FastQCFastQC Report
Fri 13 Dec 2019
HC2GYBCX3_l02_n01_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC2GYBCX3_l02_n01_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38120459
Sequences flagged as poor quality0
Sequence length151
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC5811271.5244491153687316TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC1973950.057.2668088
GAGCACA1982200.056.934899
GAAGAGC2150250.051.9469036
CGGAAGA2188600.050.1078534
TCGGAAG2197100.049.6249923
AAGAGCA2296850.049.4116747
GATCGGA2255350.047.6280441
GGAAGAG2321400.047.5609175
ATCGGAA2320600.047.254682
GACCGGA22000.028.2176841
GATCGTA131300.023.6944471
TGCCGTC1043300.022.69975545-49
GCCGTCT1020200.022.37468545-49
CTGCTTG1045050.022.35933755-59
ATGCCGT1056550.022.27734845-49
TATGCCG1067000.021.9896445-49
GCTTGAA1064800.021.436855-59
TGCTTGA1103800.021.3476355-59
CCGTCTT1082350.021.21924450-54
CGTATGC1108000.020.6621840-44
Produced by FastQC (version 0.11.5)