FastQCFastQC Report
Fri 13 Dec 2019
HC2GYBCX3_l02_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC2GYBCX3_l02_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7133261
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTAATCTCGTATGCC404790.5674683710577813TruSeq Adapter, Index 15 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGTAATCTCGTATGCC353780.4959583001379032TruSeq Adapter, Index 15 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC350800.4917806876826742TruSeq Adapter, Index 12 (100% over 50bp)
TCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA173740.24356321744010206Illumina PCR Primer Index 12 (100% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAATCTCGTATG172370.24164263721739607TruSeq Adapter, Index 8 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTTGTAATCTCGTATG167390.2346612580136911TruSeq Adapter, Index 21 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTTAATCTCGTATGCC155350.21778258218786611TruSeq Adapter, Index 21 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC145590.2041002004553037TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAACCTTGTAATCTCGTATG141800.19878706246694183TruSeq Adapter, Index 16 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCCCTTGTAATCTCGTATGCC125180.17548776078710704TruSeq Adapter, Index 3 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAT31400.0112.622853
AGAGCAC590850.0111.1436548
GATCGTA38350.0110.452831
GATCGGA634650.0107.967811
ATCGGAA643350.0107.886362
TCGGAAG610750.0107.541883
ATCGTAA42050.0106.373842
CGGAAGA625000.0105.358294
CGGAATA33700.0104.9364244
CGTAATA34800.0100.012834
GAGCACA657850.099.5372249
AAGAGCA669800.098.846337
GAAGAGC690200.094.825096
GGAAGAG690600.093.8600545
TCGTAAT38050.089.266243
TATCACA80750.082.206779
CGTAAGA15750.078.097614
TCGTAAG15800.077.850473
GGAATAT47300.067.818015
GATCACA38050.062.2546129
Produced by FastQC (version 0.11.5)