmpPoolHmB-B_ak2
        73.9%
        37%
        3.6
        HC5FNDRX5_l01_n01_AtbZIP21_LerAmpPoolHmB-B_ak1
        78.1%
        37%
        5.5
        HC5FNDRX5_l01_n01_AtbZIP21_LerAmpPoolHmB-B_ak2
        74.2%
        37%
        4.2
        HC5FNDRX5_l01_n01_AtbZIP21_ShaAmpPoolHmB-B_ak1
        78.1%
        36%
        4.9
        HC5FNDRX5_l01_n01_AtbZIP21_ShaAmpPoolHmB-B_ak2
        74.0%
        37%
        3.7
        HC5FNDRX5_l01_n01_AtbZIP22_An1AmpPoolHmB-B_ak1
        26.7%
        38%
        2.6
        HC5FNDRX5_l01_n01_AtbZIP22_An1AmpPoolHmB-B_ak2
        27.2%
        38%
        3.1
        HC5FNDRX5_l01_n01_AtbZIP22_C24AmpPoolHmB-B_ak1
        26.5%
        37%
        2.9
        HC5FNDRX5_l01_n01_AtbZIP22_C24AmpPoolHmB-B_ak2
        27.1%
        37%
        3.7
        HC5FNDRX5_l01_n01_AtbZIP22_ColAmpPoolHmB-B_ak1
        26.3%
        38%
        3.0
        HC5FNDRX5_l01_n01_AtbZIP22_ColAmpPoolHmB-B_ak2
        26.7%
        38%
        3.8
        HC5FNDRX5_l01_n01_AtbZIP22_CviAmpPoolHmB-B_ak1
        26.8%
        38%
        2.6
        HC5FNDRX5_l01_n01_AtbZIP22_CviAmpPoolHmB-B_ak2
        27.6%
        38%
        3.2
        HC5FNDRX5_l01_n01_AtbZIP22_EriAmpPoolHmB-B_ak1
        21.1%
        37%
        2.3
        HC5FNDRX5_l01_n01_AtbZIP22_EriAmpPoolHmB-B_ak2
        21.5%
        37%
        2.8
        HC5FNDRX5_l01_n01_AtbZIP22_KyoAmpPoolHmB-B_ak1
        25.6%
        38%
        2.5
        HC5FNDRX5_l01_n01_AtbZIP22_KyoAmpPoolHmB-B_ak2
        26.1%
        38%
        3.0
        HC5FNDRX5_l01_n01_AtbZIP22_LerAmpPoolHmB-B_ak1
        26.6%
        38%
        2.6
        HC5FNDRX5_l01_n01_AtbZIP22_LerAmpPoolHmB-B_ak2
        27.3%
        38%
        3.2
        HC5FNDRX5_l01_n01_AtbZIP22_ShaAmpPoolHmB-B_ak1
        23.8%
        38%
        2.8
        HC5FNDRX5_l01_n01_AtbZIP22_ShaAmpPoolHmB-B_ak2
        24.8%
        38%
        3.5
        HC5FNDRX5_l01_n01_AtbZIP23_An1AmpPoolHmB-B_ak1
        50.1%
        37%
        2.4
        HC5FNDRX5_l01_n01_AtbZIP23_An1AmpPoolHmB-B_ak2
        39.3%
        37%
        2.2
        HC5FNDRX5_l01_n01_AtbZIP23_C24AmpPoolHmB-B_ak1
        48.0%
        36%
        1.9
        HC5FNDRX5_l01_n01_AtbZIP23_C24AmpPoolHmB-B_ak2
        36.5%
        35%
        1.6
        HC5FNDRX5_l01_n01_AtbZIP23_ColAmpPoolHmB-B_ak1
        49.7%
        36%
        2.9
        HC5FNDRX5_l01_n01_AtbZIP23_ColAmpPoolHmB-B_ak2
        39.1%
        36%
        2.8
        HC5FNDRX5_l01_n01_AtbZIP23_CviAmpPoolHmB-B_ak1
        49.8%
        36%
        2.4
        HC5FNDRX5_l01_n01_AtbZIP23_CviAmpPoolHmB-B_ak2
        38.6%
        36%
        2.1
        HC5FNDRX5_l01_n01_AtbZIP23_EriAmpPoolHmB-B_ak1
        47.5%
        36%
        2.0
        HC5FNDRX5_l01_n01_AtbZIP23_EriAmpPoolHmB-B_ak2
        35.3%
        36%
        1.7
        HC5FNDRX5_l01_n01_AtbZIP23_KyoAmpPoolHmB-B_ak1
        49.6%
        36%
        2.2
        HC5FNDRX5_l01_n01_AtbZIP23_KyoAmpPoolHmB-B_ak2
        38.4%
        36%
        1.9
        HC5FNDRX5_l01_n01_AtbZIP23_LerAmpPoolHmB-B_ak1
        49.7%
        37%
        2.3
        HC5FNDRX5_l01_n01_AtbZIP23_LerAmpPoolHmB-B_ak2
        39.1%
        36%
        2.1
        HC5FNDRX5_l01_n01_AtbZIP23_ShaAmpPoolHmB-B_ak1
        48.1%
        36%
        2.1
        HC5FNDRX5_l01_n01_AtbZIP23_ShaAmpPoolHmB-B_ak2
        36.1%
        36%
        1.9
        HC5FNDRX5_l01_n01_AtbZIP26_An1AmpPoolHmB-B_ak1
        34.8%
        37%
        2.1
        HC5FNDRX5_l01_n01_AtbZIP26_C24AmpPoolHmB-B_ak1
        32.7%
        36%
        1.7
        HC5FNDRX5_l01_n01_AtbZIP26_ColAmpPoolHmB-B_ak1
        34.5%
        37%
        2.5
        HC5FNDRX5_l01_n01_AtbZIP26_CviAmpPoolHmB-B_ak1
        34.5%
        37%
        2.1
        HC5FNDRX5_l01_n01_AtbZIP26_EriAmpPoolHmB-B_ak1
        30.5%
        36%
        1.7
        HC5FNDRX5_l01_n01_AtbZIP26_KyoAmpPoolHmB-B_ak1
        34.0%
        37%
        1.9
        HC5FNDRX5_l01_n01_AtbZIP26_LerAmpPoolHmB-B_ak1
        34.6%
        37%
        2.0
        HC5FNDRX5_l01_n01_AtbZIP26_ShaAmpPoolHmB-B_ak1
        31.8%
        37%
        1.9
        HC5FNDRX5_l01_n01_AtbZIP2_An1AmpPoolHmB-B_ak1
        37.4%
        37%
        2.3
        HC5FNDRX5_l01_n01_AtbZIP2_An1AmpPoolHmB-B_ak2
        30.0%
        37%
        2.7
        HC5FNDRX5_l01_n01_AtbZIP2_C24AmpPoolHmB-B_ak1
        35.8%
        36%
        2.0
        HC5FNDRX5_l01_n01_AtbZIP2_C24AmpPoolHmB-B_ak2
        28.7%
        36%
        2.4
        HC5FNDRX5_l01_n01_AtbZIP2_ColAmpPoolHmB-B_ak1
        37.8%
        37%
        2.8
        HC5FNDRX5_l01_n01_AtbZIP2_ColAmpPoolHmB-B_ak2
        30.6%
        37%
        3.3
        HC5FNDRX5_l01_n01_AtbZIP2_CviAmpPoolHmB-B_ak1
        36.8%
        37%
        2.3
        HC5FNDRX5_l01_n01_AtbZIP2_CviAmpPoolHmB-B_ak2
        30.0%
        37%
        2.7
        HC5FNDRX5_l01_n01_AtbZIP2_EriAmpPoolHmB-B_ak1
        34.1%
        36%
        2.0
        HC5FNDRX5_l01_n01_AtbZIP2_EriAmpPoolHmB-B_ak2
        26.0%
        36%
        2.3
        HC5FNDRX5_l01_n01_AtbZIP2_KyoAmpPoolHmB-B_ak1
        37.1%
        37%
        2.1
        HC5FNDRX5_l01_n01_AtbZIP2_KyoAmpPoolHmB-B_ak2
        29.5%
        37%
        2.4
        HC5FNDRX5_l01_n01_AtbZIP2_LerAmpPoolHmB-B_ak1
        37.7%
        37%
        2.2
        HC5FNDRX5_l01_n01_AtbZIP2_LerAmpPoolHmB-B_ak2
        30.9%
        37%
        2.7
        HC5FNDRX5_l01_n01_AtbZIP2_ShaAmpPoolHmB-B_ak1
        34.1%
        37%
        2.1
        HC5FNDRX5_l01_n01_AtbZIP2_ShaAmpPoolHmB-B_ak2
        26.5%
        37%
        2.6
        HC5FNDRX5_l01_n01_PdaTF1_Pda-B_ak
        22.2%
        38%
        3.9
        HC5FNDRX5_l01_n01_pIXHALO_An1AmpPoolHmB-B_ak1
        49.8%
        36%
        1.0
        HC5FNDRX5_l01_n01_pIXHALO_C24AmpPoolHmB-B_ak1
        48.4%
        35%
        0.9
        HC5FNDRX5_l01_n01_pIXHALO_ColAmpPoolHmB-B_ak1
        49.6%
        36%
        1.3
        HC5FNDRX5_l01_n01_pIXHALO_CviAmpPoolHmB-B_ak1
        49.2%
        36%
        1.0
        HC5FNDRX5_l01_n01_pIXHALO_EriAmpPoolHmB-B_ak1
        48.4%
        35%
        0.9
        HC5FNDRX5_l01_n01_pIXHALO_KyoAmpPoolHmB-B_ak1
        49.5%
        36%
        0.9
        HC5FNDRX5_l01_n01_pIXHALO_LerAmpPoolHmB-B_ak1
        49.0%
        36%
        1.0
        HC5FNDRX5_l01_n01_pIXHALO_ShaAmpPoolHmB-B_ak1
        48.6%
        36%
        0.9
        HC5FNDRX5_l01_n01_undetermined
        71.8%
        41%
        42.7
        HC5FNDRX5_l01_n02_AtbZIP16_An1AmpPoolHmB-B_ak1
        25.7%
        38%
        2.2
        HC5FNDRX5_l01_n02_AtbZIP16_An1AmpPoolHmB-B_ak2
        24.0%
        39%
        2.6
        HC5FNDRX5_l01_n02_AtbZIP16_C24AmpPoolHmB-B_ak1
        24.9%
        37%
        2.1
        HC5FNDRX5_l01_n02_AtbZIP16_C24AmpPoolHmB-B_ak2
        24.4%
        38%
        2.7
        HC5FNDRX5_l01_n02_AtbZIP16_ColAmpPoolHmB-B_ak1
        25.9%
        38%
        2.6
        HC5FNDRX5_l01_n02_AtbZIP16_ColAmpPoolHmB-B_ak2
        24.6%
        38%
        3.2
        HC5FNDRX5_l01_n02_AtbZIP16_CviAmpPoolHmB-B_ak1
        25.7%
        38%
        2.3
        HC5FNDRX5_l01_n02_AtbZIP16_CviAmpPoolHmB-B_ak2
        24.7%
        38%
        2.7
        HC5FNDRX5_l01_n02_AtbZIP16_EriAmpPoolHmB-B_ak1
        21.7%
        37%
        2.0
        HC5FNDRX5_l01_n02_AtbZIP16_EriAmpPoolHmB-B_ak2
        19.3%
        38%
        2.4
        HC5FNDRX5_l01_n02_AtbZIP16_KyoAmpPoolHmB-B_ak1
        25.0%
        38%
        2.0
        HC5FNDRX5_l01_n02_AtbZIP16_KyoAmpPoolHmB-B_ak2
        23.6%
        38%
        2.4
        HC5FNDRX5_l01_n02_AtbZIP16_LerAmpPoolHmB-B_ak1
        25.9%
        38%
        2.2
        HC5FNDRX5_l01_n02_AtbZIP16_LerAmpPoolHmB-B_ak2
        24.6%
        38%
        2.6
        HC5FNDRX5_l01_n02_AtbZIP16_ShaAmpPoolHmB-B_ak1
        22.8%
        38%
        2.2
        HC5FNDRX5_l01_n02_AtbZIP16_ShaAmpPoolHmB-B_ak2
        21.4%
        38%
        2.8
        HC5FNDRX5_l01_n02_AtbZIP17_An1AmpPoolHmA-B_ak1
        27.9%
        39%
        2.3
        HC5FNDRX5_l01_n02_AtbZIP17_An1AmpPoolHmA-B_ak2
        24.8%
        39%
        2.2
        HC5FNDRX5_l01_n02_AtbZIP17_C24AmpPoolHmA-B_ak1
        28.1%
        38%
        2.7
        HC5FNDRX5_l01_n02_AtbZIP17_C24AmpPoolHmA-B_ak2
        26.0%
        39%
        2.5
        HC5FNDRX5_l01_n02_AtbZIP17_ColAmpPoolHmA-B_ak1
        27.4%
        39%
        2.5
        HC5FNDRX5_l01_n02_AtbZIP17_ColAmpPoolHmA-B_ak2
        24.5%
        39%
        2.5
        HC5FNDRX5_l01_n02_AtbZIP17_CviAmpPoolHmA-B_ak1
        27.9%
        39%
        2.6
        HC5FNDRX5_l01_n02_AtbZIP17_CviAmpPoolHmA-B_ak2
        24.7%
        39%
        2.4
        HC5FNDRX5_l01_n02_AtbZIP17_EriAmpPoolHmA-B_ak1
        24.0%
        38%
        2.1
        HC5FNDRX5_l01_n02_AtbZIP17_EriAmpPoolHmA-B_ak2
        20.3%
        38%
        1.9
        HC5FNDRX5_l01_n02_AtbZIP17_KyoAmpPoolHmA-B_ak1
        28.7%
        39%
        2.6
        HC5FNDRX5_l01_n02_AtbZIP17_KyoAmpPoolHmA-B_ak2
        26.1%
        40%
        2.5
        HC5FNDRX5_l01_n02_AtbZIP17_LerAmpPoolHmA-B_ak1
        29.8%
        39%
        3.1
        HC5FNDRX5_l01_n02_AtbZIP17_LerAmpPoolHmA-B_ak2
        26.4%
        39%
        2.8
        HC5FNDRX5_l01_n02_AtbZIP17_ShaAmpPoolHmA-B_ak1
        25.1%
        39%
        2.4
        HC5FNDRX5_l01_n02_AtbZIP17_ShaAmpPoolHmA-B_ak2
        22.5%
        39%
        2.3
        HC5FNDRX5_l01_n02_AtbZIP18_An1AmpPoolHmB-B_ak1
        33.5%
        38%
        2.5
        HC5FNDRX5_l01_n02_AtbZIP18_An1AmpPoolHmB-B_ak2
        32.5%
        37%
        2.8
        HC5FNDRX5_l01_n02_AtbZIP18_C24AmpPoolHmB-B_ak1
        31.8%
        37%
        1.9
        HC5FNDRX5_l01_n02_AtbZIP18_C24AmpPoolHmB-B_ak2
        30.5%
        36%
        2.2
        HC5FNDRX5_l01_n02_AtbZIP18_ColAmpPoolHmB-B_ak1
        33.1%
        38%
        2.4
        HC5FNDRX5_l01_n02_AtbZIP18_ColAmpPoolHmB-B_ak2
        31.9%
        37%
        2.7
        HC5FNDRX5_l01_n02_AtbZIP18_CviAmpPoolHmB-B_ak1
        32.4%
        37%
        2.1
        HC5FNDRX5_l01_n02_AtbZIP18_CviAmpPoolHmB-B_ak2
        31.1%
        37%
        2.3
        HC5FNDRX5_l01_n02_AtbZIP18_EriAmpPoolHmB-B_ak1
        29.5%
        37%
        1.9
        HC5FNDRX5_l01_n02_AtbZIP18_EriAmpPoolHmB-B_ak2
        28.0%
        36%
        2.2
        HC5FNDRX5_l01_n02_AtbZIP18_KyoAmpPoolHmB-B_ak1
        32.7%
        38%
        1.9
        HC5FNDRX5_l01_n02_AtbZIP18_KyoAmpPoolHmB-B_ak2
        31.4%
        37%
        2.2
        HC5FNDRX5_l01_n02_AtbZIP18_LerAmpPoolHmB-B_ak1
        33.7%
        38%
        2.1
        HC5FNDRX5_l01_n02_AtbZIP18_LerAmpPoolHmB-B_ak2
        32.4%
        37%
        2.4
        HC5FNDRX5_l01_n02_AtbZIP18_ShaAmpPoolHmB-B_ak1
        29.5%
        37%
        1.9
        HC5FNDRX5_l01_n02_AtbZIP18_ShaAmpPoolHmB-B_ak2
        27.8%
        37%
        2.2
        HC5FNDRX5_l01_n02_AtbZIP21_An1AmpPoolHmB-B_ak1
        76.0%
        37%
        5.4
        HC5FNDRX5_l01_n02_AtbZIP21_An1AmpPoolHmB-B_ak2
        71.7%
        38%
        4.1
        HC5FNDRX5_l01_n02_AtbZIP21_C24AmpPoolHmB-B_ak1
        77.1%
        36%
        4.9
        HC5FNDRX5_l01_n02_AtbZIP21_C24AmpPoolHmB-B_ak2
        72.7%
        37%
        3.9
        HC5FNDRX5_l01_n02_AtbZIP21_ColAmpPoolHmB-B_ak1
        76.3%
        37%
        6.8
        HC5FNDRX5_l01_n02_AtbZIP21_ColAmpPoolHmB-B_ak2
        72.3%
        37%
        5.4
        HC5FNDRX5_l01_n02_AtbZIP21_CviAmpPoolHmB-B_ak1
        76.1%
        37%
        5.3
        HC5FNDRX5_l01_n02_AtbZIP21_CviAmpPoolHmB-B_ak2
        71.8%
        37%
        4.0
        HC5FNDRX5_l01_n02_AtbZIP21_EriAmpPoolHmB-B_ak1
        75.9%
        36%
        4.3
        HC5FNDRX5_l01_n02_AtbZIP21_EriAmpPoolHmB-B_ak2
        70.9%
        36%
        3.2
        HC5FNDRX5_l01_n02_AtbZIP21_KyoAmpPoolHmB-B_ak1
        76.2%
        37%
        4.7
        HC5FNDRX5_l01_n02_AtbZIP21_KyoAmpPoolHmB-B_ak2
        71.8%
        37%
        3.6
        HC5FNDRX5_l01_n02_AtbZIP21_LerAmpPoolHmB-B_ak1
        76.1%
        37%
        5.5
        HC5FNDRX5_l01_n02_AtbZIP21_LerAmpPoolHmB-B_ak2
        72.0%
        37%
        4.2
        HC5FNDRX5_l01_n02_AtbZIP21_ShaAmpPoolHmB-B_ak1
        76.3%
        36%
        4.9
        HC5FNDRX5_l01_n02_AtbZIP21_ShaAmpPoolHmB-B_ak2
        71.6%
        37%
        3.7
        HC5FNDRX5_l01_n02_AtbZIP22_An1AmpPoolHmB-B_ak1
        25.6%
        38%
        2.6
        HC5FNDRX5_l01_n02_AtbZIP22_An1AmpPoolHmB-B_ak2
        26.5%
        38%
        3.1
        HC5FNDRX5_l01_n02_AtbZIP22_C24AmpPoolHmB-B_ak1
        25.7%
        38%
        2.9
        HC5FNDRX5_l01_n02_AtbZIP22_C24AmpPoolHmB-B_ak2
        26.8%
        37%
        3.7
        HC5FNDRX5_l01_n02_AtbZIP22_ColAmpPoolHmB-B_ak1
        25.3%
        38%
        3.0
        HC5FNDRX5_l01_n02_AtbZIP22_ColAmpPoolHmB-B_ak2
        26.0%
        38%
        3.8
        HC5FNDRX5_l01_n02_AtbZIP22_CviAmpPoolHmB-B_ak1
        25.9%
        38%
        2.6
        HC5FNDRX5_l01_n02_AtbZIP22_CviAmpPoolHmB-B_ak2
        27.3%
        38%
        3.2
        HC5FNDRX5_l01_n02_AtbZIP22_EriAmpPoolHmB-B_ak1
        20.4%
        37%
        2.3
        HC5FNDRX5_l01_n02_AtbZIP22_EriAmpPoolHmB-B_ak2
        21.0%
        37%
        2.8
        HC5FNDRX5_l01_n02_AtbZIP22_KyoAmpPoolHmB-B_ak1
        24.5%
        38%
        2.5
        HC5FNDRX5_l01_n02_AtbZIP22_KyoAmpPoolHmB-B_ak2
        25.3%
        38%
        3.0
        HC5FNDRX5_l01_n02_AtbZIP22_LerAmpPoolHmB-B_ak1
        25.7%
        38%
        2.6
        HC5FNDRX5_l01_n02_AtbZIP22_LerAmpPoolHmB-B_ak2
        26.6%
        38%
        3.2
        HC5FNDRX5_l01_n02_AtbZIP22_ShaAmpPoolHmB-B_ak1
        22.7%
        38%
        2.8
        HC5FNDRX5_l01_n02_AtbZIP22_ShaAmpPoolHmB-B_ak2
        24.0%
        38%
        3.5
        HC5FNDRX5_l01_n02_AtbZIP23_An1AmpPoolHmB-B_ak1
        47.6%
        37%
        2.4
        HC5FNDRX5_l01_n02_AtbZIP23_An1AmpPoolHmB-B_ak2
        37.0%
        37%
        2.2
        HC5FNDRX5_l01_n02_AtbZIP23_C24AmpPoolHmB-B_ak1
        46.1%
        36%
        1.9
        HC5FNDRX5_l01_n02_AtbZIP23_C24AmpPoolHmB-B_ak2
        35.1%
        35%
        1.6
        HC5FNDRX5_l01_n02_AtbZIP23_ColAmpPoolHmB-B_ak1
        47.5%
        37%
        2.9
        HC5FNDRX5_l01_n02_AtbZIP23_ColAmpPoolHmB-B_ak2
        37.3%
        36%
        2.8
        HC5FNDRX5_l01_n02_AtbZIP23_CviAmpPoolHmB-B_ak1
        47.2%
        37%
        2.4
        HC5FNDRX5_l01_n02_AtbZIP23_CviAmpPoolHmB-B_ak2
        36.6%
        36%
        2.1
        HC5FNDRX5_l01_n02_AtbZIP23_EriAmpPoolHmB-B_ak1
        45.2%
        36%
        2.0
        HC5FNDRX5_l01_n02_AtbZIP23_EriAmpPoolHmB-B_ak2
        33.7%
        36%
        1.7
        HC5FNDRX5_l01_n02_AtbZIP23_KyoAmpPoolHmB-B_ak1
        47.0%
        36%
        2.2
        HC5FNDRX5_l01_n02_AtbZIP23_KyoAmpPoolHmB-B_ak2
        36.2%
        36%
        1.9
        HC5FNDRX5_l01_n02_AtbZIP23_LerAmpPoolHmB-B_ak1
        47.3%
        37%
        2.3
        HC5FNDRX5_l01_n02_AtbZIP23_LerAmpPoolHmB-B_ak2
        37.1%
        36%
        2.1
        HC5FNDRX5_l01_n02_AtbZIP23_ShaAmpPoolHmB-B_ak1
        45.6%
        36%
        2.1
        HC5FNDRX5_l01_n02_AtbZIP23_ShaAmpPoolHmB-B_ak2
        34.0%
        36%
        1.9
        HC5FNDRX5_l01_n02_AtbZIP26_An1AmpPoolHmB-B_ak1
        33.0%
        37%
        2.1
        HC5FNDRX5_l01_n02_AtbZIP26_C24AmpPoolHmB-B_ak1
        31.3%
        36%
        1.7
        HC5FNDRX5_l01_n02_AtbZIP26_ColAmpPoolHmB-B_ak1
        32.9%
        37%
        2.5
        HC5FNDRX5_l01_n02_AtbZIP26_CviAmpPoolHmB-B_ak1
        32.8%
        37%
        2.1
        HC5FNDRX5_l01_n02_AtbZIP26_EriAmpPoolHmB-B_ak1
        29.0%
        36%
        1.7
        HC5FNDRX5_l01_n02_AtbZIP26_KyoAmpPoolHmB-B_ak1
        32.1%
        37%
        1.9
        HC5FNDRX5_l01_n02_AtbZIP26_LerAmpPoolHmB-B_ak1
        33.0%
        37%
        2.0
        HC5FNDRX5_l01_n02_AtbZIP26_ShaAmpPoolHmB-B_ak1
        30.2%
        37%
        1.9
        HC5FNDRX5_l01_n02_AtbZIP2_An1AmpPoolHmB-B_ak1
        35.5%
        37%
        2.3
        HC5FNDRX5_l01_n02_AtbZIP2_An1AmpPoolHmB-B_ak2
        28.6%
        37%
        2.7
        HC5FNDRX5_l01_n02_AtbZIP2_C24AmpPoolHmB-B_ak1
        34.3%
        36%
        2.0
        HC5FNDRX5_l01_n02_AtbZIP2_C24AmpPoolHmB-B_ak2
        27.6%
        36%
        2.4
        HC5FNDRX5_l01_n02_AtbZIP2_ColAmpPoolHmB-B_ak1
        36.1%
        37%
        2.8
        HC5FNDRX5_l01_n02_AtbZIP2_ColAmpPoolHmB-B_ak2
        29.3%
        37%
        3.3
        HC5FNDRX5_l01_n02_AtbZIP2_CviAmpPoolHmB-B_ak1
        35.1%
        37%
        2.3
        HC5FNDRX5_l01_n02_AtbZIP2_CviAmpPoolHmB-B_ak2
        28.7%
        37%
        2.7
        HC5FNDRX5_l01_n02_AtbZIP2_EriAmpPoolHmB-B_ak1
        32.3%
        36%
        2.0
        HC5FNDRX5_l01_n02_AtbZIP2_EriAmpPoolHmB-B_ak2
        24.9%
        36%
        2.3
        HC5FNDRX5_l01_n02_AtbZIP2_KyoAmpPoolHmB-B_ak1
        35.0%
        37%
        2.1
        HC5FNDRX5_l01_n02_AtbZIP2_KyoAmpPoolHmB-B_ak2
        28.1%
        37%
        2.4
        HC5FNDRX5_l01_n02_AtbZIP2_LerAmpPoolHmB-B_ak1
        35.7%
        37%
        2.2
        HC5FNDRX5_l01_n02_AtbZIP2_LerAmpPoolHmB-B_ak2
        29.4%
        37%
        2.7
        HC5FNDRX5_l01_n02_AtbZIP2_ShaAmpPoolHmB-B_ak1
        32.4%
        37%
        2.1
        HC5FNDRX5_l01_n02_AtbZIP2_ShaAmpPoolHmB-B_ak2
        25.2%
        37%
        2.6
        HC5FNDRX5_l01_n02_PdaTF1_Pda-B_ak
        21.7%
        38%
        3.9
        HC5FNDRX5_l01_n02_pIXHALO_An1AmpPoolHmB-B_ak1
        47.6%
        36%
        1.0
        HC5FNDRX5_l01_n02_pIXHALO_C24AmpPoolHmB-B_ak1
        46.7%
        35%
        0.9
        HC5FNDRX5_l01_n02_pIXHALO_ColAmpPoolHmB-B_ak1
        47.7%
        36%
        1.3
        HC5FNDRX5_l01_n02_pIXHALO_CviAmpPoolHmB-B_ak1
        47.1%
        36%
        1.0
        HC5FNDRX5_l01_n02_pIXHALO_EriAmpPoolHmB-B_ak1
        46.4%
        35%
        0.9
        HC5FNDRX5_l01_n02_pIXHALO_KyoAmpPoolHmB-B_ak1
        47.1%
        36%
        0.9
        HC5FNDRX5_l01_n02_pIXHALO_LerAmpPoolHmB-B_ak1
        47.1%
        36%
        1.0
        HC5FNDRX5_l01_n02_pIXHALO_ShaAmpPoolHmB-B_ak1
        46.5%
        36%
        0.9
        HC5FNDRX5_l01_n02_undetermined
        69.5%
        42%
        42.7

        Demultiplexing Report


        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool.

        Showing 130/130 rows and 2/2 columns.
        LibraryTotal Read CountPortion (%)
        undetermined_library
        42683608
        11.2
        AtbZIP18_ColAmpPoolHmB-B_ak1
        2375792
        0.6
        AtbZIP18_C24AmpPoolHmB-B_ak1
        1931779
        0.5
        AtbZIP18_EriAmpPoolHmB-B_ak1
        1944682
        0.5
        AtbZIP18_CviAmpPoolHmB-B_ak1
        2078698
        0.5
        AtbZIP18_ShaAmpPoolHmB-B_ak1
        1932358
        0.5
        AtbZIP18_KyoAmpPoolHmB-B_ak1
        1874024
        0.5
        AtbZIP18_LerAmpPoolHmB-B_ak1
        2123348
        0.6
        AtbZIP18_An1AmpPoolHmB-B_ak1
        2487331
        0.7
        AtbZIP18_ColAmpPoolHmB-B_ak2
        2702500
        0.7
        AtbZIP18_C24AmpPoolHmB-B_ak2
        2183834
        0.6
        AtbZIP18_EriAmpPoolHmB-B_ak2
        2213313
        0.6
        AtbZIP18_CviAmpPoolHmB-B_ak2
        2346644
        0.6
        AtbZIP18_ShaAmpPoolHmB-B_ak2
        2204169
        0.6
        AtbZIP18_KyoAmpPoolHmB-B_ak2
        2153695
        0.6
        AtbZIP18_LerAmpPoolHmB-B_ak2
        2409752
        0.6
        AtbZIP18_An1AmpPoolHmB-B_ak2
        2817132
        0.7
        AtbZIP16_ColAmpPoolHmB-B_ak1
        2641994
        0.7
        AtbZIP16_C24AmpPoolHmB-B_ak1
        2078543
        0.5
        AtbZIP16_EriAmpPoolHmB-B_ak1
        2040551
        0.5
        AtbZIP16_CviAmpPoolHmB-B_ak1
        2269803
        0.6
        AtbZIP16_ShaAmpPoolHmB-B_ak1
        2235574
        0.6
        AtbZIP16_KyoAmpPoolHmB-B_ak1
        2034711
        0.5
        AtbZIP16_LerAmpPoolHmB-B_ak1
        2150626
        0.6
        AtbZIP16_An1AmpPoolHmB-B_ak1
        2219940
        0.6
        AtbZIP16_ColAmpPoolHmB-B_ak2
        3230735
        0.8
        AtbZIP16_C24AmpPoolHmB-B_ak2
        2724159
        0.7
        AtbZIP16_EriAmpPoolHmB-B_ak2
        2353255
        0.6
        AtbZIP16_CviAmpPoolHmB-B_ak2
        2695809
        0.7
        AtbZIP16_ShaAmpPoolHmB-B_ak2
        2757759
        0.7
        AtbZIP16_KyoAmpPoolHmB-B_ak2
        2440224
        0.6
        AtbZIP16_LerAmpPoolHmB-B_ak2
        2621629
        0.7
        AtbZIP16_An1AmpPoolHmB-B_ak2
        2614884
        0.7
        AtbZIP2_ColAmpPoolHmB-B_ak1
        2817275
        0.7
        AtbZIP2_C24AmpPoolHmB-B_ak1
        2007786
        0.5
        AtbZIP2_EriAmpPoolHmB-B_ak1
        2016530
        0.5
        AtbZIP2_CviAmpPoolHmB-B_ak1
        2279050
        0.6
        AtbZIP2_ShaAmpPoolHmB-B_ak1
        2149168
        0.6
        AtbZIP2_KyoAmpPoolHmB-B_ak1
        2052713
        0.5
        AtbZIP2_LerAmpPoolHmB-B_ak1
        2210094
        0.6
        AtbZIP2_An1AmpPoolHmB-B_ak1
        2275727
        0.6
        AtbZIP2_ColAmpPoolHmB-B_ak2
        3339702
        0.9
        AtbZIP2_C24AmpPoolHmB-B_ak2
        2445110
        0.6
        AtbZIP2_EriAmpPoolHmB-B_ak2
        2338541
        0.6
        AtbZIP2_CviAmpPoolHmB-B_ak2
        2688221
        0.7
        AtbZIP2_ShaAmpPoolHmB-B_ak2
        2599751
        0.7
        AtbZIP2_KyoAmpPoolHmB-B_ak2
        2423872
        0.6
        AtbZIP2_LerAmpPoolHmB-B_ak2
        2661330
        0.7
        AtbZIP2_An1AmpPoolHmB-B_ak2
        2655287
        0.7
        AtbZIP23_ColAmpPoolHmB-B_ak1
        2945924
        0.8
        AtbZIP23_C24AmpPoolHmB-B_ak1
        1949079
        0.5
        AtbZIP23_EriAmpPoolHmB-B_ak1
        1999734
        0.5
        AtbZIP23_CviAmpPoolHmB-B_ak1
        2359217
        0.6
        AtbZIP23_ShaAmpPoolHmB-B_ak1
        2125976
        0.6
        AtbZIP23_KyoAmpPoolHmB-B_ak1
        2153200
        0.6
        AtbZIP23_LerAmpPoolHmB-B_ak1
        2284294
        0.6
        AtbZIP23_An1AmpPoolHmB-B_ak1
        2421300
        0.6
        AtbZIP23_ColAmpPoolHmB-B_ak2
        2800367
        0.7
        AtbZIP23_C24AmpPoolHmB-B_ak2
        1627063
        0.4
        AtbZIP23_EriAmpPoolHmB-B_ak2
        1731188
        0.5
        AtbZIP23_CviAmpPoolHmB-B_ak2
        2143161
        0.6
        AtbZIP23_ShaAmpPoolHmB-B_ak2
        1859673
        0.5
        AtbZIP23_KyoAmpPoolHmB-B_ak2
        1896984
        0.5
        AtbZIP23_LerAmpPoolHmB-B_ak2
        2130052
        0.6
        AtbZIP23_An1AmpPoolHmB-B_ak2
        2240461
        0.6
        AtbZIP21_ColAmpPoolHmB-B_ak1
        6784929
        1.8
        AtbZIP21_C24AmpPoolHmB-B_ak1
        4867703
        1.3
        AtbZIP21_EriAmpPoolHmB-B_ak1
        4319545
        1.1
        AtbZIP21_CviAmpPoolHmB-B_ak1
        5323548
        1.4
        AtbZIP21_ShaAmpPoolHmB-B_ak1
        4884021
        1.3
        AtbZIP21_KyoAmpPoolHmB-B_ak1
        4673775
        1.2
        AtbZIP21_LerAmpPoolHmB-B_ak1
        5465815
        1.4
        AtbZIP21_An1AmpPoolHmB-B_ak1
        5430208
        1.4
        AtbZIP21_ColAmpPoolHmB-B_ak2
        5374449
        1.4
        AtbZIP21_C24AmpPoolHmB-B_ak2
        3905869
        1.0
        AtbZIP21_EriAmpPoolHmB-B_ak2
        3229050
        0.8
        AtbZIP21_CviAmpPoolHmB-B_ak2
        4025945
        1.1
        AtbZIP21_ShaAmpPoolHmB-B_ak2
        3672842
        1.0
        AtbZIP21_KyoAmpPoolHmB-B_ak2
        3563334
        0.9
        AtbZIP21_LerAmpPoolHmB-B_ak2
        4202847
        1.1
        AtbZIP21_An1AmpPoolHmB-B_ak2
        4096560
        1.1
        AtbZIP22_ColAmpPoolHmB-B_ak1
        3044609
        0.8
        AtbZIP22_C24AmpPoolHmB-B_ak1
        2889265
        0.8
        AtbZIP22_EriAmpPoolHmB-B_ak1
        2319900
        0.6
        AtbZIP22_CviAmpPoolHmB-B_ak1
        2610642
        0.7
        AtbZIP22_ShaAmpPoolHmB-B_ak1
        2762280
        0.7
        AtbZIP22_KyoAmpPoolHmB-B_ak1
        2467573
        0.6
        AtbZIP22_LerAmpPoolHmB-B_ak1
        2628979
        0.7
        AtbZIP22_An1AmpPoolHmB-B_ak1
        2557402
        0.7
        AtbZIP22_ColAmpPoolHmB-B_ak2
        3821105
        1.0
        AtbZIP22_C24AmpPoolHmB-B_ak2
        3682757
        1.0
        AtbZIP22_EriAmpPoolHmB-B_ak2
        2824789
        0.7
        AtbZIP22_CviAmpPoolHmB-B_ak2
        3231990
        0.9
        AtbZIP22_ShaAmpPoolHmB-B_ak2
        3503855
        0.9
        AtbZIP22_KyoAmpPoolHmB-B_ak2
        3021651
        0.8
        AtbZIP22_LerAmpPoolHmB-B_ak2
        3204140
        0.8
        AtbZIP22_An1AmpPoolHmB-B_ak2
        3112908
        0.8
        AtbZIP17_ColAmpPoolHmA-B_ak1
        2500958
        0.7
        AtbZIP17_C24AmpPoolHmA-B_ak1
        2679681
        0.7
        AtbZIP17_EriAmpPoolHmA-B_ak1
        2091220
        0.6
        AtbZIP17_CviAmpPoolHmA-B_ak1
        2604623
        0.7
        AtbZIP17_ShaAmpPoolHmA-B_ak1
        2436492
        0.6
        AtbZIP17_KyoAmpPoolHmA-B_ak1
        2563788
        0.7
        AtbZIP17_LerAmpPoolHmA-B_ak1
        3102045
        0.8
        AtbZIP17_An1AmpPoolHmA-B_ak1
        2330184
        0.6
        AtbZIP17_ColAmpPoolHmA-B_ak2
        2509784
        0.7
        AtbZIP17_C24AmpPoolHmA-B_ak2
        2542170
        0.7
        AtbZIP17_EriAmpPoolHmA-B_ak2
        1861631
        0.5
        AtbZIP17_CviAmpPoolHmA-B_ak2
        2406796
        0.6
        AtbZIP17_ShaAmpPoolHmA-B_ak2
        2266694
        0.6
        AtbZIP17_KyoAmpPoolHmA-B_ak2
        2476417
        0.7
        AtbZIP17_LerAmpPoolHmA-B_ak2
        2838936
        0.7
        AtbZIP17_An1AmpPoolHmA-B_ak2
        2190201
        0.6
        AtbZIP26_ColAmpPoolHmB-B_ak1
        2536767
        0.7
        AtbZIP26_C24AmpPoolHmB-B_ak1
        1698636
        0.4
        AtbZIP26_EriAmpPoolHmB-B_ak1
        1743487
        0.5
        AtbZIP26_CviAmpPoolHmB-B_ak1
        2089531
        0.6
        AtbZIP26_ShaAmpPoolHmB-B_ak1
        1936025
        0.5
        AtbZIP26_KyoAmpPoolHmB-B_ak1
        1895130
        0.5
        AtbZIP26_LerAmpPoolHmB-B_ak1
        2031698
        0.5
        AtbZIP26_An1AmpPoolHmB-B_ak1
        2124373
        0.6
        pIXHALO_ColAmpPoolHmB-B_ak1
        1277425
        0.3
        pIXHALO_C24AmpPoolHmB-B_ak1
        891830
        0.2
        pIXHALO_EriAmpPoolHmB-B_ak1
        858666
        0.2
        pIXHALO_CviAmpPoolHmB-B_ak1
        987540
        0.3
        pIXHALO_ShaAmpPoolHmB-B_ak1
        928196
        0.2
        pIXHALO_KyoAmpPoolHmB-B_ak1
        917055
        0.2
        pIXHALO_LerAmpPoolHmB-B_ak1
        996846
        0.3
        pIXHALO_An1AmpPoolHmB-B_ak1
        1010532
        0.3
        PdaTF1_Pda-B_ak
        3899124
        1.0

        Barcodes of Undetermined Reads


        We have determined the barcodes of your undetermined reads. Here are the top 20 barcodes. The full list is available here. If your libraries are dual indexed, the two indices are concatenated.

        Showing 20/20 rows and 2/2 columns.
        Barcode Sequence(s)CountFrequency (%)
        GGGGGGGGAGATCTCG
        27217977.0
        63.8
        GGGGGGGGTAAGATTA
        403611.0
        0.9
        GGGGGGGGCGTGTGAT
        256713.0
        0.6
        GGGGGGGGTCTTACGG
        254599.0
        0.6
        GGGGGGGGACGTCCTG
        208979.0
        0.5
        GGGGGGGGTGGAAGCA
        187586.0
        0.4
        GGGGGGGGAGTCGAAG
        159685.0
        0.4
        GGGGGGGGGTCAGTAC
        158418.0
        0.4
        GGGGGGGGAAGGCGTA
        156294.0
        0.4
        GGGGGGGGCTCGTTCT
        150605.0
        0.3
        GGGGGGGGGCCTCTAT
        148943.0
        0.3
        GGGGGGGGAGGCTATA
        144663.0
        0.3
        GGGGGGGGAGTTCTCG
        139643.0
        0.3
        GGGGGGGGAGGATAGG
        122649.0
        0.3
        GGGGGGGGCTTCGCCT
        116050.0
        0.3
        GGGGGGGGAACCTCTC
        108231.0
        0.2
        GGGGGGGGAACAGGTG
        98062.0
        0.2
        CGGCTAATGGGGGGGG
        95678.0
        0.2
        GCTATCCTGGGGGGGG
        80724.0
        0.2
        AGAGTAGCGGGGGGGG
        80204.0
        0.2

        Run Statistics

        Showing 1/1 rows and 4/4 columns.
        LaneTotal # of Single-End ReadsTotal # PF Reads% Undetermined% PhiX Aligned
        1.0
        638337024
        379929451
        11.2
        7.2

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Counts

        Sequence counts for each sample. Duplicate read counts are an estimate only.

        This plot show the total number of reads, broken down into unique and duplicate if possible (only more recent versions of FastQC give duplicate info).

        You can read more about duplicate calculation in the FastQC documentation. A small part has been copied here for convenience:

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Sequence Quality Histograms

        The mean quality value across each base position in the read.

        To enable multiple samples to be plotted on the same graph, only the mean quality scores are plotted (unlike the box plots seen in FastQC reports).

        Taken from the FastQC help:

        The y-axis on the graph shows the quality scores. The higher the score, the better the base call. The background of the graph divides the y axis into very good quality calls (green), calls of reasonable quality (orange), and calls of poor quality (red). The quality of calls on most platforms will degrade as the run progresses, so it is common to see base calls falling into the orange area towards the end of a read.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality.

        From the FastQC help:

        The per sequence quality score report allows you to see if a subset of your sequences have universally low quality values. It is often the case that a subset of sequences will have universally poor quality, however these should represent only a small percentage of the total sequences.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called.

        To enable multiple samples to be shown in a single plot, the base composition data is shown as a heatmap. The colours represent the balance between the four bases: an even distribution should give an even muddy brown colour. Hover over the plot to see the percentage of the four bases under the cursor.

        To see the data as a line plot, as in the original FastQC graph, click on a sample track.

        From the FastQC help:

        Per Base Sequence Content plots out the proportion of each base position in a file for which each of the four normal DNA bases has been called.

        In a random library you would expect that there would be little to no difference between the different bases of a sequence run, so the lines in this plot should run parallel with each other. The relative amount of each base should reflect the overall amount of these bases in your genome, but in any case they should not be hugely imbalanced from each other.

        It's worth noting that some types of library will always produce biased sequence composition, normally at the start of the read. Libraries produced by priming using random hexamers (including nearly all RNA-Seq libraries) and those which were fragmented using transposases inherit an intrinsic bias in the positions at which reads start. This bias does not concern an absolute sequence, but instead provides enrichement of a number of different K-mers at the 5' end of the reads. Whilst this is a true technical bias, it isn't something which can be corrected by trimming and in most cases doesn't seem to adversely affect the downstream analysis.

        Click a sample row to see a line plot for that dataset.
        Rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content.

        From the FastQC help:

        This module measures the GC content across the whole length of each sequence in a file and compares it to a modelled normal distribution of GC content.

        In a normal random library you would expect to see a roughly normal distribution of GC content where the central peak corresponds to the overall GC content of the underlying genome. Since we don't know the the GC content of the genome the modal GC content is calculated from the observed data and used to build a reference distribution.

        An unusually shaped distribution could indicate a contaminated library or some other kinds of biased subset. A normal distribution which is shifted indicates some systematic bias which is independent of base position. If there is a systematic bias which creates a shifted normal distribution then this won't be flagged as an error by the module since it doesn't know what your genome's GC content should be.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Base N Content

        The percentage of base calls at each position for which an N was called.

        From the FastQC help:

        If a sequencer is unable to make a base call with sufficient confidence then it will normally substitute an N rather than a conventional base call. This graph shows the percentage of base calls at each position for which an N was called.

        It's not unusual to see a very low proportion of Ns appearing in a sequence, especially nearer the end of a sequence. However, if this proportion rises above a few percent it suggests that the analysis pipeline was unable to interpret the data well enough to make valid base calls.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Sequence Length Distribution

        All samples have sequences of a single length (101bp).

        Sequence Duplication Levels

        The relative level of duplication found for every sequence.

        From the FastQC Help:

        In a diverse library most sequences will occur only once in the final set. A low level of duplication may indicate a very high level of coverage of the target sequence, but a high level of duplication is more likely to indicate some kind of enrichment bias (eg PCR over amplification). This graph shows the degree of duplication for every sequence in a library: the relative number of sequences with different degrees of duplication.

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        In a properly diverse library most sequences should fall into the far left of the plot in both the red and blue lines. A general level of enrichment, indicating broad oversequencing in the library will tend to flatten the lines, lowering the low end and generally raising other categories. More specific enrichments of subsets, or the presence of low complexity contaminants will tend to produce spikes towards the right of the plot.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Overrepresented sequences

        The total amount of overrepresented sequences found in each library.

        FastQC calculates and lists overrepresented sequences in FastQ files. It would not be possible to show this for all samples in a MultiQC report, so instead this plot shows the number of sequences categorized as over represented.

        Sometimes, a single sequence may account for a large number of reads in a dataset. To show this, the bars are split into two: the first shows the overrepresented reads that come from the single most common sequence. The second shows the total count from all remaining overrepresented sequences.

        From the FastQC Help:

        A normal high-throughput library will contain a diverse set of sequences, with no individual sequence making up a tiny fraction of the whole. Finding that a single sequence is very overrepresented in the set either means that it is highly biologically significant, or indicates that the library is contaminated, or not as diverse as you expected.

        FastQC lists all of the sequences which make up more than 0.1% of the total. To conserve memory only sequences which appear in the first 100,000 sequences are tracked to the end of the file. It is therefore possible that a sequence which is overrepresented but doesn't appear at the start of the file for some reason could be missed by this module.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position.

        Note that only samples with ≥ 0.1% adapter contamination are shown.

        There may be several lines per sample, as one is shown for each adapter detected in the file.

        From the FastQC Help:

        The plot shows a cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. Once a sequence has been seen in a read it is counted as being present right through to the end of the read so the percentages you see will only increase as the read length goes on.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Status Checks

        Status for each FastQC section showing whether results seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

        FastQC assigns a status for each section of the report. These give a quick evaluation of whether the results of the analysis seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

        It is important to stress that although the analysis results appear to give a pass/fail result, these evaluations must be taken in the context of what you expect from your library. A 'normal' sample as far as FastQC is concerned is random and diverse. Some experiments may be expected to produce libraries which are biased in particular ways. You should treat the summary evaluations therefore as pointers to where you should concentrate your attention and understand why your library may not look random and diverse.

        Specific guidance on how to interpret the output of each module can be found in the relevant report section, or in the FastQC help.

        In this heatmap, we summarise all of these into a single heatmap for a quick overview. Note that not all FastQC sections have plots in MultiQC reports, but all status checks are shown in this heatmap.

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