FastQCFastQC Report
Sun 10 Apr 2022
HC33GDMXY_n01_PB2_Var.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC33GDMXY_n01_PB2_Var.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58699501
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGAACAATTCGACATTAATTGATGGCCGCCCGAATTCTTTTGGTCG13498892.2996600942144294No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA3586290.6109574934887436No Hit
GTCTAAGGATATCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC2638650.4495183016973177No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG2156370.3673574669740378No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT2047280.3487729819032022No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG2017970.343779753766561No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA1951400.33243894185744444No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATT1801710.30693787328788363No Hit
TCCTTACTCCTCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCT1704600.29039429142677037No Hit
GTGTTTATATTGAAGTGTTGCACTTAACCCAAGGAACGTGCTGGGAGCAG1703390.29018815679540444No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAA1677990.2858610331287143No Hit
TTCCTATTCCACCATGTTACGGCCAGAGGTGATACCATCACTCGGTCTGA1501390.25577559850125475No Hit
GTTTGGAGGTTGCCCGTTAGCACTTCTTCTTCATTCTTGACTGATGATCC1499140.2553922903024337No Hit
TTCCAGAACTGGGGAATTGAATCCATCGACAATGTGATGGGAATGATCGG1463490.24931898484111475No Hit
GTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTAC1355680.2309525595456084No Hit
ACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAATAT1355000.23083671528996474No Hit
ATTTGACACTGTCCAAATAATAAAACTTCTCCCCTTTGCTGCTGCTCCAC1339790.22824555186593495No Hit
ATCACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGGCCAGGGTTTGT1299400.22136474379909976No Hit
CCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCAT1272900.21685022501298606No Hit
CTTCATACCCTTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGTT1272070.21670882687742096No Hit
CTCTTATCCCTCTCAGCGACATCTCCATGCTTGGGGTCATGTCGGGCAGT1260390.21471903142754145No Hit
GGATAAGAGTCAGCAAAATGGGAGTAGATGAATACTCCAGCACGGAGAGA1248390.2126747210338296No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA1204200.20514654800898566No Hit
GGTTGGGAGAAGAGCAACAGCTATTCTCAGAAAGGCAACCAGGAGATTGA1189830.20269848631251566No Hit
GATGTACACTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAAGTT1185870.20202386388259078No Hit
GTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAGGTT1153410.19649400426760016No Hit
TCTCAGTTCCTTGCGTTTCACTGACTTCTTCGGGAGACAATAGTACGTTC1151650.1961941720765224No Hit
GTCTACGGCTTGTTCCTCAGTTGGATTCTGTCTAAGGATATCCACCATCC1147310.19545481315079663No Hit
ACTCACTATAGAACAATTCGACATTAATTGATGGCCGCCCGAATTCTTTT1134230.1932265148216507No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA1095400.18661146710599805No Hit
TCTCAGGACAGCAGACTCCACTCCAGATGTGCCTTCATCTGGATCTTCAG1074970.1831310286607036No Hit
ATTATTGCCTCAGCAATTGACTGCTCGTCCCTCCCGCTTACTATCAACTG1069130.18213613093576383No Hit
GTTCTGGAAAAGCACTTTTGCATCTTTTTGGAAATGCCTCAAGAGTTGGT1060690.1806982992921865No Hit
CTCTTGGAAATGTGCCACAGCACACAGATTGGAGGAGTAAGGATGGTGGA1043680.17780048930909992No Hit
CTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAAGTTTGATTATC1028570.17522636180501772No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG1013940.172734006716684No Hit
ACCCTGATCCTCTCACATTCACAGTCAATGAGGAGAATTGCATCCTACTC1011010.17223485426221938No Hit
GTTCCAGCAAATGCGGGATGTGCTTGGGACATTTGACACTGTCCAAATAA1006370.1714443875766508No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAATATGGTACCTTCG1003410.17094012434620184No Hit
TGCTGGAACAGTGTCCTTACAAATCCACTGTACCGGCTTCTGGTTGCCTT993860.16931319399120617No Hit
CTTGATGGTGGCGTACATGCTAGAAAGAGAATTGGTCCGTAAAACAAGGT991450.16890262832046904No Hit
GACTAGCACTGACTCAGGGCCATTGATCTCCCACATCATTGATGACGAAT988150.16834044296219827No Hit
TCGGAATACTGCCCGACATGACCCCAAGCATGGAGATGTCGCTGAGAGGG976280.16631827926441828No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT971410.1654886299629702No Hit
GTGTTGACTAGCACTGACTCAGGGCCATTGATCTCCCACATCATTGATGA968240.16494859130063133No Hit
GTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTACGGCCAGAGGTGA967870.1648855583968252No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC967730.16486170810889858No Hit
GTCCAAATAATAAAACTTCTCCCCTTTGCTGCTGCTCCACCAGAACAGAG966720.16468964531742783No Hit
GTGCTGGAGTATTCATCTACTCCCATTTTGCTGACTCTTATCCCTCTCAG963510.1641427922871099No Hit
AGTCAACACTTATCAATGGATAATCAGGAACTGGGAAATTGTGAAAATTC939480.1600490607237019No Hit
GTTTATATTGAAGTGTTGCACTTAACCCAAGGAACGTGCTGGGAGCAGAT928790.15822792088130358No Hit
ATTCCAGAGAGGAATGAACAAGGGCAAACCCTCTGGAGCAAAACAAACGA928010.15809504070571231No Hit
GTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGATATGGCCC920240.15677134972578388No Hit
GTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAAGG914740.15583437412866594No Hit
GTCTGATCCAGCATCGTTTGTTTTGCTCCAGAGGGTTTGCCCTTGTTCAT912380.15543232641790258No Hit
GTTCTGGTGGAGCAGCAGCAAAGGGGAGAAGTTTTATTATTTGGACAGTG911970.15536247914611745No Hit
TAATAAAACTTCTCCCCTTTGCTGCTGCTCCACCAGAACAGAGTAGGATG911010.1551989343146205No Hit
GTGTCAGCAGACCCATTAGCATCTCTCTTGGAAATGTGCCACAGCACACA908290.1547355572920458No Hit
GACATATGCAAGGCAGCAATAGGGTTGAGGATTAGCTCATCTTTCAGTTT903680.1539502013824615No Hit
ATATTGAAGTGTTGCACTTAACCCAAGGAACGTGCTGGGAGCAGATGTAC902570.15376110267104315No Hit
GTTTCTAGCAGCGATAATCAAACTTTGGTCAACATCATCATTTCTCACTT892930.1521188399880946No Hit
GATCAAGGCAGTTAGGGGCGATCTGAACTTTGTCAATAGGGCAAACCAGC877760.14953449093204385No Hit
TCTCCCAACCATTGTGAATTCTTCATACCCTTCATGTACTCTTATTTTCA875870.14921251204503425No Hit
ATCAGGAACTGGGAAATTGTGAAAATTCAATGGTCACAAGATCCCACAAT871150.1484084166235076No Hit
GGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTATTCTCAGA869350.1481017700644508No Hit
CAATTACAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGATGCAG868800.14800807250473902No Hit
GTATTCCGATCATTCCCATCACATTGTCGATGGATTCAATTCCCCAGTTC866660.14764350381786037No Hit
ATCCTACTCTGTTCTGGTGGAGCAGCAGCAAAGGGGAGAAGTTTTATTAT864260.14723464173911802No Hit
TTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATATCTCTTG863090.14703532147573112No Hit
GTTAGGGGCGATCTGAACTTTGTCAATAGGGCAAACCAGCGACTGAACCC858350.1462278188702149No Hit
CATCAGGAAGGCAAGAAAAGAACCCCGCACTCAGAATGAAGTGGATGATG856530.1459177651271686No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC854570.1455838610961957No Hit
CTCCAGCACGGAGAGAGTGGTAGTGAGTATTGACCGATTTTTAAGGGTTA851760.14510515174566815No Hit
ATTCTTGACTGATGATCCGCTTGTCCTTTTGAAAGTGAACCCACCAAAAC849760.14476443334671618No Hit
CCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAATATG846580.14422269109238253No Hit
GATTGGAGGAGTAAGGATGGTGGATATCCTTAGACAGAATCCAACTGAGG835380.14231466805825146No Hit
GGATGTGATTATGGAAGTTGTTTTCCCAAATGAAGTGGGGGCAAGAATAC835030.14225504233843486No Hit
CCCTTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTT834840.14222267409053443No Hit
CCTCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCTAATGGGTC833070.14192113830746195No Hit
CAATAGTACGTTCCCTCTTTGATCTCTAACCCTTAAAAATCGGTCAATAC829020.1412311835495842No Hit
ATTGATGGCCGCCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA824160.14040323784013087No Hit
GTTGTAATTGAATACTGGAGAATTGCCTCTTACCAGTATCCTCAACCCTG821850.14000970808934135No Hit
GTACCGGCTTCTGGTTGCCTTAGGGACAAGAGACTGAAATGGTTCAAATT812100.13834870589445045No Hit
ATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATTGGGCAAGGGGACGTA812040.1383384843424819No Hit
GCTAGAAACATAGTAAGAAGAGCAGCAGTGTCAGCAGACCCATTAGCATC808390.13771667326439452No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA807970.13764512240061463No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC805740.13726522138578318No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC795700.13555481502304423No Hit
GTGTACATCTGCTCCCAGCACGTTCCTTGGGTTAAGTGCAACACTTCAAT793780.13522772536005032No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA793480.13517661760020755No Hit
CCCTAAGGCAACCAGAAGCCGGTACAGTGGATTTGTAAGGACACTGTTCC781760.13318000778234895No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA781000.1330505347907472No Hit
TATTTGGACAGTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACA774460.13193638562617424No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA752740.12823618381355575No Hit
ACCATCAAGGGAGCAATTTTACAATCCTGGAGCTCTTCTTTCTTCTCTTT750200.12780347144688675No Hit
GGTAAGAGGCAATTCTCCAGTATTCAATTACAACAAGGCAACCAAACGAC737450.1256313916535679No Hit
GGATGCAGGTGCATTGACTGAAGATCCAGATGAAGGCACATCTGGAGTGG734490.12512712842311896No Hit
GATCAGGGTTGAGGATACTGGTAAGAGGCAATTCTCCAGTATTCAATTAC734390.12511009250317134No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAA732740.12482899982403597No Hit
CTCTTGAGGCATTTCCAAAAAGATGCAAAAGTGCTTTTCCAGAACTGGGG723710.1232906562527678No Hit
ATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCACTTCAT721900.12298230610171626No Hit
CTCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT712170.1213247110908149No Hit
TTTCCAAGAGAGATGCTAATGGGTCTGCTGACACTGCTGCTCTTCTTACT710220.12099251065183672No Hit
TCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGA709320.12083918737230834No Hit
ATACTCACTACCACTCTCTCCGTGCTGGAGTATTCATCTACTCCCATTTT708000.12061431322900001No Hit
ATACAAACCCTGGCCATGCAGATCTCAGTGCCAAGGAGGCACAGGATGTG707510.12053083722125678No Hit
CCTCAACCCTGATCCTCTCACATTCACAGTCAATGAGGAGAATTGCATCC700550.1193451371929039No Hit
GCCGTAGACATATGCAAGGCAGCAATAGGGTTGAGGATTAGCTCATCTTT696830.11871140097085324No Hit
GAGTAAGGATGGTGGATATCCTTAGACAGAATCCAACTGAGGAACAAGCC694020.1182326916203257No Hit
CTATCAACTGGATCAATCTCCTGGTTGCCTTTCTGAGAATAGCTGTTGCT691690.11783575468554663No Hit
GTAATTGGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTT691360.11777953614971956No Hit
AGTGAGTATTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGGAACGTAC689520.11746607522268376No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGGCGGCC688820.11734682378305054No Hit
GCATATGTCTACGGCTTGTTCCTCAGTTGGATTCTGTCTAAGGATATCCA685490.11677952764879553No Hit
GTATTCAATTACAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGA678480.11558530966046883No Hit
ACTCACTACCACTCTCTCCGTGCTGGAGTATTCATCTACTCCCATTTTGC678410.1155733845165055No Hit
GAACTGAGAGGTTGACAATAACTTATTCGTCATCAATGATGTGGGAGATC674280.11486980102266968No Hit
TTCCAAGAGAGATGCTAATGGGTCTGCTGACACTGCTGCTCTTCTTACTA673520.11474032803106794No Hit
GAGTAGGATGCAATTCTCCTCATTGACTGTGAATGTGAGAGGATCAGGGT667780.11376246622607576No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT666570.11355633159470982No Hit
GTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGA664720.11324116707567924No Hit
GATGAATACTCCAGCACGGAGAGAGTGGTAGTGAGTATTGACCGATTTTT662220.11281526907698926No Hit
AGCATGGAGATGTCGCTGAGAGGGATAAGAGTCAGCAAAATGGGAGTAGA661990.11277608646110977No Hit
GCAATTGACTGCTCGTCCCTCCCGCTTACTATCAACTGGATCAATCTCCT658700.11221560469483378No Hit
CTTGTGACCATTGAATTTTCACAATTTCCCAGTTCCTGATTATCCATTGA658690.112213901102839No Hit
CATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTAT658290.11214575742304862No Hit
TTTCAGTCTCTTGTCCCTAAGGCAACCAGAAGCCGGTACAGTGGATTTGT658190.11212872150310102No Hit
CATCTGGAGTGGAGTCTGCTGTCCTGAGAGGATTTCTCATTTTGGGCAAA658050.11210487121517437No Hit
GTGCTAGTCAACACTTATCAATGGATAATCAGGAACTGGGAAATTGTGAA655790.11171985942435864No Hit
CTCCATGCTTGGGGTCATGTCGGGCAGTATTCCGATCATTCCCATCACAT655600.11168749117645821No Hit
CTTATCAATGGATAATCAGGAACTGGGAAATTGTGAAAATTCAATGGTCA653530.11133484763354291No Hit
TGTAAGGACACTGTTCCAGCAAATGCGGGATGTGCTTGGGACATTTGACA648410.11046260853222586No Hit
GTATTCATCTACTCCCATTTTGCTGACTCTTATCCCTCTCAGCGACATCT643110.10955970477500311No Hit
TCTTACTATGTTTCTAGCAGCGATAATCAAACTTTGGTCAACATCATCAT642850.10951541138313936No Hit
GAATAGGAATGGCCCAACAACAAGTACAGTTCATTACCCTAAGGTATATA632420.1077385649326048No Hit
GTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTACAAATCCACT630670.10744043633352182No Hit
GCCTTGATCATGCAATCCTCTTGTGAGAATACCATGGCCACAATTATTGC629260.10720022986226067No Hit
AGTTGATAGTAAGCGGGAGGGACGAGCAGTCAATTGCTGAGGCAATAATT627610.10691913718312528No Hit
GCTGACACTGCTGCTCTTCTTACTATGTTTCTAGCAGCGATAATCAAACT627460.10689358330320389No Hit
ACTCTGTTCTGGTGGAGCAGCAGCAAAGGGGAGAAGTTTTATTATTTGGA626450.10672152051173314No Hit
GGTGATACCATCACTCGGTCTGATCCAGCATCGTTTGTTTTGCTCCAGAG624810.10644213142459251No Hit
CACTATAGAACAATTCGACATTAATTGATGGCCGCCCGAATTCTTTTGGT620830.10576410181067808No Hit
GGATCAGACCGAGTGATGGTATCACCTCTGGCCGTAACATGGTGGAATAG620060.10563292522708158No Hit
ATCCTCAACCCTGATCCTCTCACATTCACAGTCAATGAGGAGAATTGCAT618740.10540805108377328No Hit
GGTCACAAGATCCCACAATGTTATACAACAAAATGGAATTTGAACCATTT618690.10539953312379947No Hit
TCTCCATGCTTGGGGTCATGTCGGGCAGTATTCCGATCATTCCCATCACA617340.10516954820450689No Hit
GTACATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGC615040.1047777220457121No Hit
CTCTTATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTTCTTCTTCATTC614650.10471128195791647No Hit
GGGTTGAGGATACTGGTAAGAGGCAATTCTCCAGTATTCAATTACAACAA614070.1046124736222204No Hit
GTTCCTGATTATCCATTGATAAGTGTTGACTAGCACTGACTCAGGGCCAT614000.10460054847825709No Hit
ACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGTTCCTG612510.10434671327103787No Hit
GAATGTGAGAGGATCAGGGTTGAGGATACTGGTAAGAGGCAATTCTCCAG610530.1040094020560754No Hit
CTTCAATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTT607220.10344551310580988No Hit
CCTCCAAACACTGAAAATAAGAGTACATGAAGGGTATGAAGAATTCACAA606250.10328026468231816No Hit
ATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTC603420.10279814814780112No Hit
TCAATGATGTGGGAGATCAATGGCCCTGAGTCAGTGCTAGTCAACACTTA598610.10197872039832162No Hit
CCCAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGAGGAATG595160.10139098116012946No Hit
AGCATGTACGCCACCATCAAGGGAGCAATTTTACAATCCTGGAGCTCTTC594000.10119336448873731No Hit
TATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACGG592310.1009054574416229No Hit
GTTGTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCC590360.10057325700264472No Hit
GTATCAACTCTCCTCCTTATTTTAACTTGATTTCTGAAGTGGACAGGGCC590340.10056984981865519No Hit
GTCAAGAATGAAGAAGAAGTGCTAACGGGCAACCTCCAAACACTGAAAAT590230.10055111030671283No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)