FastQCFastQC Report
Fri 13 Dec 2019
HC2GYBCX3_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC2GYBCX3_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10515401
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGNNNNNCNNCG526600.5007892708989414Illumina Single End PCR Primer 1 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG453400.055.160013
CGGAAGA456900.054.8412744
ATCGGAA460650.054.4850232
GATCGGA465550.054.153671
AGAGCGT486750.050.6719258
GAGCGTC484250.050.6303869
AAGAGCG498550.050.081387
GGAAGAG507350.049.4676745
GAAGAGC519400.048.232386
TAGTGTA8458.731149E-1131.335276140-144
CTAGTGT5402.1342348E-631.20339140-144
TACTTAG7151.8409992E-830.299398140-144
TAGGGAG4255.9243036E-428.319044140-144
TACGGTA4457.7514234E-427.04628140-144
TAGTCTG5357.1681454E-526.995726140-144
TACTCGA8257.388371E-826.25948140-144
AGTCTAT8307.833842E-826.101288140-144
TAGTGTC5559.2009934E-526.022905140-144
ACACACG8459.317773E-825.637953140-144
TGACTAG5751.17034106E-425.117762140-144
Produced by FastQC (version 0.11.5)