FastQCFastQC Report
Fri 13 Dec 2019
HC2GYBCX3_l02_n01_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC2GYBCX3_l02_n01_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50093438
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC2255527145.02639846759969TruSeq Adapter, Index 12 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG4560550.9104086647037481TruSeq Adapter, Index 12 (100% over 49bp)
GATCGTAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC2974270.5937444341512356TruSeq Adapter, Index 12 (98% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC2671500.5333033839681757TruSeq Adapter, Index 12 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGT2451300.4893455306461497TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCGGTCACCTTGTAATCTCGTATGC2182750.43573571452612214TruSeq Adapter, Index 12 (98% over 50bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1395640.2786073497291202TruSeq Adapter, Index 12 (98% over 50bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1344490.26839643148469866TruSeq Adapter, Index 12 (98% over 50bp)
GATCCGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1304080.2603295066311879TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCCGTCACCTTGTAATCTCGTATGC1289170.25735306887900167TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAACCTCGTATGC1267570.25304112686376207TruSeq Adapter, Index 12 (98% over 50bp)
GATGGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1257300.25099095813707173TruSeq Adapter, Index 12 (98% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1043940.2083985531198717TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTATCTCGTATGCC987630.19715755983847624TruSeq Adapter, Index 12 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAACTCGTATGC974580.194552428204269TruSeq Adapter, Index 12 (98% over 50bp)
GATCAGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC929940.18564108137277383TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTTGTAATCTCGTATGC557680.11132795477124169TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAGCTCGTATGC506480.10110705517956263TruSeq Adapter, Index 12 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGTA754650.0127.7047961
TCGGAAG30851450.0122.210293
ATCGGAA31066450.0121.887112
GATCGGA30689200.0121.840171
CGGAAGA31338350.0120.885564
AGAGCAC32085850.0120.669428
GAAGAGC31859900.0120.531896
GAGCACA32060550.0120.528179
AAGAGCA32316200.0120.091017
GGAAGAG31690900.0119.974125
TCGTAAG481750.0118.541543
ATCGTAA849750.0118.175712
CGTAAGA485050.0117.837764
CGTAATA425750.0103.7581564
GTAAGAG529800.099.87355
TCGTAAT448700.097.847273
TAAGAGC571800.095.817856
TCCGAAG264100.095.071633
GATCCGA235600.094.957731
GATTGGA516950.093.053821
Produced by FastQC (version 0.11.5)