FastQCFastQC Report
Fri 13 Dec 2019
HC2GYBCX3_l02_n02_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC2GYBCX3_l02_n02_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33651716
Sequences flagged as poor quality0
Sequence length151
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGC44050.018.152895130-134
CGACTAG20709.663225E-615.803064130-134
GCGCTAG44401.8189894E-1214.73529130-134
CGCCTAG47000.014.693488130-134
GCCCTAG44553.092282E-1113.869804130-134
CGGCTAG38601.14214345E-813.182868130-134
TCGGAAG186400.012.0739283
GATCGGA185350.012.0047361
ATCGGAA182900.011.7472442
GCTAGCG46601.2503733E-811.699693130-134
GCTAGCA49803.3323886E-810.947906130-134
AGAGCGT202800.010.7772058
TCGCTAG24350.00910748410.448843130-134
CCTAGCG38552.6096724E-510.371401130-134
AAGAGCG244650.010.3298617
CACCTAG45801.4785473E-610.316848130-134
GTTAGCG48753.2859461E-69.692546130-134
AACTAGT41956.6042674E-59.53081130-134
CCTAGCC61857.7890945E-89.402631130-134
CGAGCTA42758.119185E-59.352457130-134
Produced by FastQC (version 0.11.5)