FastQCFastQC Report
Sun 10 Apr 2022
HC33GDMXY_n02_PB2_Var.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC33GDMXY_n02_PB2_Var.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58699501
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGAACAATTCGACATTAATTGATGGCCGCCCGAATTCTTTTGGTCG12624312.150667345536719No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA3472420.5915586914444128No Hit
GTCTAAGGATATCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC2582570.4399645577907042No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG2116310.36053287744302975No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT1983040.3378291069288647No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG1961710.33419534520404187No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATT1799200.3065102716971989No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA1789970.3049378562860356No Hit
TCCTTACTCCTCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCT1721160.29321543977009273No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAA1614680.2750755922098895No Hit
GTGTTTATATTGAAGTGTTGCACTTAACCCAAGGAACGTGCTGGGAGCAG1607250.2738098233577829No Hit
GTTTGGAGGTTGCCCGTTAGCACTTCTTCTTCATTCTTGACTGATGATCC1528140.26033270708723744No Hit
TTCCTATTCCACCATGTTACGGCCAGAGGTGATACCATCACTCGGTCTGA1519300.25882673176386967No Hit
TTCCAGAACTGGGGAATTGAATCCATCGACAATGTGATGGGAATGATCGG1405520.2394432620474917No Hit
ATTTGACACTGTCCAAATAATAAAACTTCTCCCCTTTGCTGCTGCTCCAC1343420.22886395576003277No Hit
GTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTAC1330970.2267429837265567No Hit
ATCACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGGCCAGGGTTTGT1319430.22477703856460382No Hit
ACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAATAT1303190.22201040516511375No Hit
CTTCATACCCTTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGTT1262760.2151227827302995No Hit
CTCTTATCCCTCTCAGCGACATCTCCATGCTTGGGGTCATGTCGGGCAGT1258830.2144532710763589No Hit
CCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCAT1258760.21444134593239556No Hit
GGATAAGAGTCAGCAAAATGGGAGTAGATGAATACTCCAGCACGGAGAGA1195420.20365079423758642No Hit
GTCTACGGCTTGTTCCTCAGTTGGATTCTGTCTAAGGATATCCACCATCC1159990.19761496780015217No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA1129810.19247352715996682No Hit
TCTCAGTTCCTTGCGTTTCACTGACTTCTTCGGGAGACAATAGTACGTTC1126800.1919607459695441No Hit
GTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAGGTT1119810.19076993516520693No Hit
ATTATTGCCTCAGCAATTGACTGCTCGTCCCTCCCGCTTACTATCAACTG1116030.1901259773911877No Hit
GGTTGGGAGAAGAGCAACAGCTATTCTCAGAAAGGCAACCAGGAGATTGA1113460.1896881542485344No Hit
GATGTACACTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAAGTT1062080.1809350985794581No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA1060210.180616526876438No Hit
TCTCAGGACAGCAGACTCCACTCCAGATGTGCCTTCATCTGGATCTTCAG1059760.1805398652366738No Hit
ACTCACTATAGAACAATTCGACATTAATTGATGGCCGCCCGAATTCTTTT1047640.17847511173902483No Hit
GTTCCAGCAAATGCGGGATGTGCTTGGGACATTTGACACTGTCCAAATAA1045180.1780560281083139No Hit
TGCTGGAACAGTGTCCTTACAAATCCACTGTACCGGCTTCTGGTTGCCTT1027510.17504578105357319No Hit
GTTCTGGAAAAGCACTTTTGCATCTTTTTGGAAATGCCTCAAGAGTTGGT1015840.1730576891956884No Hit
CTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAAGTTTGATTATC999900.17034216355604112No Hit
ACCCTGATCCTCTCACATTCACAGTCAATGAGGAGAATTGCATCCTACTC992730.1691206880957983No Hit
CTCTTGGAAATGTGCCACAGCACACAGATTGGAGGAGTAAGGATGGTGGA990590.16875611940891966No Hit
GTGTTGACTAGCACTGACTCAGGGCCATTGATCTCCCACATCATTGATGA977060.16645115944000954No Hit
GTCTGATCCAGCATCGTTTGTTTTGCTCCAGAGGGTTTGCCCTTGTTCAT969270.1651240612760916No Hit
TCGGAATACTGCCCGACATGACCCCAAGCATGGAGATGTCGCTGAGAGGG962560.1639809510476077No Hit
GTCCAAATAATAAAACTTCTCCCCTTTGCTGCTGCTCCACCAGAACAGAG955730.16281739771518672No Hit
GACTAGCACTGACTCAGGGCCATTGATCTCCCACATCATTGATGACGAAT954870.16267088880363736No Hit
CTTGATGGTGGCGTACATGCTAGAAAGAGAATTGGTCCGTAAAACAAGGT939650.16007802178761282No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG936080.15946983944548354No Hit
GTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTACGGCCAGAGGTGA934670.1592296329742224No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAATATGGTACCTTCG933660.15905757018275163No Hit
ATTCCAGAGAGGAATGAACAAGGGCAAACCCTCTGGAGCAAAACAAACGA924230.15745108293169305No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT914150.1557338622009751No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC912010.15536929351409648No Hit
GTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGATATGGCCC909580.15495532065936982No Hit
AGTCAACACTTATCAATGGATAATCAGGAACTGGGAAATTGTGAAAATTC909160.15488376979558993No Hit
GTTCTGGTGGAGCAGCAGCAAAGGGGAGAAGTTTTATTATTTGGACAGTG904000.1540047163262938No Hit
GTGCTGGAGTATTCATCTACTCCCATTTTGCTGACTCTTATCCCTCTCAG902490.1537474739350851No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC898310.15303537248127544No Hit
TAATAAAACTTCTCCCCTTTGCTGCTGCTCCACCAGAACAGAGTAGGATG897900.15296552520949028No Hit
ATATTGAAGTGTTGCACTTAACCCAAGGAACGTGCTGGGAGCAGATGTAC888340.15133689126249983No Hit
GTTTCTAGCAGCGATAATCAAACTTTGGTCAACATCATCATTTCTCACTT882970.15042206236131375No Hit
TCTCCCAACCATTGTGAATTCTTCATACCCTTCATGTACTCTTATTTTCA869960.14820568917613117No Hit
ATCAGGAACTGGGAAATTGTGAAAATTCAATGGTCACAAGATCCCACAAT861640.14678830063649093No Hit
GTTAGGGGCGATCTGAACTTTGTCAATAGGGCAAACCAGCGACTGAACCC860790.14664349531693635No Hit
CCTCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCTAATGGGTC860390.14657535163714594No Hit
GACATATGCAAGGCAGCAATAGGGTTGAGGATTAGCTCATCTTTCAGTTT858840.14631129487795816No Hit
ATTCTTGACTGATGATCCGCTTGTCCTTTTGAAAGTGAACCCACCAAAAC857220.14603531297480704No Hit
CCCTTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTT856960.1459910195829433No Hit
GTTTATATTGAAGTGTTGCACTTAACCCAAGGAACGTGCTGGGAGCAGAT856570.14592457949514767No Hit
GTGTCAGCAGACCCATTAGCATCTCTCTTGGAAATGTGCCACAGCACACA855950.14581895679147255No Hit
ATCCTACTCTGTTCTGGTGGAGCAGCAGCAAAGGGGAGAAGTTTTATTAT852260.14519033134540615No Hit
GATCAAGGCAGTTAGGGGCGATCTGAACTTTGTCAATAGGGCAAACCAGC852190.14517840620144284No Hit
GTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAAGG848340.14452252328346027No Hit
GTATTCCGATCATTCCCATCACATTGTCGATGGATTCAATTCCCCAGTTC848090.14447993348359128No Hit
TTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATATCTCTTG844130.14380531105366637No Hit
GTACCGGCTTCTGGTTGCCTTAGGGACAAGAGACTGAAATGGTTCAAATT831300.14161960252438943No Hit
ATTGATGGCCGCCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA823910.14036064804026188No Hit
CTCCAGCACGGAGAGAGTGGTAGTGAGTATTGACCGATTTTTAAGGGTTA812700.13845092141413604No Hit
GGATGTGATTATGGAAGTTGTTTTCCCAAATGAAGTGGGGGCAAGAATAC806500.1373946943773849No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGGCGGCC803840.1369415389067788No Hit
GATTGGAGGAGTAAGGATGGTGGATATCCTTAGACAGAATCCAACTGAGG803260.1368427305710827No Hit
ATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATTGGGCAAGGGGACGTA800280.13633506015664426No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA798880.13609655727737788No Hit
CAATTACAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGATGCAG798100.1359636771017866No Hit
CATCAGGAAGGCAAGAAAAGAACCCCGCACTCAGAATGAAGTGGATGATG797880.13592619807790188No Hit
CAATAGTACGTTCCCTCTTTGATCTCTAACCCTTAAAAATCGGTCAATAC794000.13526520438393505No Hit
GCTAGAAACATAGTAAGAAGAGCAGCAGTGTCAGCAGACCCATTAGCATC784320.13361612733300748No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC783660.13350369026135334No Hit
GGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTATTCTCAGA781670.13316467545439611No Hit
TATTTGGACAGTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACA781320.13310504973457954No Hit
GTTGTAATTGAATACTGGAGAATTGCCTCTTACCAGTATCCTCAACCCTG781160.13307779226266336No Hit
CCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAATATG765610.13042870671081175No Hit
CCCTAAGGCAACCAGAAGCCGGTACAGTGGATTTGTAAGGACACTGTTCC760380.12953772809755232No Hit
GTGTACATCTGCTCCCAGCACGTTCCTTGGGTTAAGTGCAACACTTCAAT756870.1289397673073916No Hit
ATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCACTTCAT745410.12698745088139676No Hit
CTCTTGAGGCATTTCCAAAAAGATGCAAAAGTGCTTTTCCAGAACTGGGG743100.12659392113060722No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA742010.1264082296031784No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA741600.12633838233139325No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC738520.12581367599700718No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAA730640.1244712455051364No Hit
TTTCCAAGAGAGATGCTAATGGGTCTGCTGACACTGCTGCTCTTCTTACT727850.1239959433385984No Hit
GGATGCAGGTGCATTGACTGAAGATCCAGATGAAGGCACATCTGGAGTGG722410.12306918929344901No Hit
CTATCAACTGGATCAATCTCCTGGTTGCCTTTCTGAGAATAGCTGTTGCT720640.12276765351037651No Hit
CTCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT720050.12266714158268568No Hit
ATACAAACCCTGGCCATGCAGATCTCAGTGCCAAGGAGGCACAGGATGTG706490.12035707083779126No Hit
GGTAAGAGGCAATTCTCCAGTATTCAATTACAACAAGGCAACCAAACGAC704540.1200248703988131No Hit
GTAATTGGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTT700780.11938431980878339No Hit
GATCAGGGTTGAGGATACTGGTAAGAGGCAATTCTCCAGTATTCAATTAC700140.11927528992111876No Hit
TTCCAAGAGAGATGCTAATGGGTCTGCTGACACTGCTGCTCTTCTTACTA694230.11826846705221565No Hit
AGTGAGTATTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGGAACGTAC693640.11816795512452481No Hit
CCTCAACCCTGATCCTCTCACATTCACAGTCAATGAGGAGAATTGCATCC690180.1175785122943379No Hit
ATACTCACTACCACTCTCTCCGTGCTGGAGTATTCATCTACTCCCATTTT687740.11716283584761648No Hit
GCAATTGACTGCTCGTCCCTCCCGCTTACTATCAACTGGATCAATCTCCT683460.11643369847385925No Hit
CATCTGGAGTGGAGTCTGCTGTCCTGAGAGGATTTCTCATTTTGGGCAAA681000.11601461484314832No Hit
TCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGA674090.11483743277476924No Hit
CTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGGCGGCCATCAATTA670760.1142701366405142No Hit
GCATATGTCTACGGCTTGTTCCTCAGTTGGATTCTGTCTAAGGATATCCA669280.11401800502528973No Hit
GTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGA668180.11383060990586615No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA666620.11356484955468361No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT664810.11325649940363207No Hit
ACCATCAAGGGAGCAATTTTACAATCCTGGAGCTCTTCTTTCTTCTCTTT660340.1124949937819744No Hit
CTTGTGACCATTGAATTTTCACAATTTCCCAGTTCCTGATTATCCATTGA659980.11243366447016305No Hit
GCCGTAGACATATGCAAGGCAGCAATAGGGTTGAGGATTAGCTCATCTTT658460.11217471848695953No Hit
TTTCAGTCTCTTGTCCCTAAGGCAACCAGAAGCCGGTACAGTGGATTTGT653980.11141150927330712No Hit
CTCCATGCTTGGGGTCATGTCGGGCAGTATTCCGATCATTCCCATCACAT651520.11099242564259618No Hit
ACTCACTACCACTCTCTCCGTGCTGGAGTATTCATCTACTCCCATTTTGC649650.11067385393957609No Hit
GCTGACACTGCTGCTCTTCTTACTATGTTTCTAGCAGCGATAATCAAACT649000.1105631204599167No Hit
GTATTCAATTACAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGA645820.11002137820558305No Hit
GCCTTGATCATGCAATCCTCTTGTGAGAATACCATGGCCACAATTATTGC645620.10998730636568785No Hit
AGCATGGAGATGTCGCTGAGAGGGATAAGAGTCAGCAAAATGGGAGTAGA642800.10950689342316557No Hit
TGTAAGGACACTGTTCCAGCAAATGCGGGATGTGCTTGGGACATTTGACA640990.10919854327211401No Hit
GAACTGAGAGGTTGACAATAACTTATTCGTCATCAATGATGTGGGAGATC639000.10885952846515681No Hit
GTGCTAGTCAACACTTATCAATGGATAATCAGGAACTGGGAAATTGTGAA638660.10880160633733496No Hit
GGATCAGACCGAGTGATGGTATCACCTCTGGCCGTAACATGGTGGAATAG638610.10879308837736115No Hit
TCTCCATGCTTGGGGTCATGTCGGGCAGTATTCCGATCATTCCCATCACA635680.10829393592289652No Hit
GAGTAAGGATGGTGGATATCCTTAGACAGAATCCAACTGAGGAACAAGCC633110.10785611278024324No Hit
GTATTCATCTACTCCCATTTTGCTGACTCTTATCCCTCTCAGCGACATCT633030.10784248404428515No Hit
GATGAATACTCCAGCACGGAGAGAGTGGTAGTGAGTATTGACCGATTTTT632830.10780841220438996No Hit
GGTGATACCATCACTCGGTCTGATCCAGCATCGTTTGTTTTGCTCCAGAG632730.10779137628444235No Hit
CTTATCAATGGATAATCAGGAACTGGGAAATTGTGAAAATTCAATGGTCA631490.10758013087709213No Hit
TCTTACTATGTTTCTAGCAGCGATAATCAAACTTTGGTCAACATCATCAT631380.10756139136514975No Hit
ACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGTTCCTG630270.10737229265373141No Hit
GAGTAGGATGCAATTCTCCTCATTGACTGTGAATGTGAGAGGATCAGGGT629220.10719341549428163No Hit
ACTCTGTTCTGGTGGAGCAGCAGCAAAGGGGAGAAGTTTTATTATTTGGA627990.10698387367892616No Hit
GTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTACAAATCCACT623180.10616444592944667No Hit
GTATCAACTCTCCTCCTTATTTTAACTTGATTTCTGAAGTGGACAGGGCC618670.10539612593980996No Hit
GTTCCTGATTATCCATTGATAAGTGTTGACTAGCACTGACTCAGGGCCAT613880.10458010537431996No Hit
CTCTTATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTTCTTCTTCATTC610870.10406732418389725No Hit
AGTTGATAGTAAGCGGGAGGGACGAGCAGTCAATTGCTGAGGCAATAATT610550.10401280924006492No Hit
ATCCTCAACCCTGATCCTCTCACATTCACAGTCAATGAGGAGAATTGCAT607580.10350684241762123No Hit
GAATAGGAATGGCCCAACAACAAGTACAGTTCATTACCCTAAGGTATATA607330.10346425261775224No Hit
GGTCACAAGATCCCACAATGTTATACAACAAAATGGAATTTGAACCATTT607310.10346084543376272No Hit
TCAATGATGTGGGAGATCAATGGCCCTGAGTCAGTGCTAGTCAACACTTA607180.10343869873783085No Hit
ATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTC601000.10238587888506924No Hit
CTTCAATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTT588860.10031771820343072No Hit
TCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCTAATGGGTCTGCTGAC588610.10027512840356173No Hit
CATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTAT588490.10025468529962461No Hit
TATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACGG588410.10024105656366654No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)