FastQCFastQC Report
Fri 13 Dec 2019
HC2GYBCX3_l02_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC2GYBCX3_l02_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7133261
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGNAGAGCGTCGTGTAGGGNAAGANTGTANATCTCNGTGGTCGCCG950151.3319994880321917Illumina Single End PCR Primer 1 (95% over 23bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN353820.49601437547287275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGAGCG276700.0122.545397
GGAAGAG285750.0119.4133455
GAAGAGC287400.0118.393586
CGGAAGA368450.0106.051074
TCGGAAG374400.0104.888863
ATCGGAA372700.0103.896022
GATCGGA376050.0103.7405241
GAGCGTC401650.095.400539
AGAGCGT398500.095.26098
GCGCGTC35900.092.534989
AGCGCGT28950.092.343468
GCTCGGA11800.074.646211
TCGGACG7950.063.628583
GGACGAG7150.062.9743275
CGCGCGT12850.054.487268
CGACTAG3601.5296384E-552.695984130-134
TGCTAGC5406.0199454E-849.18292130-134
CTCGGAC6200.048.4786262
GCTAGCG3508.441884E-443.361267130-134
GATCGTC13800.041.5043229
Produced by FastQC (version 0.11.5)