FastQCFastQC Report
Fri 13 Dec 2019
HC2GYBCX3_l02_n02_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC2GYBCX3_l02_n02_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50093438
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGNAGAGCGTCGTGTAGGGNAAGANTGTANATCTCNGTGGTCGCCG574396311.466497867445232Illumina Single End PCR Primer 1 (95% over 23bp)
AGATCGNAAGAGCGTCGTGTAGGNAAAGNGTGTNGATCTNGGTGGTCGCC1488590.297162674280811Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGTNAGAGCGTCGTGTAGGGNAAGANTGTANATCTCNGTGGTCGCCG821720.1640374533686428Illumina Single End PCR Primer 1 (95% over 21bp)
GATCGGNAGAGCGTCGTGTAGGGNAAGANTGTANATCTCNGTTGTCGCCG555460.11088478295300874Illumina Single End PCR Primer 1 (95% over 23bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGAGC15829650.0161.246666
AAGAGCG15908800.0160.350547
GGAAGAG16031100.0159.40975
GGACGAT147500.0132.082025
GGACGAG208300.0128.876435
ACGAGCG235550.0117.399897
GAGCGTC22342900.0115.937829
AGAGCGT22327300.0115.564688
CGGAAGA23040750.0114.094234
TCGGAAG23253500.0113.163623
GATCGGA23163650.0112.6802141
ATCGGAA23074150.0112.6403962
GACGAGC262850.0111.431376
GCTCGGA446200.0110.50461
TCGGACG318150.0108.567143
CGGACGA331300.0102.625544
ACGATCG200900.0101.614477
GACGATC230050.099.283186
CTCGGAA303450.098.436482
CTCGGAC247050.091.396332
Produced by FastQC (version 0.11.5)