FastQCFastQC Report
Sat 24 Aug 2024
HC3CLAFX7_n01_7797-sp-in.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC3CLAFX7_n01_7797-sp-in.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29148466
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGGTGG1882650.64588304578361TruSeq Adapter, Index 7 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGTTGG1441560.49455775820243847TruSeq Adapter, Index 7 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGGTTG833600.28598417494766276TruSeq Adapter, Index 7 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGTTTG707650.24277435388881186TruSeq Adapter, Index 7 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGGGGG440810.15122922763757105TruSeq Adapter, Index 7 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATGGGGGGTGG329540.11305569219320151TruSeq Adapter, Index 7 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGATGG327090.11221516768669748TruSeq Adapter, Index 7 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGTTTGG313190.10744647762938879TruSeq Adapter, Index 7 (97% over 42bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)