FastQCFastQC Report
Fri 13 Dec 2019
HC2GYBCX3_l02_n02_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC2GYBCX3_l02_n02_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38120459
Sequences flagged as poor quality0
Sequence length151
%GC27

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGNAGAGCGTCGTGTAGGGNAAGANTGTANATCTCNGTGGTCGCCG1576790.41363352943887693Illumina Single End PCR Primer 1 (95% over 23bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGAGCG887100.082.986467
GAAGAGC977700.075.1795656
GAGCGTC1004200.073.3030559
AGAGCGT1024550.071.651918
GGAAGAG1092150.067.702165
TCGGAAG1196250.063.2780083
CGGAAGA1196400.063.2594034
GATCGGA1202650.062.256441
ATCGGAA1250200.059.6229672
GCTCGGA29850.053.035891
TCGGACG23300.043.835643
AGCTCGG18650.037.972065130-134
CTCGGAC16650.036.8060152
GCGTAGT18450.034.3433130-134
AGCGTAG23650.031.520155130-134
AGCGTCC25250.031.23807145
GCGTCGG29400.029.267578145
GATCTCG532650.027.97594830-34
CGGACGA36600.026.859814
AGATCTC576200.026.02008430-34
Produced by FastQC (version 0.11.5)